Surface sperm cell ubiquitination directly impaired blastocyst formation rate after intracytoplasmic sperm injection in pig

Petelák, Aleš; Krylov, Vladimír

doi:10.1016/j.theriogenology.2019.06.014

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…Collectively, the results of the current investigation aimed to devise and optimize intracellular strain-related parameters that turned out to vary depending on different speeds of penetrating the porcine oocytes with the use of robotic (i.e., motorized) micro-manipulators might contribute to improvement of the efficiency of a variety of modern assisted reproductive technologies (ARTs). Taking the above-mentioned findings into consideration, the augmented efficiencies of microsurgical in vitro fertilization by ICSI and cloning by somatic cell nuclear transfer (SCNT) will be of especially great importance not only in pigs but also in other mammalian species [25][26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Oocyte Penetration Speed Optimization Based on Intracellular Strain

et al. 2022

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Oocyte penetration is an essential step for many biological technologies, such as animal cloning, embryo microinjection, and intracytoplasmic sperm injection (ICSI). Although the success rate of robotic cell penetration is very high now, the development potential of oocytes after penetration has not been significantly improved compared with manual operation. In this paper, we optimized the oocyte penetration speed based on the intracellular strain. We firstly analyzed the intracellular strain at different penetration speeds and performed the penetration experiments on porcine oocytes. Secondly, we studied the cell development potential after penetration at different penetration speeds. The statistical results showed that the percentage of large intracellular strain decreased by 80% and the maximum and average intracellular strain decreased by 25–38% at the penetration speed of 50 μm/s compared to at 10 μm/s. Experiment results showed that the cleavage rates of the oocytes after penetration increased from 65.56% to 86.36%, as the penetration speed increased from 10 to 50 μm/s. Finally, we verified the gene expression of oocytes after penetration at different speeds. The experimental results showed that the totipotency and antiapoptotic genes of oocytes were significantly higher after penetration at the speed of 50 μm/s, which verified the effectiveness of the optimization method at the gene level.

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Section: Discussionmentioning

confidence: 99%

Oocyte Penetration Speed Optimization Based on Intracellular Strain

et al. 2022

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“…Ubiquitin acts as a molecular marker and tags proteins for degradation by the proteasome. High sperm surface ubiquitination is coupled with lower developmental competence of pig embryos [ 75 ]. Cryopreservation induced a significant decrease in the percentage of ubiquitinated boar sperm, and the ability of frozen-thawed sperm to capacitate and of the acrosome to react were both influenced by ubiquitination [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide m6A profiling reveals mRNA post-transcriptional modification of boar sperm during cryopreservation

et al. 2021

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Background Cryopreservation induces transcriptomic and epigenetic modifications that strongly impairs sperm quality and function, and thus decrease reproductive performance. N6-methyladenosine (m6A) RNA methylation varies in response to stress and has been implicated in multiple important biological processes, including post-transcriptional fate of mRNA, metabolism, and apoptosis. This study aimed to explore whether cryopreservation induces m6A modification of mRNAs associated with sperm energy metabolism, cryoinjuries, and freezability. Results The mRNA and protein expression of m6A modification enzymes were significantly dysregulated in sperm after cryopreservation. Furthermore, m6A peaks were mainly enriched in coding regions and near stop codons with classical RRACH motifs. The mRNAs containing highly methylated m6A peaks (fts vs. fs) were significantly associated with metabolism and gene expression, while the genes with less methylated m6A peaks were primarily involved in processes regulating RNA metabolism and transcription. Furthermore, the joint analysis of DMMGs and differentially expressed genes indicated that both of these play a vital role in sperm energy metabolism and apoptosis. Conclusions Our study is the first to reveal the dynamic m6A modification of mRNAs in boar sperm during cryopreservation. These epigenetic modifications may affect mRNA expression and are closely related to sperm motility, apoptosis, and metabolism, which will provide novel insights into understanding of the cryoinjuries or freezability of boar sperm during cryopreservation.

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“…In boars, Petelak et al (2019) [ 141 ] found an indirect correlation between the degree of ubiquitinated membrane proteins in the extracellular space of sperm and its capability to induce blastocyst formation in fertilized oocytes, effects that were improved by treatment of sperm with a Ub-blocking antibody [ 141 ]. On the other hand, embryo implantation can be reduced by administrating Ub-neutralizing antibodies in mice [ 142 ].…”

Section: Effects Of Eub In Reproductionmentioning

confidence: 99%

Almost 50 Years of Monomeric Extracellular Ubiquitin (eUb)

Mendoza-Salazar,

Fragozo,

González-Martínez

et al. 2024

Pharmaceuticals

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Monomeric ubiquitin (Ub) is a 76-amino-acid highly conserved protein found in eukaryotes. The biological activity of Ub first described in the 1970s was extracellular, but it quickly gained relevance due to its intracellular role, i.e., post-translational modification of intracellular proteins (ubiquitination) that regulate numerous eukaryotic cellular processes. In the following years, the extracellular role of Ub was relegated to the background, until a correlation between higher survival rate and increased serum Ub concentrations in patients with sepsis and burns was observed. Although the mechanism of action (MoA) of extracellular ubiquitin (eUb) is not yet well understood, further studies have shown that it may ameliorate the inflammatory response in tissue injury and multiple sclerosis diseases. These observations, compounded with the high stability and low immunogenicity of eUb due to its high conservation in eukaryotes, have made this small protein a relevant candidate for biotherapeutic development. Here, we review the in vitro and in vivo effects of eUb on immunologic, cardiovascular, and nervous systems, and discuss the potential MoAs of eUb as an anti-inflammatory, antimicrobial, and cardio- and brain-protective agent.

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Surface sperm cell ubiquitination directly impaired blastocyst formation rate after intracytoplasmic sperm injection in pig

Cited by 10 publications

References 24 publications

Oocyte Penetration Speed Optimization Based on Intracellular Strain

Oocyte Penetration Speed Optimization Based on Intracellular Strain

Transcriptome-wide m6A profiling reveals mRNA post-transcriptional modification of boar sperm during cryopreservation

Almost 50 Years of Monomeric Extracellular Ubiquitin (eUb)

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