Surface swarming motility by Pectobacterium atrosepticum is a latent phenotype that requires O antigen and is regulated by quorum sensing

Bowden, Steven D.; Hale, Nicola; Chung, Jade C. S.; Hodgkinson, James T.; Spring, David R.; Welch, Martin

doi:10.1099/mic.0.070748-0

Cited by 23 publications

(22 citation statements)

References 44 publications

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“…Adhesion, biofilm formation and mobility require functional communication systems in several bacterial species (Labbate et al, 2004; Yarwood et al, 2004; Sturme et al, 2005; Rieu et al, 2007; Boles and Horswill, 2008; Fujii et al, 2008; Jayaraman and Wood, 2008; Riedel et al, 2009; Ray and Visick, 2012; Bowden et al, 2013). Public goods are exo-products as for example, virulence factors, surfactants or antibiotics produced and secreted by bacterial populations.…”

Section: Introductionmentioning

confidence: 99%

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

Vivant

Garmyn

Gal

et al. 2014

Front. Cell. Infect. Microbiol.

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In this study, we investigated whether the Agr communication system of the pathogenic bacterium Listeria monocytogenes was involved in adaptation and competitiveness in soil. Alteration of the ability to communicate, either by deletion of the gene coding the response regulator AgrA (response-negative mutant) or the signal pro-peptide AgrD (signal-negative mutant), did not affect population dynamics in soil that had been sterilized but survival was altered in biotic soil suggesting that the Agr system of L. monocytogenes was involved to face the complex soil biotic environment. This was confirmed by a set of co-incubation experiments. The fitness of the response-negative mutant was lower either in the presence or absence of the parental strain but the fitness of the signal-negative mutant depended on the strain with which it was co-incubated. The survival of the signal-negative mutant was higher when co-cultured with the parental strain than when co-cultured with the response-negative mutant. These results showed that the ability to respond to Agr communication provided a benefit to listerial cells to compete. These results might also indicate that in soil, the Agr system controls private goods rather than public goods.

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Section: Introductionmentioning

confidence: 99%

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

Vivant

Garmyn

Gal

et al. 2014

Front. Cell. Infect. Microbiol.

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“…Furthermore, mutation of the rfbP has been demonstrated to alter cell surface properties, resulting in aggregation of bacteria in liquid culture 31 . Aggregation of the rfbP and rfbC mutants was observed after the liquid cultures were incubated on the laboratory bench for a few minutes in 5% or 15% NaCl (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The question of whether a tatB mutation in H. beimenensis affects flagellum synthesis will be an interesting topic for future research. In Pectobacterium atrosepticum and Salmonella enterica , rfbP encodes an undecaprenyl-phosphate galactose phosphotransferase, whereas rfbC encodes a biosynthetic precursor of O-antigen, which is involved in the synthesis of dTDP-L-rhamnose and influences bacterial motility 31,46 . RfbP and RfbC are related to cell envelope biogenesis; mutation of the corresponding genes could cause deficits in cell envelop develop and, consequently, various dysfunctions, resulting in the misregulation of genes and loss of osmoregulation.…”

Section: Discussionmentioning

confidence: 99%

“…RfbP and RfbC are related to cell envelope biogenesis; mutation of the corresponding genes could cause deficits in cell envelop develop and, consequently, various dysfunctions, resulting in the misregulation of genes and loss of osmoregulation. The mutation of rfbP and rfbC in H. beimenensis , S. enterica and P. atrosepticum results in reduced swimming or swarming ability 31,46 .…”

Section: Discussionmentioning

confidence: 99%

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Revealing the Saline Adaptation Strategies of the Halophilic Bacterium Halomonas beimenensis through High-throughput Omics and Transposon Mutagenesis Approaches

