Swimming speed alteration of larvae of Balanus Amphitrite as a behavioural end-point for laboratory toxicological bioassays

Faimali, Marco; Garaventa, Francesca; Piazza, Veronica; Greco, Giuliano; Corrà, Christian; Magillo, F.; Pittore, Massimiliano; Giacco, Elisabetta; Gallus, Lorenzo; Falugi, Carla; Tagliafierro, Grazia

doi:10.1007/s00227-005-0209-9

Cited by 80 publications

(51 citation statements)

References 73 publications

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“…In contrast, no changes were recorded following a 3-h and 24-h exposures to copper at 0.01 mg ml -1 (Shimizu et al, 2002) and 5 mg l -1 (Untersteiner et al, 2003), and an increase in www.intechopen.com swimming speed occurred after a 24-h exposure to methyl-paraoxon at 0.7 mg l -1 (Duquesne & Küster, 2010). Similar results were obtained on the larvae of the cirriped Balanus Amphitrite (Faimali et al, 2006), the crustacean Artemia sp. and the rotifer Brachionus plicatilis (Garaventa et al, 2010) exposed to a range of chemical pollutants (i.e.…”

Section:  supporting

confidence: 62%

“…As a consequence, and even if alterations in swimming speed have been detected at toxic compound concentrations well below LC50 values for a range of invertebrates (Avila et al, 2010;Ihara et al, 2010;Garaventa et al, 2010;Cailleaud et al, 2011;Seuront, 2011a, present work), its claimed use as a non-specifc behavioral end-point in marine ecotoxicology and environmental monitoring program (Faimali et al, 2006;Garaventa et al, 2010) cannot be warranted. A practical alternative based on the intrinsic fractal nature of behavioral properties and their subsequent modification under stressful conditions, applied in both invertebrate (Seuront & Leterme, 2007;Seuront, 2010bSeuront, , 2011b and vertebrate (Escós et al, 1995;Alados et al, 1996;Alados & Huffman, 2000;María et al, 2004;Seuront & Cribb, 2011), including humans (e.g.…”

Section:  mentioning

confidence: 79%

“…As such, it is stressed that even if alterations in swimming speed have been detected at toxic compound concentrations well below LC50 values for a range of invertebrates (e.g. Avila et al, 2010;Ihara et al, 2010;Garaventa et al, 2010;Cailleaud et al, 2011;Seuront, 2011a), its claimed use as a non-specifc behavioral end-point in marine ecotoxicology and environmental monitoring program (Faimali et al, 2006;Garaventa et al, 2010) cannot be warranted.…”

Section: Resultsmentioning

confidence: 99%

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Hydrocarbon Contamination and the Swimming Behavior of the Estuarine Copepod Eurytemora affinis

Seuront¹

2012

Marine Ecosystems

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Section:  supporting

confidence: 62%

Section:  mentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

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Hydrocarbon Contamination and the Swimming Behavior of the Estuarine Copepod Eurytemora affinis

Seuront¹

2012

Marine Ecosystems

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“…Organisms are simultaneously identified and motion vectors used to calculate parameters that can be taken as biological end-points. Studies of locomotor behaviour changes, as a response to stress, have been performed on several aquatic vertebrates (Brachydanio rerio, Oncorhynchus mykiss, Gambusia holbrooki, Dicentrarchus labrax), (Kane et al 2004;Little and Finger 1990;Beauvais et al 2000;Vogl et al 1999, Gerhardt et al 2005, Georgalas et al 2007, invertebrates (Artemia salina, Balanus amphitrite, Brachionus calyciflorus, Daphnia magna, Hippolyte inermis), (Venkateswara Rao et al 2007;Amsler et al 2006;Faimali et al 2002Faimali et al , 2006Charoy and Janssen 1999;Charoy et al 1995;Goto and Hiromi 2003;Janssen et al 1994;Baillieul and Blust 1999;Shimizu et al 2002;Gerhardt et al 2005;Untersteiner et al 2003Untersteiner et al , 2005, and protists (Euglena gracilis) (Tahedl and Häder 2001).…”

