SV-40 transformation: effect on GM2 ganglioside in cultured cell lines

Hoffman, Linda M.; Brooks, Steven E.; Stein, Maxine R.; Schneck, Larry

doi:10.1016/0005-2760(91)90060-u

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…The enhanced synthesis of GM2, GalNAc-GM1b and GalNAcGD1a was largely dependent on gene-expression of GalNAc-transferase, but the relative amounts of them differed among cell lines, probably due to the differences in their baseline metabolic pathways. One can suggest that enhanced synthesis of gangliosides with GM2-determinant in cervical carcinoma-derived cell lines is triggered by infection with papilloma virus, analogously to in the case of fibroblasts and neural cells after infection with SV-40 virus [20]. Papilloma viruses 16 and 18 were detected in SKG-IIIb and Boku cells, and Hela and HCSC-1 cells, respectively, but not in HKUSP, OMC-4 and HCA-1 cells.…”

Section: Discussionmentioning

confidence: 91%

“…In particular, GalNAc-GD1a with GM2-determinant, which is a minor constituent in the human brain and is an antigen of autoimmune peripheral neuropathies [18,19], was detected in relatively high amounts in three out of seven cell lines. Since elevated amounts of GM2 have been previously reported as an event after SV-40 virus-dependent transformation of human fibroblasts and neural cells, the expression of unique gangliosides with GM2 determinant in cervical carcinoma-derived cell lines is probably a papilloma virus-related phenomenon, which is not observed in endometrial and ovarian carcinomas-derived cell lines [20].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

et al. 2016

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Monoclonal antibody YHD-06 generated by immunization with GM2 reacted with gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, among which GalNAc-GD1a was characterized as an antigen of autoimmune peripheral neuropathies including Guillain-Barré syndrome. When glycolipids were examined by TLC-immunostaining with YHD-06 in seven human cervical carcinoma-derived cell lines, GM2 was found in all cell lines, amounting to 15.5 % to 57.5 % of total gangliosides. Whereas GalNAc-GD1a was present in three cell lines, amounting to 5.4-17.5 % of total gangliosides, and GalNAc-GM1b in four cell lines in amounts of less than 2 %. The elevated amounts of gangliosides with GM2 determinant were closely correlated with the relative intensities of gene expression of GalNAc transferase, this being characteristic of cervical carcinoma-derived cells. However, in tissues from patients with several histological types of cervical carcinomas, GM3 was ubiquitously expressed in amounts of more than 66 % of total gangliosides, GM2 was expressed in only five of 15 tissues, and both GalNAc-GM1b and GalNAc-GD1a were not even detected in trace amounts. Since GM1 was detected in all tissues in amounts of less than 0.06 μg/mg dried tissue, all cervical carcinoma tissues were revealed to exhibit GM2 synthesis, indicating that enhanced synthesis of gangliosides with GM2 determinant is a characteristic of cultivated cells in vitro. Similarly, although I(3)SO3-GalCer was not present in the squamous cell carcinoma (SCC) tissues, SCC-derived cells selectively expressed II(3)SO3-LacCer. Since enhanced synthesis of GM2 has been reported in SV-40 virus-transfected fibroblasts, papilloma virus might be involved in the expression of GM2 in cervical carcinoma-derived cells.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

et al. 2016

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“…28 Fetal brain cells infected with SV40 virus contained elevated levels of GM2. 29 It is possible that infection of human fibroblasts with CMV leads to upregulation of GM2 in the cell membrane, which makes GM2 immunogenic. Another possibility is that CMV infection of a cell leads to the exposure of cryptic antigens.…”

Section: Figure 2 Specific Inhibition Of Anti-gm2 Reactivity By Cytomentioning

confidence: 99%

Cross-reactive antibodies against GM2 and CMV-infected fibroblasts in Guillain–Barré syndrome

Ang¹,

Jacobs²,

Brandenburg³

et al. 2000

Neurology

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“…The activities of papilloma viruses (PV)-16 and -18, which are responsible for carcinogenesis of cervical cells, were also reported to affect the lipid compositions including glycerophospholipids and glycosphingolipids on lipidomic analysis, the difference between PV-16 and PV-18 being favorable and poor prognoses, respectively [ 5 ]. Since enhanced synthesis of GM2 has been reported in fibroblast and neural cells after infection with SV-40 virus [ 6 ], the ubiquitous high expression of gangliosides with GM2-determinant in cervical carcinoma-derived cells was thought to be due to infection with PV [ 3 ]. Since there is a report that PV in cervical carcinoma patients disappeared in their cancerous tissues and sera, analyses of PV in transplanted tissues and human tissues is now in progress in our laboratory.…”

Section: Discussionmentioning

confidence: 99%

Reduced expression of gangliosides with GM2-determinant in cervical carcinoma-derived cells after subcutaneous transplantation into nude mice

et al. 2023

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Human cervical carcinoma-derived cell lines have been frequently found to contain gangliosides with GM2-determinant, i.e., GM2, GalNAc-GM1b and GalNAc-GD1a, but GM2 was only detected in 5 of 15 tissues, and GalNAc-GM1b and GalNAc-GD1a were not found in any tissues from patients with several histological types of cervical carcinomas. To further characterize the ganglioside expression in cervical carcinomas, cells were grown by subcutaneous transplantation into nude mice, and gangliosides were quantitated by TLC-immunostaining with the anti-GM2 (YHD-06) antibody and a newly developed anti-GM3 (5H6) antibody, which reacts with GM3 and GM1b, but not with GD1a. Gangliosides with GM2-determinant in cells disappeared in transplanted cells, and the amount of GM3, a precursor for GM2, in transplanted cells was greater than in cultured cells. Also, transplanted cells containing GalNAc-GM1b newly expressed GM1b, suggesting that the activity of GalNAc transferase for synthesis of GalNAc-GM1b is retarded on subcutaneous transplantation. The ganglioside composition, with GM3 as the major one, in the transplanted cells was similar to that in cervical carcinoma tissues, and thus, the expression of gangliosides with GM2-determinant seemed to be accelerated under cell-cultivation conditions.

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SV-40 transformation: effect on GM2 ganglioside in cultured cell lines

Cited by 7 publications

References 12 publications

Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

Enhanced expression of unique gangliosides with GM2-determinant in human uterine cervical carcinoma-derived cell lines

Cross-reactive antibodies against GM2 and CMV-infected fibroblasts in Guillain–Barré syndrome

Reduced expression of gangliosides with GM2-determinant in cervical carcinoma-derived cells after subcutaneous transplantation into nude mice

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