2012
DOI: 10.1016/j.abb.2012.01.006
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Surface plasmon resonance (SPR) studies on the interactions of carotenoids and their binding proteins

Abstract: The xanthophyll carotenoids lutein and zeaxanthin constitute the major carotenoids of the macular pigment in the human retina where they are thought to act in part to prevent light induced oxidative damage associated with age-related macular degeneration (AMD). The highly selective uptake of these pigments is mediated by specific carotenoid-binding proteins (GSTP1 and StARD3) recently identified in our laboratory. Carotenoids are hydrophobic in nature, so we first systematically optimized carotenoid preparatio… Show more

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Cited by 39 publications
(29 citation statements)
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“…A low K D is indicative of stronger interaction between the ligand and the protein. For instance, K D values of StARD3 to lutein and GSTP1 to zeaxanthin were less than 0.6 μM [22,23]. However, binding affinities with carotenoids of a protein such as IRBP, a retinoid and carotenoid transporter that is present in the interphotoreceptor retinal space, was previously determined to be in the 1–2 μM range.…”
Section: Resultsmentioning
confidence: 99%
“…A low K D is indicative of stronger interaction between the ligand and the protein. For instance, K D values of StARD3 to lutein and GSTP1 to zeaxanthin were less than 0.6 μM [22,23]. However, binding affinities with carotenoids of a protein such as IRBP, a retinoid and carotenoid transporter that is present in the interphotoreceptor retinal space, was previously determined to be in the 1–2 μM range.…”
Section: Resultsmentioning
confidence: 99%
“…By contrast, only five of the subjects in the 24 mg group increased by 0.25 OD or more, and there were three non-responders in this group. There are several possible physiological steps, including transport [28], binding [29], and/or perhaps demand for these carotenoids for more immediate uses, such as the reduction of systemic inflammation or oxidation [30], whereby the ultimate function and use of macular carotenoids could be enhanced or compromised. Given all of the health and performance benefits derived from increased systemic and local concentrations of these carotenoids, determining the factors that contribute to absorption, transport, binding, and deposition is perhaps the most urgent scientific question in this area.…”
Section: Discussionmentioning
confidence: 99%
“…SPR analyses were conducted as before (48,49). Briefly, the proteins (human BCO2a, mouse BCO2, and HSA) at 10-50 μg/mL in 10 mM sodium acetate (pH 5.0) were immobilized on the sensor chip surface using standard amine coupling to obtain a density of 10-12 kilo-response units.…”
Section: Methodsmentioning
confidence: 99%