2021
DOI: 10.1038/s41598-021-82219-y
|View full text |Cite
|
Sign up to set email alerts
|

Surfactant protein A reduces TLR4 and inflammatory cytokine mRNA levels in neonatal mouse ileum

Abstract: Levels of intestinal toll-like receptor 4 (TLR4) impact inflammation in the neonatal gastrointestinal tract. While surfactant protein A (SP-A) is known to regulate TLR4 in the lung, it also reduces intestinal damage, TLR4 and inflammation in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. We hypothesized that SP-A-deficient (SP-A−/−) mice have increased ileal TLR4 and inflammatory cytokine levels compared to wild type mice, impacting intestinal physiology. We found that ileal TLR4 an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
14
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
5
1

Relationship

2
4

Authors

Journals

citations
Cited by 11 publications
(15 citation statements)
references
References 77 publications
(91 reference statements)
1
14
0
Order By: Relevance
“…• These results suggest that SP-A in milk reduces intestinal inflammation either directly or indirectly (by altering immunomodulatory components of milk). (23). We have also shown using a rat model of experimental NEC that human SP-A gavage feeds reduced inflammatory cytokine and TLR4 expression, histological damage, and mortality (27).…”
Section: What Is Newmentioning
confidence: 83%
See 2 more Smart Citations
“…• These results suggest that SP-A in milk reduces intestinal inflammation either directly or indirectly (by altering immunomodulatory components of milk). (23). We have also shown using a rat model of experimental NEC that human SP-A gavage feeds reduced inflammatory cytokine and TLR4 expression, histological damage, and mortality (27).…”
Section: What Is Newmentioning
confidence: 83%
“…cDNA was synthesized from 1 μL mRNA via iScript cDNA Synthesis Kit (#170-8891; Bio-Rad Laboratories, Hercules, CA). RT-PCR analysis was conducted for TLR4, IL-1β, TNF-α, IL-6, and CXCL-1 using primers described previously (23). RT-PCR was performed using LightCycler 96 System (Roche Diagnostics, Indianapolis, IN).…”
Section: Tissue Preparation For Cytokine Analysismentioning
confidence: 99%
See 1 more Smart Citation
“…Assessment of SP-A and cytokine expression. Expression of SP-A was determined via standard reverse transcription PCR analysis, while expression of cytokines IL-1β, IL-6, CXCL1, TNF-α, and IL-10 was determined via real-time quantitative reverse transcription PCR (qRT-PCR) as described previously [17]. Brains were harvested, ash frozen in liquid nitrogen, then transferred to -80°C freezer until ready for RNA isolation.…”
Section: Methodsmentioning
confidence: 99%
“…SP-A and SP-D were found to have pro-in ammatory effects in the colon [15] and vascular system [16]. On the other hand, SP-A and SP-D have been found to attenuate in ammation in the intestine [17][18][19], kidney [20], pancreas [21], fetal tissue [22,23], and vascular system [16]. While the anti-in ammatory effects of SP-A in the CNS have not been directly investigated, several previous studies have explored SP-A expression in the context of multiple CNS disease processes [16, [24][25][26].…”
Section: Introductionmentioning
confidence: 99%