Chen

Shyu

et al. 2017

Sci Rep

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Studies on the halotolerance of bacteria are attractive to the fermentation industry. However, a lack of sufficient genomic information has precluded an investigation of the halotolerance of Halomonas beimenensis. Here, we describe the molecular mechanisms of saline adaptation in H. beimenensis based on high-throughput omics and Tn5 transposon mutagenesis. The H. beimenensis genome is 4.05 Mbp and contains 3,807 genes, which were sequenced using short and long reads obtained via deep sequencing. Sixteen Tn5 mutants with a loss of halotolerance were identified. Orthologs of the mutated genes, such as nqrA, trkA, atpC, nadA, and gdhB, have significant biological functions in sodium efflux, potassium uptake, hydrogen ion transport for energy conversion, and compatible solute synthesis, which are known to control halotolerance. Other genes, such as spoT, prkA, mtnN, rsbV, lon, smpB, rfbC, rfbP, tatB, acrR1, and lacA, function in cellular signaling, quorum sensing, transcription/translation, and cell motility also shown critical functions for promoting a halotolerance. In addition, KCl application increased halotolerance and potassium-dependent cell motility in a high-salinity environment. Our results demonstrated that a combination of omics and mutagenesis could be used to facilitate the mechanistic exploitation of saline adaptation in H. beimenensis, which can be applied for biotechnological purposes.

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“…These findings suggest that the absence of O-antigen compromised the competency of proliferation in co-culture in vitro and co-feeding assays in vivo , although the conditions in LB culture and in the gut are quite different. Interestingly, the O-antigen has been associated with the social gliding mobility (cells move as groups) in Myxococcus xanthus [ 38 ] and social swarming mobility in Pectobactrium atrosepticum [ 39 ]. In a community setting, cell-cell interactions may be essential for bacterial growth .…”

Section: Discussionmentioning

confidence: 99%

The waaL gene mutation compromised the inhabitation of Enterobacter sp. Ag1 in the mosquito gut environment

Pei

Jiang

et al. 2015

Parasites Vectors

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BackgroundThe mosquito gut harbors a variety of bacteria that are dynamically associated with mosquitoes in various contexts. However, little is known about bacterial factors that affect bacterial inhabitation in the gut microbial community. Enterobacter sp. Ag1 is a predominant Gram negative bacterium in the mosquito midgut.MethodsIn a mutant library that was generated using transposon Tn5-mediated mutagenesis, a mutant was identified, in which the gene waaL was disrupted by the Tn5 insertion. The waaL encodes O antigen ligase, which is required for the attachment of O antigen to the outer core oligosaccharide of the lipopolysaccharide (LPS).ResultsThe waaL− mutation caused the O antigen repeat missing in the LPS. The normal LPS structure was restored when the mutant was complemented with a plasmid containing waaL gene. The waaL− mutation did not affect bacterial proliferation in LB culture, the mutant cells grew at a rate the same as the wildtype (wt) cells. However, when waaL− strain were co-cultured with the wt strain or complemented strain, the mutant cells proliferated with a slower rate, indicating that the mutants were less competitive than wt cells in a community setting. Similarly, in a co-feeding assay, when fluorescently tagged wt strain and waaL− strain were orally co-introduced into the gut of Anopheles stephensi mosquitoes, the mutant cells were less prevalent in both sugar-fed and blood-fed guts. The data suggest that the mutation compromised the bacterial inhabitation in the gut community. Besides, the mutant was more sensitive to oxidative stress, demonstrated by lower survival rate upon exposure to 20 mM H2O2.ConclusionLack of the O antigen structure in LPS of Enterobacter compromised the effective growth in co-culture and co-feeding assays. In addition, O-antigen was involved in protection against oxidative stress. The findings suggest that intact LPS is crucial for the bacteria to steadily stay in the gut microbial community.

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Surface swarming motility by Pectobacterium atrosepticum is a latent phenotype that requires O antigen and is regulated by quorum sensing

Cited by 23 publications

References 44 publications

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

The Agr communication system provides a benefit to the populations of Listeria monocytogenes in soil

Revealing the Saline Adaptation Strategies of the Halophilic Bacterium Halomonas beimenensis through High-throughput Omics and Transposon Mutagenesis Approaches

The waaL gene mutation compromised the inhabitation of Enterobacter sp. Ag1 in the mosquito gut environment

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