Survival of E. coli and Enterococcus spp. derived from pig slurry in soils of different texture

Cools, Danielle; Merckx, Roel; Vlassak, K.; Verhaegen, Jan

doi:10.1016/s0929-1393(00)00133-5

Cited by 151 publications

(108 citation statements)

References 22 publications

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“…Additionally, ermB was only detected in one interband sample in the first year of the crop rotation, while Garder et al (2014) detected ermB and ermF at quantifiable levels in every interband sample in the two previous years (fall 2010-spring 2012). The drought conditions in the summer of 2012 in Northeast Iowa may have caused additional stress to bacteria hosting the resistance genes and therefore hastened the return of overall concentrations to those found in no-manure control plot soils (Cools et al, 2001;Byappanahalli et al, 2012). The reduction in ermB and ermF genes in soil over winter are similar to those reported previously (Garder et al, 2014;Fahrenfeld et al, 2014).…”

Section: Discussionsupporting

confidence: 76%

Fate and transport of tylosin-resistant bacteria and macrolide resistance genes in artificially drained agricultural fields receiving swine manure

Luby

Moorman

Soupir

2016

Science of The Total Environment

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Application of manure from swine treated with antibiotics introduces antibiotics and antibiotic resistance genes to soil with the potential for further movement in drainage water, which may contribute to the increase in antibiotic resistance in non-agricultural settings. We compared losses of antibiotic-resistant Enterococcus and macrolide-resistance (erm and msrA) genes in water draining from plots with or without swine manure application under chisel plow and no till conditions. Concentrations of ermB,ermC and ermF were all > 10 9 copies g − 1 in manure from tylosin-treated swine, and application of this manure resulted in short-term increases in the abundance of these genes in soil. Abundances of ermB, ermC and ermF in manured soil returned to levels identified in non-manured control plots by the spring following manure application. Tillage practices yielded no significant differences (p > 0.10) in enterococci or erm gene concentrations in drainage water and were therefore combined for further analysis. While enterococci and tylosin-resistant enterococci concentrations in drainage water showed no effects of manure application, ermB and ermF concentrations in drainage water from manured plots were significantly higher (p < 0.01) than concentrations coming from non-manured plots. ErmB and ermF were detected in 78% and 44%, respectively, of water samples draining from plots receiving manure. Although ermC had the highest concentrations of the three genes in drainage water, there was no effect of manure application on ermC abundance. MsrA was not detected in manure, soil or water. This study is the first to report significant increases in abundance of resistance genes in waters draining from agricultural land due to manure application. H I G H L I G H T S• Higher levels of ermB and ermF in drainage are attributable to manure.• Fall manure application resulted in transient increases in soil enterococci.• Manure application did not affect enterococci levels in drainage water. Application of manure from swine treated with antibiotics introduces antibiotics and antibiotic resistance genes to soil with the potential for further movement in drainage water, which may contribute to the increase in antibiotic resistance in non-agricultural settings. We compared losses of antibiotic-resistant Enterococcus and macrolide-resistance (erm and msrA) genes in water draining from plots with or without swine manure application under chisel plow and no till conditions. Concentrations of ermB, ermC and ermF were all N10 9 copies g −1 in manure from tylosin-treated swine, and application of this manure resulted in short-term increases in the abundance of these genes in soil. Abundances of ermB, ermC and ermF in manured soil returned to levels identified in non-manured control plots by the spring following manure application. Tillage practices yielded no significant differences (p N 0.10) in enterococci or erm gene concentrations in drainage water and were therefore combined for further analysis. While enterococci and tylosin...

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Section: Discussionsupporting

confidence: 76%

Fate and transport of tylosin-resistant bacteria and macrolide resistance genes in artificially drained agricultural fields receiving swine manure

Luby

Moorman

Soupir

2016

Science of The Total Environment

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“…Survival of Enterococcus spp. was longer than that of E. coli at 5 1C, while the opposite was true at 15 1C and 25 1C (Cools et al, 2001). However, these authors also reported that while Enterococcus spp.…”

Section: Persistence Of Pathogens In the Environmentmentioning

confidence: 83%

“…Soil parameters that modify survival of non-indigenous bacteria and viruses include soil pH, soil water content, organic matter content, soil texture, temperature, availability of nutrients, adsorption properties of the soil (Gerba et al, 1975;MacLean, 1983;Cools et al, 2001). Bacteria can survive longer in cold soils and fine textured soils (Fenlon et al, 2000).…”

Section: Persistence Of Pathogens In the Environmentmentioning

confidence: 99%

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Development of a risk-based index for source water protection planning, which supports the reduction of pathogens from agricultural activity entering water resources

