Symbiotic effectiveness and bacteriocin production by Rhizobium leguminosarum bv. viciae isolated from agriculture soils in Faisalabad

Hafeez, Fauzia Yusuf; Naeem, Farrukh; Naeem, Rehan; Zaidi, Arsalan; Malik, Kausar

doi:10.1016/j.envexpbot.2004.06.008

Cited by 39 publications

(30 citation statements)

References 16 publications

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“…Similarly, Hassan et al (2004) evidenced that the response upon mixing the strains was not always as good as the response to a single strain on inoculants. Hafeez et al (2005) suggested that the antagonistic effect may be due to the production of bacteriocin, proteins or protein complexes with bacteriocidal activity directed against species closely related to the producer bacterium. The differential response showed among the inoculated strains alone, in the fi rst experiment, compared to the second one, coincided with previous results that pointed out unstable behavior of inoculated strains in different environments (Mostasso et al, 2002;Hafeez et al, 2005;Soares et al, 2006).…”

Section: Af3648391mentioning

confidence: 99%

“…Unfortunately, biological nitrogen fi xation in common bean fi eld crops has exhibited unstable behavior (Mostasso et al, 2002;Hafeez et al, 2005;Soares et al, 2006). Several approaches have been used to obtain new rhizobia strains which exhibit better biological nitrogen fi xation responses on common bean.…”

Section: Introductionmentioning

confidence: 99%

“…Several approaches have been used to obtain new rhizobia strains which exhibit better biological nitrogen fi xation responses on common bean. Typically, these strains are evaluated individually Soares et al, 2006) or in mixture with other strains (Hassan et al, 2004;Hafeez et al, 2005;Raposeiras et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

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-This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fi xation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the fi rst experiment, the nodulation and nitrogen fi xation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes fi lled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. In the second experiment, the nodulation and productivity of common beans Perola cultivar were determined, with the use of evaluated strains, alone or in mixture with PRF-81 strain. It was carried out in pots fi lled with soil. The native soil population was identifi ed as Rhizobium sp. and was ineffi cient in nitrogen fi xation. Three different Rhizobium species were found among the four new strains. The LBMP-4BR and LBMP-12BR new strains are among the ones with greatest nodulation and fi xation capacity and exhibit differential responses when mixed to PRF-81.Index terms: Phaseolus vulgaris, biological nitrogen fi xation, intergenic space, phylogeny, sequencing, soil rhizobia population. Caracterização genética e capacidade de fi xação de nitrogênio de estirpes de Rhizobium em feijoeiroResumo -O objetivo deste trabalho foi a caracterização genética de quatro novas estirpes de Rhizobium e a avaliação de sua capacidade de fi xação de N 2 e nodulação, comparadas a estirpes comerciais e à população nativa de rizóbios de um Latossolo Vermelho. Dois experimentos foram conduzidos em blocos ao acaso, em casa de vegetação. No primeiro experimento, conduzido em tubetes com vermiculita, avaliaram-se a nodulação e a capacidade de fi xação das novas estirpes, em comparação com as estirpes comerciais CIAT-899 e PRF-81 e com a população nativa do solo. Das colônias puras isoladas, extraiu-se o DNA genômico e realizou-se o seqüenciamento do espaço intergênico, para a caracterização genética das estirpes e da população nativa de rizóbios. O segundo experimento foi realizado em vasos com solo, para determinação da produtividade e da nodulação do feijoeiro, cultivar Pérola, com o uso das estirpes isoladamente ou em mistura com a PRF-81. A população nativa do solo foi identifi cada como Rhizobium sp. e se mostrou inefi ciente na fi xação de nitrogênio. Foram encontradas três espécies de Rhizobium entre as quatro novas estirpes. As estirpes LBMP-4BR e LBMP-12BR estão entre as que têm maior capacidade de nodulação e fi xação de N 2 , e apresentam respostas diferenciadas quando misturadas à PRF-81.Termos para indexação: Phaseolus vulgaris, fi xação biológica de nitrogênio, espaço intergênico, fi logenia, seqüenciamento, população nativa de rizóbios.

