Switching Rho GTPase activation into effective antibacterial defenses requires the caspase-1/IL-1beta signaling axis

Boyer, Laurent; Lemichez, Emmanuel

doi:10.1080/21541248.2015.1095698

Cited by 4 publications

(4 citation statements)

References 34 publications

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“…Three specific genes (i.e., hlyCA, cnf, sfa/focDE ) were found in the five most IL-1β-inducible strains. Many previous studies have shown the important role of hemolysin and CNF in IL-1β secretion by macrophages upon infection via pathways that include activation of Rho GTPase, NF-κB, inflammasomes, and pyroptosis 20,45–48 . Hemolysin encoded by various organisms is well known to activate NLRP3 inflammasome to produce the active form of IL-1β 49 .…”

Section: Discussionmentioning

confidence: 99%

Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

Jung

Hong

Gharderpour

et al. 2019

Sci Rep

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Urinary tract infection (UTI) is one of the most common bacterial infections in infants less than age 1 year. UTIs frequently recur and result in long-term effects include sepsis and renal scarring. Uropathogenic Escherichia coli (UPEC), the most prevalent organism found in UTIs, can cause host inflammation via various virulence factors including hemolysin and cytotoxic necrotizing factors by inducing inflammatory cytokines such as interleukin (IL)-1β. However, the ability of each UPEC organism to induce IL-1β production may differ by strain. Furthermore, the correlation between differential IL-1β induction and its relevance in pathology has not been well studied. In this study, we isolated UPEC from children under age 24 months and infected bone-marrow derived macrophages with the isolates to investigate secretion of IL-1β. We found that children with higher concentrations of C-reactive protein (CRP) were more likely to harbor phylotype B2 UPEC strains that induced more IL-1β production than phylotype D. We also observed a significant correlation between serum CRP level and in vitro IL-1β induction by phylotype B2 UPEC bacteria. Our results highlight the diversity of UPEC in terms of IL-1β induction capacity in macrophages and suggest a potential pathogenic role in UTIs by inducing inflammation in infants.

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Section: Discussionmentioning

confidence: 99%

Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

Jung

Hong

Gharderpour

et al. 2019

Sci Rep

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“…Bacteria overcome host defenses, by hijacking Rho GTPases that regulate the actin cytoskeleton [ 11 , 12 , 51 ]. During initiation of infection, bacterial adhesins favor tissue colonization, whereas, at later stages, exotoxins promote bacterial spread and blockage of immune cell responses [ 52 ].…”

Section: Methodsmentioning

confidence: 99%

Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases

Simons

Bondu

Wandinger‐Ness

et al. 2018

Methods in Molecular Biology

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Small, monomeric guanine triphosphate hydrolases (GTPases) are ubiquitous cellular integrators of signaling. A signal activates the GTPase, which then binds to an effector molecule to relay a signal inside the cell. The GTPase effector trap flow cytometry assay (G-Trap) utilizes bead-based protein immobilization and dual-color flow cytometry to rapidly and quantitatively measure GTPase activity status in cell or tissue lysates. Beginning with commercial cytoplex bead sets that are color-coded with graded fluorescence intensities of a red (700 nm) wavelength, the bead sets are derivatized to display glutathione on the surface through a detailed protocol described here. A different glutathione-S-transferase-effector protein (GST-effector protein) can then be attached to the surface of each set. For the assay, users can incubate bead sets individually or in a multiplex format with lysates for rapid, selective capture of active, GTP-bound GTPases from a single sample. After that, flow cytometry is used to identify the bead-borne GTPase based on red bead intensity, and the amount of active GTPase per bead is detected using monoclonal antibodies conjugated to a green fluorophore or via labeled secondary antibodies. Three examples are provided to illustrate the efficacy of the effector-functionalized beads for measuring the activation of at least five GTPases in a single lysate from fewer than 50,000 cells.

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“…Cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli , although not a classical type III secreted effector, destroys the GTPase activity of Rac2, RhoA and Cdc42 by deamidating certain glutamine residues, thereby rendering these GTPases permanently activated. CNF1 presence, however, also induces inflammatory reactions like nuclear factor-κB (NF-κB) activation and cytokine secretion, through the innate immune adaptors, IMD in Drosophila and RIP2 in mammals [ 107 , 108 ]. Similar inflammatory reactions are also triggered by the abovementioned Salmonella effector protein SopE [ 109 , 110 ].…”

Section: An Excursion Into Mammalian Rho Signaling In Bacterial Pamentioning

confidence: 99%

Regulation and Functions of ROP GTPases in Plant–Microbe Interactions

Engelhardt

Trutzenberg

Hückelhoven

2020

Cells

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Rho proteins of plants (ROPs) form a specific clade of Rho GTPases, which are involved in either plant immunity or susceptibility to diseases. They are intensively studied in grass host plants, in which ROPs are signaling hubs downstream of both cell surface immune receptor kinases and intracellular nucleotide-binding leucine-rich repeat receptors, which activate major branches of plant immune signaling. Additionally, invasive fungal pathogens may co-opt the function of ROPs for manipulation of the cytoskeleton, cell invasion and host cell developmental reprogramming, which promote pathogenic colonization. Strikingly, mammalian bacterial pathogens also initiate both effector-triggered susceptibility for cell invasion and effector-triggered immunity via Rho GTPases. In this review, we summarize central concepts of Rho signaling in disease and immunity of plants and briefly compare them to important findings in the mammalian research field. We focus on Rho activation, downstream signaling and cellular reorganization under control of Rho proteins involved in disease progression and pathogen resistance.

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Switching Rho GTPase activation into effective antibacterial defenses requires the caspase-1/IL-1beta signaling axis

Cited by 4 publications

References 34 publications

Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

Small-Volume Flow Cytometry-Based Multiplex Analysis of the Activity of Small GTPases

Regulation and Functions of ROP GTPases in Plant–Microbe Interactions

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