Surface plasmon resonance immunosensor for label-free detection of BIRC5 biomarker in spontaneously occurring canine mammary tumours

Abstract

We report detection of Baculoviral inhibitor of apoptosis repeat containing-5 (BIRC5) protein biomarker in dog serum by label-free surface plasmon resonance (SPR) immunosensor. Initially, overexpression of BIRC5 in canine mammary tumour (CMT) tissues was confirmed by real-time PCR. Recombinant BIRC5 was produced and protein specific antibodies developed in guinea pig specifically reacted with native protein in immunohistochemistry and immunocytochemistry. SPR immunosensor was developed by fabricating anti-BIRC5 antibodies on gold sensor disc. The equilibrium dissociation constant, (KD = kd/ka) was 12.1 × 10−12 M; which indicates that antibodies are of high affinity with sensitivity in picomolar range. The SPR assay could detect as low as 6.25 pg/ml of BIRC5 protein in a calibration experiment (r2 = 0.9964). On testing real clinical samples, 95% specificity and 73.33% sensitivity were recorded. The average amount of serum BIRC5 in dogs with CMT was 110.02 ± 9.77 pg/ml; whereas, in non-cancerous disease conditions, 44.79 ± 4.28 pg/ml and in healthy dog sera 30.28 ± 2.99 pg/ml protein was detected. The SPR immunosensor for detection of BIRC5 in dog sera is reported for the first time and this may find prognostic and diagnostic applications in management of CMT. In future, ‘on-site’ sensors can be developed using this technique for near-patient testing.

High-Sensitivity Goos-Hänchen Shifts Sensor Based on BlueP-TMDCs-Graphene Heterostructure

“…The GH migration results from the role dispersion of the Fresnel reflection coefficient [2,3]. When the phase of reflection coefficient changes significantly near the critical angle of total reflection, GH effect can be enhanced [4][5][6]. Due to the advantages of GH shift in precision measurement and optical sensing, new attention has been observed [7][8][9].…”

LSPR immuno-sensing based on iso-Y nanopillars for highly sensitive and specific imidacloprid detection

“…For instance, in a recent work 48 where a-lipoic acid activated with EDC-NHS chemistry is taken as an IgG immobilizer, a coverage of 24.4 ng mm À2 is reported, while a gold linked mixture of mercaptoundecanoic acid (MUA) and propionic acid with EDC-NHS activation led to a gold coverage of 1.2 ng mm À2 . 49 A value of 3.4 ng mm À2 , has been found for the IgG mass coverage onto an SPR gold chip functionalized with activated MUA, 50 while a lower coverage of 1.5 ng mm À2 has been found for IgG coated gold nanoparticles via direct immobilization of the antibody. 51 In conclusion, the efficiency of our immobilizing strategy on gold with carbon disulfide is nicely comparable with other ones currently available.…”

Construction of a Prognostic Value Model in Papillary Renal Cell Carcinoma by Immune-Related Genes

“…We then performed Cox and Lasso regression analyses to assess the relationship of these DEIRGs with the prognosis of patients with PRCC, and 4 PDEIRGs of interest (IL13RA2, CCL19, BIRC5, and INHBE) were ultimately determined. All 4 PDEIRGs have been reported to be involved in the immune microenvironment and in ammatory response [29][30][31][32]. IL13RA2 could mediate resistance to sunitinib in certain populations of ccRCC by avoiding sunitinib-induced apoptosis [33].…”