Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study

Abstract

Background and objectivesThis study aimed to establish a mathematical survival model database containing cell-specific coefficients from human digestive system cells exposed to electroporation pulses (EPs).Materials and methodsA total of 20 types of human digestive system cell lines were selected to investigate the effect of EPs on cell viability. Cell viability was measured after exposure to various pulse settings, and a cell survival model was established using the Peleg–Fermi model. Next, the cell-specific coefficients of each cell line were determined.ResultsCell viability tended to decrease when exposed to stronger electric field strength (EFS), longer pulse duration, and more pulse number, but the decreasing tendency varied among different cell lines. When exposed to a lower EFS (<1,000 V/cm), only a slight decrease in cell viability occurred. All cell lines showed a similar tendency: the extent of electrical injury (EI) increased with the increase in pulse number and duration. However, there existed differences in heat sensitivity among organs.ConclusionsThis database can be used for the application of electroporation-based treatment (EBT) in the digestive system to predict cell survival and tissue injury distribution during the treatment.

From barriers to novel strategies: smarter CAR T therapy hits hard to tumors

“…These limitations have been and can be tackled in many ways, one being by incorporating conventional anti-cancer drugs (83). The findings suggest approaching certain targeted tumors by combining specific mathematical and statistical computer models that have shown to improve the desired result and efficacy of transfecting CAR T through electroporation (84,85). Perfusing the targeted organ or tissue or the surrounding vessels could also enhance the outcome.…”