Surface structures of gill, scale and erythrocyte of Anabas testudineus exposed to sublethal concentration of cypermethrin

Velmurugan, B.; Selvanayagam, Mariadoss; Ipek, Cengiz Elif; Bilici, Serbest; Satar, Ali

doi:10.1016/j.etap.2014.04.009

Cited by 20 publications

(9 citation statements)

References 17 publications

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“…SEM is an important technique for assessing the effects of environmental stressors http://www.ijt.ir; Vol 10, No 1, Jan-Feb 2016 on gill structures, and has ability to show damage of surface ultrastructure of the branchial epithelium that cannot be revealed by light or transmission electron microscope [24]. In our study, alterations observed on the surface of gills of zebrafish using SEM were in agreement with previous results [25,26]. Several authors have reported histopathological anomalies in the gills of other species exposed to nanoparticles.…”

Section: Discussionsupporting

confidence: 91%

Effects of Short-Term Exposure to Sublethal Concentrations of Silver Nanoparticles on Histopathology and Electron Microscope Ultrastructure of Zebrafish (Danio Rerio) Gills

Mansouri

Ali

2016

IJT

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Background: The increasing use of nanomaterials and nanoproducts has increased the possibility of contamination of the environment, which may have adverse effects on different organisms. The aim of this study was to evaluate the effects of silver nanoparticles on histopathology and gill ultrastructure of zebrafish (Danio rerio) under laboratory conditions. Methods: Zebrafish were exposed to four concentrations of silver nanoparticles (0.0015, 0.00375, 0.0075, and 0.015 mg/l) for a period of 4 days. Gill ultrastructure and histopathological changes were studied using scanning electron microscope and haematoxylin - eosin staining. Results: Exposure to silver nanoparticles significantly (P < 0.001) increased the diameter of gill filaments and secondary lamellae, while silver nanoparticles significantly reduced the length of the secondary gills in zebrafish. Moreover, other changes such as vacuolization, dilated and clubbed tips, hyperplasia, edema, fusion, swelling of mucocytes, hypertrophy, and necrosis were observed. The effects of silver nanoparticles in zebrafish gills were dose dependent. Conclusion: Based on the adverse effects of AgNPs on zebrafish gills, silver nanoparticle solutions can be hazardous pollutants for the environment.

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Section: Discussionsupporting

confidence: 91%

Effects of Short-Term Exposure to Sublethal Concentrations of Silver Nanoparticles on Histopathology and Electron Microscope Ultrastructure of Zebrafish (Danio Rerio) Gills

Mansouri

Ali

2016

IJT

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“…It could be assumed that CPF provided a tactile stimulus due to its abrasive action on the gills, thereby stimulating the mucous-cell population on the lamellae. Release of a profuse amount of mucous has also been reported in other fishes (exposure of Carassius auratus to carbaryl, [25]; Anabas testudineus to cypermethrin, [11]; Anabas testudineus to deltamethrin and permethrin, [26]. Epithelial detachment was followed by hyperplasia characterized by cellular proliferation in the interlamellar region, which has been observed as the most deleterious effect of the toxicant.…”

Section: Discussionmentioning

confidence: 60%

“…Gills serve as sensitive indicators of the toxic effects of pesticide because of their direct contact with ambient conditions, large respiratory surface area, high permeability and characteristic responses of lamellar epithe-lial cells to toxicants. During the present study, it was found that there was loss of uniformity of lamellae, characterized by fusion and swellings [8], which might be due to a loss in structural integrity of the epithelial cells, causing the breakage of primary gill filament from gill arch and pronounced shortening of the secondary lamellae [9][10][11]. The degeneration of epithelial lining resulted in the direct contact of the toxicant with blood vessels, and was responsible for the entry of the toxicant into the blood stream.…”

