Sweroside promotes osteoblastic differentiation and mineralization via interaction of membrane estrogen receptor-α and GPR30 mediated p38 signalling pathway on MC3T3-E1 cells

Wu, Qing-chang; Tang, Xiyang; Dai, Zi-qin; Dai, Yi; Xiao, Huihui; Yao, Xin‐Sheng

doi:10.1016/j.phymed.2019.153146

Cited by 18 publications

(16 citation statements)

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“…confirmed 15 components including sweroside, epimedin B, isopsoralen, asperosaponin VI, and neobavaisoflavone, which came from four herbs - Longspur epimedium , Radix dipsaci , Rhizoma anemarrhenae , and Psoralea corylifolia - in the formula ( 34 ). Sweroside interacts with membrane estrogen receptor-α and p38 pathway to promote osteoblastic differentiation and mineralization ( 35 ). Epimedin B downregulates PI3K-Akt, MAPK, and PPAR signaling pathways, which are mentioned above to result in bone destruction in mice ( 36 ) and have estrogen-like effects ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

“…Some other compounds like isopsoralen, asperosaponin VI, and neobavaisoflavone have also been proved to either enhance osteogenesis or inhibit osteoclast activation ( 38 – 42 ). Moreover, Wu found that the combination of six typical absorbed constituents of XLGB capsule could promote MC3T3-E1 cells’ differentiation and mineralization, which were not seen in any single-constituent groups, suggesting that there may be some unknown interactive mechanism of the combination ( 12 ). Moreover, Ren et al., found that XLGB capsule downregulated RANKL mRNA and upregulated the mRNA of osteoprotegerin, which combines with RANKL to reduce osteoclasts, finally inhibiting bone destruction ( 11 ).…”

Section: Discussionmentioning

confidence: 99%

“…XLGB capsule was found to downregulate RANKL mRNA and upregulate osteoprotegerin mRNA, which combines with RANKL to reduce osteoclasts, finally inhibiting bone destruction ( 11 ). It was also reported that the combination of six typical absorbed constituents of XLGB capsule could promote MC3T3-E1 cells’ differentiation and mineralization ( 12 ). In TCM theory, most bone diseases pertain to the deficiency of the kidney and the blood stasis in meridians which causes pain, thus, this formula could nourish the liver and the kidney, promote blood circulation, and remove meridian obstruction, to strengthen the muscles and bones.…”

Section: Introductionmentioning

confidence: 99%

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Chinese Proprietary Medicine Xianling Gubao Capsule for Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

Cheng

Wu²,

Gao³

et al. 2022

Front. Endocrinol.

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ObjectiveTo assess the benefit and harm of Chinese medicine Xianling Gubao (XLGB) capsule compared to conventional medication or placebo to inform clinical practice.MethodsWe included randomized controlled trials (RCTs) with Jadad score ≥3 of XLGB capsule compared to pharmaceutical medication, placebo, or no treatment for primary osteoporosis. We conducted searches in EMBASE, Cochrane CENTRAL, MEDLINE, China National Knowledge Infrastructure, VIP, Wanfang, and Chinese Biomedical Literature Database (Sino-Med) from their inception till November 13th, 2021. Study selection and data extraction were done by two authors independently. The methodological quality of the RCTs was assessed using Cochrane’s risk of bias tool. The effect size was presented as risk ratio (RR) or mean difference (MD) with their 95% confidence interval (CI).ResultsOur searches identified 2292 records and after exclusions, eight trials involving 846 participants were included. There was no statistically significant difference between conventional medications with or without XLGB on new fracture (RR: 0.50, 95% CI: [0.13, 1.87]). Quality of life by SF-36 questionnaire of XLGB plus calcium carbonate, vitamin D3, and calcitriol was improved than that of without XLGB (MD: 6.72 scores, 95% CI: [2.82, 10.62]). XLGB increased bone mineral density similarly as calcium carbonate plus vitamin D3 (MD: 0.21, 95% CI: [-0.16, 0.58]) or as alendronate sodium, calcium carbonate plus vitamin D3 (MD: 0.00, 95% CI: [-0.10, 0.10]), but it had no additional effect as an add-on treatment to conventional medications (MD: 0.13, 95% CI: [-0.12, 0.37]). XLGB relieved pain via visual analog scale more effectively when combined with medications (MD: -1.55 score, 95% CI: [-2.47, -0.63]). XLGB as monotherapy did not increase adverse events (RR: 0.63, 95% CI: [0.28, 1.41]), or as an add-on treatment (RR: 0.25, 95% CI: [0.03, 2.16]).ConclusionThis systematic review shows that XLGB capsule appears to be safe and has a beneficial effect on the quality of life and pain relief when used alone or in combination with conventional medications in osteoporosis patients. Further large, rigorous trials are warranted to test its long-term benefit.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chinese Proprietary Medicine Xianling Gubao Capsule for Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

Cheng

Wu²,

Gao³

et al. 2022

Front. Endocrinol.

