Recently, the emergence of the COVID-19 has caused a high acceleration towards the use of mobile learning applications in learning and education. Investigation of the adoption of mobile learning still needs more research. Therefore, this study seeks to understand the influencing factors of mobile learning adoption in higher education by employing the Information System Success Model (ISS). The proposed model is evaluated through an SEM approach. Subsequently, the findings show that the proposed research model of this study could explain 63.9% of the variance in the actual use of mobile learning systems, which offers important insight for understanding the impact of educational, environmental, and quality factors on mobile learning system actual use. The findings also indicate that institutional policy, change management, and top management support have positive effects on the actual use of mobile learning systems, mediated by quality factors. Furthermore, the results indicate that factors of functionality, design quality, and usability have positive effects on the actual use of mobile learning systems, mediated by student satisfaction. The findings of this study provide practical suggestions, for designers, developers, and decision makers in universities, on how to enhance the use of mobile learning applications and thus derive greater benefits from mobile learning systems.