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Section: Af3648391mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

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“…This is consistent with the earlier report that the strains of Rhizobium leguminosarum bv. viciae differ in their activity spectrum 27 .…”

Section: Resultsmentioning

confidence: 99%

Production Bacteriosin by Rhizobia obtained from Root nodules of Macrotyloma uniflorum (Lam.) Verdc. (Horse Gram)

Edulamudi

Johnson

Divi

et al. 2012

Bangla. J. Microbiol.

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Thirty two Rhizobium isolates obtained from the root nodules of Horse gram plants grown in 32 different soil samples collected from various regions in Andhra Pradesh, India. They were identified as rhizobia by nodulation test. These isolates were used to study the production of bacteriocins. All the isolates produced bacteriocins against the remaining isolates. The isolates HGR-4 and 9 showed maximum activity/inhibition zone among all the isolates, and also found to be inhibiting more number of isolates than that of other bacteriocin producing isolates. The bacteriocin protein substance produced by the isolates HGR-4 and 9 showed a molecular weight of ~ 50 kDa by SDS-AGE and bacteriocin plasmids molecular weight is > 22 kb and > 24 kb.

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“…Biofertilization technology is practiced for increasing agricultural production, to limit the use of chemical fertilizers and pollution of environment through seed inoculation by different types of bacteria (Abd El-Ghany, 1994). Rhizobium colonization in non-legumes such as wheat, Brassica and Arabidopsis thaliana does not require the Rhizobium nodulation genes which are involved in the infection and nodulation of legume roots (Hafeez et al, 2005). Efforts at extending N 2 -fixing ability to important non-leguminous crops such as cereals have long been a major goal of researchers in the field of biological nitrogen fixation.…”

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confidence: 99%

Effect of Magnetic Iron and Biofertilization Treatments on Wheat (Triticum aestivum L.) Productivity under Salinity Conditions

2014

Egypt. J. Agron.

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WO FIELD experiments were conducted at Ras-Sudr Research ….. Station, South Sinai Governorate during two successive seasons, 2011/2012 and 2012/2013 to study the effect of four levels of magnetic iron (0, 50, 100 and 150 kg /fed) and four biofertilization; control, Pseudomonas fluorescence and Rhizobium meliloti individual and mixed application on the yield parameters of wheat (Triticum aestivum L., cv. Sakha 94). Results revealed that increasing of the magnetic iron from 50 up to 150 kg /fed significantly increased all yield and yield components parameters; plant height (cm), spike length (cm), number of spikelets / spike, weight of 1000 grains (g), biological yield (kg /fed), grain yield (kg /fed) and straw yield (kg/fed) compared with the control in each growing seasons. In single inoculation Rizobium gave better result than other treatments for all yield criteria in the two growing seasons. The effect of the interaction indicates that inoculating of the Rizobium, fertilized with 100 kg magnetic iron /fed resulted in the highest yield and yield components in the two growing seasons. Interaction resulted in between magnetic iron and biofertilization resulted in higher grain protein than the control. In addition, the percentage of K, K/Na ratio, N, and grain protein of the Sakha 94 significantly increased in the two growing seasons. The analysis of the data collected during the study proved that there were statistically significant increases of wheat yield and yield components and some chemical contents due to different rates of magnetic treatments compared by the control which revealed decreases in the previously mentioned characters. Generally, the best treatments were 100 kg magnetic iron /fed and Rizobium meliloti. Both treatments were more effective in avoiding the adverse effects of soil salinity on wheat productivity compered to other treatments used.

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Symbiotic effectiveness and bacteriocin production by Rhizobium leguminosarum bv. viciae isolated from agriculture soils in Faisalabad

Cited by 39 publications

References 16 publications

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Production Bacteriosin by Rhizobia obtained from Root nodules of Macrotyloma uniflorum (Lam.) Verdc. (Horse Gram)

Effect of Magnetic Iron and Biofertilization Treatments on Wheat (Triticum aestivum L.) Productivity under Salinity Conditions

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