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“…However, each cross-reactive compound is biologically active. [29][30][31][32][33][34] Thus, each of these compounds impact the efficacy of S. japonica. The amounts of SM in the S. japonica samples usually exceeded the amounts of gentiopicroside and sweroside.…”

Section: Binding Selectivity Of Mab D2mentioning

confidence: 99%

A monoclonal antibody‐based indirect competitive enzyme‐linked immunosorbent assay to quantify swertiamarin and related compounds in Swertia japonica Makino

Nuntawong

Horikawa

Ochi

et al. 2020

Phytochemical Analysis

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Introduction: Swertia japonica Makino (S. japonica) has a long history of use as a folk medicine, and it is one of the three essential Japanese folk medicines. S.japonica has been reported to have various biological activities. The biologically active secoiridoid glycoside swertiamarin (SM) has been isolated from S. japonica. The efficacy of this plant is attributed to SM and related secoiridoid glycosides. To control the quality of S. japonica for medicinal use, a method for the determination of SM and other secoiridoid glycosides in the plant is needed.Objective: To produce an anti-SM monoclonal antibody (MAb) and develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) for S. japonica standardisation and quality control.Methodology: SM was conjugated to cationised bovine serum albumin (cBSA), and the SM-cBSA conjugate was used to immunise BALB/c mice. Splenocytes from the immunised mice were then fused with SP2/0 myeloma cells to produce hybridoma cells that expressed anti-SM MAb.Results: The developed icELISA was sufficiently sensitive and had a quantitative range of 0.78 to 12.5 μg/mL. Coefficients of variation below 10% indicated good repeatability. Recoveries in a spike and recovery assay ranged from 91.84% to 115.50%, which confirmed that the icELISA was accurate. The SM content measured using the icELISA was in agreement with the results of a high-performance liquid chromatography-ultraviolet (HPLC-UV) assay. Conclusion:The icELISA is suitable for the high-throughput analysis of SM and other secoiridoid glycosides in S. japonica. The method is fast, economical, and reliable for S. japonica quality control.

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“…As a membrane estrogen receptor, G protein-coupled receptor 30 (GPR30) is widely expressed in reproductive tissues, such as mammary glands [5], ovary [6], bone [7], uterus [8], and others [9,10]. After binding with estrogen, GPR30 mediates the rapid non-genomic actions of estrogen by stimulating the intracellular second messenger signals [11].…”

Section: Introductionmentioning

confidence: 99%

Activation of G Protein-coupled Receptor 30 Promotes Proliferation of Goat Mammary Epithelial Cells via MEK/Erk&PI3K/Akt Signaling Pathway

Zhao

Liu

Fan

et al. 2020

Preprint

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BackgroundGoat is an important dairy animal. During lactation, maintaining a high proliferative activity in goat mammary epithelial cells (GMECs) is significant to improve the yield and composition of goat milk. Estrogen is an essential hormone in epithelial cell proliferation and ductal morphogenesis of mammary gland. G protein-coupled receptor 30 (GPR30) is a novel membrane receptor of estrogen. However, the relationship between estrogen/GPR30 signaling and proliferation of goat mammary epithelial cells has not been reported. And the molecular mechanisms underlying the proliferative effect of estrogen via GPR30 on GMECs remain unclear.ResultsTo investigate the effect of estrogen/GPR30 signaling on GMECs proliferation, goat mammary epithelial cells, which expressed cytokeratin 18 and β-casein, were isolated and identified, defining their mammary alveolar epithelium origination. Estrogen and GPR30 agonist G1 obviously promoted the proliferation of GEMCs, in contrast, GPR30 antagonist G15 partly abolished estrogen-induced cell proliferation. Remarkably, the stimulatory effect of estrogen and G1 on GMECs growth was suppressed by GPR30 knockdown detected by cell counting assay, CCK-8 assay, and BrdU assay, suggesting that estrogen/GPR30 signaling was involved in GMECs proliferation. Additionally, G15 decreased cyclin D1, cyclin B1, CDK1, and p-CDK1 expression, resulting in cell cycle arrest in the G2/M phase via a down-regulated phosphorylation of Erk1/2 and Akt compared with estrogen alone. What’s more, knock-down GPR30 led to an accumulation in the G2/M phase and inhibition of cyclin D1, cyclin B1, CDK1, and p-CDK1 expression via a down-regulation of phosphorylated Erk1/2 and Akt despite the presence of estrogen and G1. Furthermore, MEK inhibitor and PI3K inhibitor decreased the expression of cyclin D1, cyclin B1, CDK1, and p-CDK1, and repressed estrogen-induced and G1-driven promotion of cell growth. It indicated that estrogen/GPR30 signaling played an important role in GMECs proliferation by affecting cell cycle progression via MEK/Erk&PI3K/Akt signaling pathway.ConclusionThis study may provide a new insight into the effect of estrogen/GPR30 signaling on the regulatory action of goat mammary gland development.

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Sweroside promotes osteoblastic differentiation and mineralization via interaction of membrane estrogen receptor-α and GPR30 mediated p38 signalling pathway on MC3T3-E1 cells

Cited by 18 publications

References 90 publications

Chinese Proprietary Medicine Xianling Gubao Capsule for Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

Chinese Proprietary Medicine Xianling Gubao Capsule for Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

A monoclonal antibody‐based indirect competitive enzyme‐linked immunosorbent assay to quantify swertiamarin and related compounds in Swertia japonica Makino

Activation of G Protein-coupled Receptor 30 Promotes Proliferation of Goat Mammary Epithelial Cells via MEK/Erk&PI3K/Akt Signaling Pathway

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Sweroside promotes osteoblastic differentiation and mineralization via interaction of membrane estrogen receptor-α and GPR30 mediated p38 signalling pathway on MC3T3-E1 cells

Cited by 18 publications

References 90 publications

Chinese Proprietary Medicine Xianling Gubao Capsule for Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

Chinese Proprietary Medicine Xianling Gubao Capsule for Osteoporosis: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

A monoclonal antibody‐based indirect competitive enzyme‐linked immunosorbent assay to quantify swertiamarin and related compounds in Swertia japonica Makino

Activation of G Protein-coupled Receptor 30 Promotes Proliferation of Goat Mammary Epithelial Cells via MEK/Erk&amp;PI3K/Akt Signaling Pathway

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Activation of G Protein-coupled Receptor 30 Promotes Proliferation of Goat Mammary Epithelial Cells via MEK/Erk&PI3K/Akt Signaling Pathway