Goss

Richards

2008

Journal of Environmental Management

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Source water protection planning (SWPP) is an approach to prevent contamination of ground and surface water in watersheds where these resources may be abstracted for drinking or used for recreation. For SWPP the hazards within a watershed that could contribute to water contamination are identified together with the pathways that link them to the water resource. In rural areas, farms are significant potential sources of pathogens. A risk-based index can be used to support the assessment of the potential for contamination following guidelines on safety and operational efficacy of processes and practices developed as beneficial approaches to agricultural land management. Evaluation of the health risk for a target population requires knowledge of the strength of the hazard with respect to the pathogen load (mass Â concentration). Manure handling and on-site wastewater treatment systems form the most important hazards, and both can comprise confined and unconfined source elements. There is also a need to understand the modification of pathogen numbers (attenuation) together with characteristics of the established pathways (surface or subsurface), which allow the movement of the contaminant species from a source to a receptor (water source). Many practices for manure management have not been fully evaluated for their impact on pathogen survival and transport in the environment. A key component is the identification of potential pathways of contaminant transport. This requires the development of a suitable digital elevation model of the watershed for surface movement and information on local groundwater aquifer systems for subsurface flows. Both require detailed soils and geological information. The pathways to surface and groundwater resources can then be identified. Details of land management, farm management practices (including animal and manure management) and agronomic practices have to be obtained, possibly from questionnaires completed by each producer within the watershed. To confirm that potential pathways are active requires some microbial source tracking. One possibility is to identify the molecular types of Escherichia coli present in each hazard on a farm. An essential part of any such index is the identification of mitigation strategies and practices that can reduce the magnitude of the hazard or block open pathways. r

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“…However, these relationships may not be representative of environments dominated by other manure sources, soil types, or land-uses. Our study was conducted on loamy soils, which have been shown to favor ENT survival, while sandy soils favor EC survival (Cools et al, 2001). In an urban watershed primarily influenced by stormwater, Krometis et al (2010) found Spearman's coefficients of 0.51 for incidence of EC and Salmonella and 0.48 for ENT and Salmonella (p < 0.0001).…”

Section: Correlation Analysesmentioning

confidence: 87%

Effects of tillage and poultry manure application rates on Salmonella and fecal indicator bacteria concentrations in tiles draining Des Moines Lobe soils

Hruby

Soupir

Shelley

et al. 2016

Journal of Environmental Management

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Application of poultry manure (PM) to cropland as fertilizer is a common practice in artificially drained regions of the Upper Midwest United States. Tile-waters have the potential to contribute pathogenic bacteria to downstream waters. This 3-year study (2010)(2011)(2012) was designed to evaluate the impacts of manure management and tillage practices on bacteria losses to drainage tiles under a wide range of field conditions. PM was applied annually in spring, prior to planting corn, at application rates ranging from 5 to 40 kg/ha to achieve target rates of 112 and 224 kg/ha nitrogen (PM1 and PM2). Control plots received no manure (PM0). Each treatment was replicated on three chisel-plowed (CP) plots and one no-till (NT) plot. Tilewater grab samples were collected weekly when tiles were flowing beginning 30 days before manure application to 100 days post application, and additional grab samples were obtained to target the full spectrum of flow conditions. Manure and tile-water samples were analyzed for the pathogen,Salmonella spp. (SALM), and fecal indicator bacteria (FIB), Escherichia coli (EC), and enterococci (ENT). All three bacterial genera were detected more frequently, and at significantly higher concentrations, in tile-waters draining NT plots compared to CP plots. Transport of bacteria to NT tiles was most likely facilitated by macropores, which were significantly more numerous above tiles in NT plots in 2012 as determined by smoke-testing. While postmanure samples contained higher concentrations of bacteria than pre-manure samples, significant differences were not seen between low (PM1) and high (PM2) rates of PM application. The highest concentrations were observed under the NT PM2 plot in 2010 (6.6 × 10 3 cfu/100 mL EC, 6.6 × 10 5 cfu/100 mL ENT, and 2.8 × 10 3 cfu/100 mL SALM). Individual and 30-day geometric mean ENT concentrations correlated more strongly to SALM than EC; however, SALM were present in samples with little or no FIB. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. b s t r a c tApplication of poultry manure (PM) to cropland as fertilizer is a common practice in artificially drained regions of the Upper Midwest United States. Tile-waters have the potential to contribute pathogenic bacteria to downstream waters. This 3-year study (2010e2012) was designed to evaluate the impacts of manure management and tillage practices on bacteria losses to drainage tiles under a wide range of field conditions. PM was applied annually in spring, prior to planting corn, at application rates ranging from 5 to 40 kg/ha to achieve target rates of 112 and 224 kg/ha nitrogen (PM1 and PM2). Control plots received no manure (PM0). Each treatment was replicated on three chisel-plowed (CP) plots and one no-till (NT) plot. Tile-water grab samples were collected weekly when tiles were flowing beginning 30 days before manure application to 100 days post application, and addition...

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Survival of E. coli and Enterococcus spp. derived from pig slurry in soils of different texture

Cited by 151 publications

References 22 publications

Fate and transport of tylosin-resistant bacteria and macrolide resistance genes in artificially drained agricultural fields receiving swine manure

Fate and transport of tylosin-resistant bacteria and macrolide resistance genes in artificially drained agricultural fields receiving swine manure

Development of a risk-based index for source water protection planning, which supports the reduction of pathogens from agricultural activity entering water resources

Effects of tillage and poultry manure application rates on Salmonella and fecal indicator bacteria concentrations in tiles draining Des Moines Lobe soils

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