2005
DOI: 10.1203/01.pdr.0000155755.27716.04
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Surfactant-Associated Protein B Kinetics In Vivo in Newborn Infants by Stable Isotopes

Abstract: Surfactant-associated protein B (SP-B) is critical to the biophysical function of pulmonary surfactant. No information is available on SP-B synthesis and kinetics in humans. We administered a 24-h i.v. infusion of 13 C-valine as metabolic precursor of SP-B to six newborn infants (weight 3.5 Ϯ 0.5 kg; age 12 d, range 1-43 d). Three of the study infants also received i.v. 2 H-palmitate to label surfactant disaturated phosphatidylcholine (DSPC). SP-B and DSPC were isolated from tracheal aspirates, and their respe… Show more

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Cited by 20 publications
(38 citation statements)
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“…The transient increase in SP-B content of surfactant after each Infasurf dose (return to baseline by 3 days) is consistent with the reported turnover kinetics observed for endogenous SP-B in stable isotope studies. 37 These observations support the concept that booster surfactant doses should be administered every 2 to 3 days to maintain sufficient SP-B content. This approach increases the alveolar load of surfactant lipid; however, clearance is apparently rapid, as we did not observe increased recovery of surfactant PL in TAF 1 day after a booster dose.…”
Section: Discussionsupporting
confidence: 53%
“…The transient increase in SP-B content of surfactant after each Infasurf dose (return to baseline by 3 days) is consistent with the reported turnover kinetics observed for endogenous SP-B in stable isotope studies. 37 These observations support the concept that booster surfactant doses should be administered every 2 to 3 days to maintain sufficient SP-B content. This approach increases the alveolar load of surfactant lipid; however, clearance is apparently rapid, as we did not observe increased recovery of surfactant PL in TAF 1 day after a booster dose.…”
Section: Discussionsupporting
confidence: 53%
“…Isolation of SP-B from tracheal aspirates SP-B was isolated from tracheal aspirates and hydrolysed as previously described [20]. Individual amino acids were derivatised into their N-acetyl-n-propyl derivatives [21] and the 13 C enrichment of leucine was measured by mass spectrometry (GC-MS; Agilent, Milan, Italy).…”
Section: Analysis Of Tracheal Aspiratesmentioning
confidence: 99%
“…The 2 H enrichment of palmitate-DSPC was analysed by gas chromatography-isotope ratio mass spectrometer (IRMS) and expressed in Dø after correction for isotopic contribution of the derivative group [20]. Urine deuterium enrichment was analysed by a high-temperature conversion elemental analyser coupled with an IRMS (TC-EA-IRMS; Thermo Scientific, Bremen, Germany) [20].…”
Section: Analysis Of Tracheal Aspiratesmentioning
confidence: 99%
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