Section: Discussionmentioning

confidence: 99%

SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos

Kaur

Jindal

2016

ARCH BIOL SCI BELGRA

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We evaluated structural modifications in the branchial architecture of grass carp, Ctenopharyngodon idella, chronically exposed to chlorpyrifos (an organophosphate) using scanning electron microscopy (SEM). Static renewal tests were conducted for 96 h to determine the LC 50 of chlorpyrifos to the fish. Physicochemical analysis of water was done using standard methods. To assess the effect of chronic toxicity, fish were exposed to two sublethal concentrations (1.44 µg/L and 2.41 µg/L) of chlorpyrifos for 15, 30 and 60 days, after which gills were examined by SEM, which revealed changes in gill ultrastructure. Branchial alterations included distorted secondary lamellae in the form of curling and shortening, erosion in a few primary filaments and a wrinkled and denuded epithelial surface. Excessive mucosal openings (mucoid hyperplasia) on the surface were observed in the gills of fish exposed to both concentrations of chlorpyrifos. Alteration in the microridge pattern of pavement cells and cracks on the gill rakers were also observed, and the intensity of the damage was found to be directly related to the toxicant concentration and exposure period. The present study revealed that the assessment of surface morphology can serve as a novel bioindicator of pollution, disease and toxicity.

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“…Scales have been used for classification, identification and growth in studies on different fish. Although all scales come in immediate contact with the environment and the pollutants, they have rarely been used as bioindicators of pollution (Tandon &Johal, 1993 andBabu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Morphological differences of scales and gill rakers used as a taxonomic character in some thick-lip fish species (Family: Labridae), Red Sea, Egypt.

Azab¹,

Khalaf-Allah²

2019

Egypt. J. of Aquatic Biolo. and Fish.

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The present study aimed to study the possibility of using the morphological features of scales and gill rakers as taxonomic characters in some thick-lip fish species, Hemigymnus fasciatus and Hemigymnus melapterus. Fishes were collected from land fish market in Hurghada of Egyptian Red Sea, during the period from April 2016 to May 2017. The scales and the first gill arches of studied species were removed, stained and examined.Scales of H. fasciatus and H. melapterus are mainly of cycloid type, triangular shape and they don't find on operculum (OOP) region. Scale margin is smooth in all regions in H. fasciatus, but it is convex in under pectoral fins in H. melapterus. Scale focus has oval shape in H. fasciatus and elongated oval shape in H. melapterus. Separation line has reversed V shape in all regions in first species and semi-striated shape in the caudal peduncle area in the second one. Granulation area of H. fasciatus has crescent shape but in H. melapterus it has triangle shape.Gill rakers of the anterior row have sharp tips in H. fasciatus and but have forked tips in H. melapterus. Gill rakers of the posterior row have flower like shaped base in H. fasciatus and leaf or cup shaped base in H. melapterus. The anterior row has 22-23 gill rakers in H. fasciatus and it has only 20 gill rakers in H. melapterus. The posterior row has only 16 gill rakers in H. fasciatus and it has 18-20 gill rakers in H. melapterus.In conclusion, the morphology of scales and gill rakers in H. fasciatus and H. melapterus can be used as diagnostic characters (finger print) in their classification.

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Surface structures of gill, scale and erythrocyte of Anabas testudineus exposed to sublethal concentration of cypermethrin

Cited by 20 publications

References 17 publications

Effects of Short-Term Exposure to Sublethal Concentrations of Silver Nanoparticles on Histopathology and Electron Microscope Ultrastructure of Zebrafish (Danio Rerio) Gills

Effects of Short-Term Exposure to Sublethal Concentrations of Silver Nanoparticles on Histopathology and Electron Microscope Ultrastructure of Zebrafish (Danio Rerio) Gills

SEM study of ultrastructural changes in branchial architecture of Ctenopharyngodon idella (Cuvier & Valenciennes) exposed to chlorpyrifos

Morphological differences of scales and gill rakers used as a taxonomic character in some thick-lip fish species (Family: Labridae), Red Sea, Egypt.

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