Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids

Abstract

We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.

Appearance, molecule identification, and phylogenetic analysis of the three Leonurus species used for the treatment of gynecological diseases

“…2.9 DNA extraction, identi cation, and sequence cloning validation of the selected two hypervariable regions Total genomic DNAs of L. japonicus and L. cardiaca were extracted using the plant genomic DNA kit (Tiangen Biotech, Beijing, China), while that of L. sibiricus was extracted by the CTAB method (Nishii et al, 2023). The DNA purity and ampli cation products were detected by 1.0% agarose gel electrophoresis stained with ethidium bromide alongside a 100 bp ladder (New England Biolabs, Ipswitch, MA, USA) using the DNA marker as the reference (Takara) (Asad et al, 2023). The DNA concentration was determined by using a Nanodrop spectrophotometer 2000 (Thermo, Waltham, Massachusetts, USA) (Butler et al, 2022).…”