Surface versus bulk activity of lysozyme deposited on hydrogel contact lens materials in vitro

Omali, Negar Babaei; Subbaraman, Lakshman N.; Heynen, Miriam; Ng, Alan; Coles-Brennan, Chantal; Fadli, Zohra; Jones, Lyndon

doi:10.1016/j.clae.2018.03.005

Cited by 13 publications

(13 citation statements)

References 47 publications

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“…The ortho-k lenses used in this study were Brighten 22, and the material generic name was Butyloxyfocon A (Brighten Optix Co., Taipei, Taiwan). The preparation of artificial tear solution with salts and lipids has been published previously [ 24 ], and 2 mg/mL of lysozyme (Sigma-Aldrich, St. Louis, MO, USA) and 0.2 mg/mL of albumin (Sigma-Aldrich) were added into salt-lipid mixed solution. After salts, lipids, and proteins were mixed, 225 or 450 mg of alginate acid (AA, molecular weight is 120,000–190,000 g/mol, Sigma-Aldrich) and lambda-carrageenan (CRG, molecular weight is 560,500–580,500 g/mol, Sigma-Aldrich) were added into 100 mL of solution to make polysaccharide-containing artificial tear solution.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses

Yeh

et al. 2022

Polymers

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The adsorption of tear film compositions such as proteins and lipids on the orthokeratology lenses often lead to infection or corneal damage. In order to investigate whether polysaccharides could prevent tear compositions from being adsorbed on the lens, alginic acid and lambda-carrageenan were added into artificial tear solution. By measuring daily adsorption of cholesterol, lysozyme, and albumin, our results showed that polysaccharides could weakly prevent cholesterol adsorption. In addition, polysaccharides could also reduce albumin deposition over time. Although the effect of polysaccharides on lysozyme adsorption was distinct depending on the concentrations of polysaccharides, the overall results demonstrated that polysaccharides could decrease protein deposition over time. Our results provided an in vitro evidence that polysaccharides may be applied as coating materials on the lens or as the composition of artificial tear solutions or eyedrops, in order to prevent adsorption of tear film compositions that may lead to a reduced incidence of infection or corneal damage for orthokeratology lens wearers.

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Section: Methodsmentioning

confidence: 99%

The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses

Yeh

et al. 2022

Polymers

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“…The contact lens care solution used for cleaning and rinsing was Menicon Care Plus multipurpose solution for all rigid gas permeable lenses (Menicon, Nagoya, Japan). The preparation of artificial tear solution has been published previously [13]. In general, a complex of salt solution was made according to the concentration listed in Table 1 [13].…”

Section: Orthokeratology Lenses Contact Lens Care Solution and Artifi...mentioning

confidence: 99%

“…The preparation of artificial tear solution has been published previously [13]. In general, a complex of salt solution was made according to the concentration listed in Table 1 [13]. The pH value of a complex of salt solution was adjusted to 7.4 and was stored at room temperature for 3 or more days.…”

Section: Orthokeratology Lenses Contact Lens Care Solution and Artifi...mentioning

confidence: 99%

The Effect of Different Cleaning Methods on Protein Deposition and Optical Characteristics of Orthokeratology Lenses

Yeh

Tsao

et al. 2021

Polymers

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Orthokeratology lenses are commonly used for myopia control, especially in children. Tear lipids and proteins are immediately adsorbed when the lens is put on the cornea, and protein deposition may cause discomfort or infection. Therefore, we established an in vitro protein deposition analysis by mimicking the current cleaning methods for orthokeratology lens wearers for both short-term and long-term period. The results showed that the amounts of tear proteins accumulated daily and achieved a balance after 14 days when the lens was rubbed to clean or not. Protein deposition also affected the optical characteristics of the lens regardless of cleaning methods. Our results provided an in vitro analysis for protein deposition on the lens, and they may provide a potential effective method for developing care solutions or methods that can more effectively remove tear components from orthokeratology lenses.

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“…A recent in vitro study has demonstrated that a reduction in metabolic activity and an increase in the release of inflammatory cytokines occurred after human corneal epithelial cells were exposed to denatured lysozyme [61]. Several studies have shown that FDA Group IV materials (including etafilcon A) have the greatest ability to conserve protein configuration and keep denatured lysozyme to low levels [62][63][64][65]. This could partially explain the generally high levels of comfort achieved with etafilcon A lenses [60].…”

Section: Conformational Statementioning

confidence: 99%

Thirty years of ‘quiet eye’ with etafilcon A contact lenses

Efron

Brennan

Chalmers³

et al. 2020

Contact Lens and Anterior Eye

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Surface versus bulk activity of lysozyme deposited on hydrogel contact lens materials in vitro

Cited by 13 publications

References 47 publications

The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses

The Effect of Polysaccharides on Preventing Proteins and Cholesterol from Being Adsorbed on the Surface of Orthokeratology Lenses

The Effect of Different Cleaning Methods on Protein Deposition and Optical Characteristics of Orthokeratology Lenses

Thirty years of ‘quiet eye’ with etafilcon A contact lenses

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