Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology

Abstract

This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties.

Detection of E. coli Bacteria in Milk by an Acoustic Wave Aptasensor with an Anti-Fouling Coating

“…MEG-Cl was synthesized according to previously published methods [28,29]. Anhydrous toluene and pyridine were purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Advances in Electrochemical and Acoustic Aptamer-Based Biosensors and Immunosensors in Diagnostics of Leukemia

“…Sufficiently high density of receptors not only provide higher sensitivity but also minimize undesirable interference of the analyte with the naked biosensor surface [26,27]. At the same time immobilization of antibodies using special linkers that provide antifouling surface properties is among the current trends in biosensor development [16]. The simplest method of Abs immobilization is physical adsorption (Figure 2A).…”

“…In this respect, nucleic acid aptamers are ideal because they allow various chemical modifications that also improve their stability. It is also important to provide antifouling properties of biosensors for their application in complex liquids such as blood or blood plasma [16].…”

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

“…The EMPAS has been also used for the analysis of the interaction of milk and a serum with the surfaces of the crystals covered by antifouling layer. It has been shown that the layer composed of monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl) provides excellent antifouling properties [ 57 ].…”