Surface plasmon resonance immunosensor for highly sensitive detection of 2,4,6-trinitrotoluene

Shankaran, Dhesingh Ravi; Gobi, K. Vengatajalabathy; Sakai, Takahisa; Matsumoto, Kiyoshi; Toko, Kiyoshi; Miura, Norio

doi:10.1016/j.bios.2004.06.044

Cited by 105 publications

(55 citation statements)

References 23 publications

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“…However, the detection of small molecule clenbuterol (Mw = 277) by a mass transducer using the direct immunoassay is challenging. In this study, we employ the indirect competitive inhibition immunoassay for detecting the small analyte [6,[33][34][35][36]. This method ensures that the signal response of the mass transducer is large, because the antibody, rather than the antigen, is being measured.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…Therefore, efforts were not focused on sensitization of the SPR immunosensor. However, our group has recently reported ppt level (mass fraction) detection in liquid using a different kind of antibody (10) . Therefore, it is possibile to detect TNT at concentrations lower than ppt level (molar fraction) by utilizing this antibody together with the preconcentrator developed here.…”

Section: Results Of Samplingmentioning

confidence: 99%

Detection of Aromatic Nitro Compounds Using Preconcentrator and SPR Immunosensor

et al. 2006

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“…Different optical [18 -23] or electrochemical [24 -27] methods to detect explosives, such as trinitro toluene (TNT) or trinitro triazine (RDX), were developed in recent years. These included a TNT biosensor based on a displacement assay of a surface-confined anti-TNTantibody by TNT and readout of the detachment of the antibody by surface plasmon resonance (SPR) [28,29], or quartz crystal microbalance (QCM) [29]. Also, a quantum dot-based fluorescent biosensor was developed by the application of antibodyfunctionalized quantum dots as reporter units.…”

Section: Introductionmentioning

confidence: 99%

Detection of Explosives Using Field‐Effect Transistors

2009

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The gate surfaces of ion-sensitive field-effect transistor (ISFET) devices were functionalized with the p-donor units, 6-hydroxydopamine (1) or 4-aminothiophenol (2). Concentration of trinitrotoluene, TNT, on the gate via p-donoracceptor interactions yields charge-transfer complexes that alter the gate potential. This enables the label-free analysis of TNT with a detection limit corresponding to 1 Â 10 À7 M.

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Surface plasmon resonance immunosensor for highly sensitive detection of 2,4,6-trinitrotoluene

Cited by 105 publications

References 23 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Detection of Aromatic Nitro Compounds Using Preconcentrator and SPR Immunosensor

Detection of Explosives Using Field‐Effect Transistors

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