Surgical sponge associated with platelets rich plasma in skin mesh grafts and layer in rabbits (Oryctolagus cuniculus)

Abstract

The aim of this study was to evaluate of the efficacy of PRP employment associated with surgical sponges to improve the integration of the graft in the recipient bed. It was held at the Veterinary Hospital UNESP, Campus of Jaboticabal - SP, a study of 64 rabbits, divided into eight groups with eight animals. The groups were divided in control with saline solution 0,9%, control with PRP both without the sponge, surgical sponge with PRP, surgical sponge without PRP, and were used mesh and layer grafts in the respective groups. The data were submitted to statistical analysis (paired t-test, Kruskal-Wallis test, with subsequent use of the multiple comparison tests of Dunn, analysis of variance (F) test, Tukey test, P< 0.05). Edema and exudate with 3 and 3 and 7 days (P= 0,03 e P= 0,0049); coloring on the 14th day (P= 0,0001); cosmetic appearance on the 7th and 14th day (P= 0,0026 and P= 0,0001); mononuclear cells (P= 0,01) and polymorphonuclear (P= 0,01); fibroblast proliferation (P= 0,01); collagenous (P= 0,05); hemorrhage (P-007); necrosis and re-epithelialization (P= 0,2928 and P= 0,1). We concluded that the use of Platelet Rich Plasma Gel on skin grafts associated with a sponge as a compressive dressing promote the skin graft survival without a previous granulation tissue.

Comparison between continuous and pulsed low-intensity laser on the healing of skin grafts applied to recently created wounds in rabbits (Oryctolagus cuniculus)

“…Although, visibly distinct, the macroscopic variables corresponding to the clinical evolution of the skin grafts were not quantified or statistically analyzed in this study. The subjectivity and high degree of observational interference in this type of analysis make microscopic evaluation more relevant, as demonstrated in other studies (STANLEY et al, 2013;PAZZINI et al, 2016;REIS FILHO et al, 2017). However, we believe it is relevant to report these observations here, because they are parameters found by lab assistants while changing the dressings of the skin grafts.…”

Skin graft associated with platelet-rich plasma in correcting extensive injuries resulting from the resection of skin cancer chemically induced in rats

“…Thus, although no significant difference existed, the results of the current study were consistent with those of Pazzini et al . 30 , who used PRP gel on cutaneous grafts associating surgical sponges as a compressive dressing and concluded that it favored healing and integration of the graft into the recipient bed without the presence of granulation tissue.…”

“…A cutaneous graft was subsequently obtained in the dorsal region of the thorax. A cutaneous incision measuring 1 cm 2 was formed, and measured 6 cm in the caudal direction to the spinal process of the first cervical vertebra. The depth limits were determined based on the anatomical references described in the literature 13 .…”

“…The growth of reconstructive surgery in veterinary medicine has occurred because of the increased survival of patients and owners seek the treatment and diagnosis of their animals’ diseases. It is important to emphazise that reconstructive surgery aims to repair defects that have a great loss of continuity of the integument and compromise healing, and to provide the patient with better aesthetic and functional results 1 , 2 .…”