Section: Introductionmentioning

confidence: 99%

Swimming speed alteration of Artemia sp. and Brachionus plicatilis as a sub-lethal behavioural end-point for ecotoxicological surveys

et al. 2010

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In this study, we investigated the possibility to improve a new behavioural bioassay (Swimming Speed Alteration test-SSA test) using larvae of marine cyst-forming organisms: e.g. the brine shrimp Artemia sp. and the rotifer Brachionus plicatilis. Swimming speed was investigated as a behavioural end-point for application in ecotoxicology studies. A first experiment to analyse the linear swimming speed of the two organisms was performed to verify the applicability of the video-camera tracking system, here referred to as Swimming Behavioural Recorder (SBR). A second experiment was performed, exposing organisms to different toxic compounds (zinc pyrithione, Macrotrol MT-200, and Eserine). Swimming speed alteration was analyzed together with mortality. The results of the first experiment indicate that SBR is a suitable tool to detect linear swimming speed of the two organisms, since the values have been obtained in accordance with other studies using the same organisms (3.05 mm s(-1) for Artemia sp. and 0.62 mm s(-1) for B. plicatilis). Toxicity test results clearly indicate that swimming speed of Artemia sp. and B. plicatilis is a valid behavioural end-point to detect stress at sub-lethal toxic substance concentrations. Indeed, alterations in swimming speed have been detected at toxic compound concentrations as low as less then 0.1-5% of their LC(50) values. In conclusion, the SSA test with B. plicatilis and Artemia sp. can be a good behavioural integrated output for application in marine ecotoxicology and environmental monitoring programs.

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“…The repellent function of non-toxic antifoulants can be defined as a negative motor response, taxis or kinesis movement in response to stimuli that drive organisms away from the source of the stimulus (Railkin 2004) and thus, can be determined using locomotor activity, phototaxis, or settlement bioassays (Wu et al 1997a(Wu et al , 1997bAmsler et al 2006;Faimali et al 2006;. However, behavioral bioassays offer very little information about the molecular mechanisms of AF compounds since it is almost impossible to link the changes in behavior with changes in the genes, proteins, or pathways in the target organisms.…”

Section: Behavioral Responsesmentioning

confidence: 99%

Mini-review: Molecular mechanisms of antifouling compounds

2013

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Various antifouling (AF) coatings have been developed to protect submerged surfaces by deterring the settlement of the colonizing stages of fouling organisms. A review of the literature shows that effective AF compounds with specific targets are ones often considered non-toxic. Such compounds act variously on ion channels, quorum sensing systems, neurotransmitters, production/release of adhesive, and specific enzymes that regulate energy production or primary metabolism. In contrast, AF compounds with general targets may or may not act through toxic mechanisms. These compounds affect a variety of biological activities including algal photosynthesis, energy production, stress responses, genotoxic damage, immunosuppressed protein expression, oxidation, neurotransmission, surface chemistry, the formation of biofilms, and adhesive production/release. Among all the targets, adhesive production/release is the most common, possibly due to a more extensive research effort in this area. Overall, the specific molecular targets and the molecular mechanisms of most AF compounds have not been identified. Thus, the information available is insufficient to draw firm conclusions about the types of molecular targets to be used as sensitive biomarkers for future design and screening of compounds with AF potential. In this review, the relevant advantages and disadvantages of the molecular tools available for studying the molecular targets of AF compounds are highlighted briefly and the molecular mechanisms of the AF compounds, which are largely a source of speculation in the literature, are discussed.

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Swimming speed alteration of larvae of Balanus Amphitrite as a behavioural end-point for laboratory toxicological bioassays

Cited by 80 publications

References 73 publications

Hydrocarbon Contamination and the Swimming Behavior of the Estuarine Copepod Eurytemora affinis

Hydrocarbon Contamination and the Swimming Behavior of the Estuarine Copepod Eurytemora affinis

Swimming speed alteration of Artemia sp. and Brachionus plicatilis as a sub-lethal behavioural end-point for ecotoxicological surveys

Mini-review: Molecular mechanisms of antifouling compounds

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