Synbiotic VSL#3 and yacon-based product modulate the intestinal microbiota and prevent the development of pre-neoplastic lesions in a colorectal carcinogenesis model

Cruz, Bruna Cristina dos Santos; Duarte, Viní­cius da Silva; Giacomini, Alessio; Corich, Viviana; Paula, Sérgio Oliveira de; Fialho, Lílian da Silva; Guimarães, Valéria Monteze; Ferreira, Célia Lúcia de Luces Fortes; Pelúzio, Maria do Carmo Gouveia

doi:10.1007/s00253-020-10863-x

Cited by 28 publications

(30 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inhibition of histone deacetylase by butyrate increases mucus synthesis and mucosal thickness, as well as stimulates mucosal repair (Eslami et al, 2019;Molska & Regula, 2019). As previously demonstrated (Cruz et al, 2020a), the use of the synbiotic VSL#3 + PBY significantly increased SCFA concentrations, which may explain the reduction in intestinal permeability.…”

Section: 24supporting

confidence: 65%

“…According to the study's authors, the fermentation of FOS and inulin present in PBY leads to the production of metabolites that stimulate the proliferation of healthy cells, such as butyrate, which is the main source of energy for colonocytes (De Nadai Marcon et al, 2019). In fact, we previously demonstrated the significant increase in SCFA and butyrate production in the group that received the synbiotic VSL#3 + PBY (Cruz et al, 2020a).…”

Section: Body Weight Food Intake and Anatomical Characteristicsmentioning

confidence: 89%

“…In the present study, we did not observe differences in the number of areas with inflammatory infiltrate between the groups; however, we previously demonstrated that the use of the synbiotic VSL#3 + PBY was able to reduce TNF concentrations (1.4-fold, p = 0.028) and increase interleukins 2 (1.5-fold, p = 0.027) and 4 (1.5-fold, p = 0.035), while the probiotic VSL#3 increased interleukin 4 levels (1.6-fold, p = 0.044) (Cruz et al, 2020a). Furthermore, the CON group presented deeper infiltrate areas, reaching the submucosal and muscularis layers (Figure 3c).…”

Section: Colonic Morphometry and Histopathological Scorementioning

confidence: 92%

“…After a week of acclimatization, with free access to commercial diet (Purina R ) and water, animals were divided according to body weight into three different groups (n = 15/group), to receive the following interventions, for 13 weeks, as previously described by Cruz et al (2020a) 3. Synbiotic group (SYN): modified AIN-93 M diet, with PBY (6% FOS and inulin) and 0.1 ml of probiotic VSL#3 (2.25 × 10 9 CFU/animal), via orogastric gavage.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of the efficacy of probiotic VSL#3 and synbiotic VSL#3 and yacon‐based product in reducing oxidative stress and intestinal permeability in mice induced to colorectal carcinogenesis

Cruz

Moraes

Marcon

et al. 2021

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

The objective of the present study was to evaluate the effect of probiotic VSL#3 isolated or associated with a yacon-based product (synbiotic) on oxidative stress modulation and intestinal permeability in an experimental model of colorectal carcinogenesis. Forty-five C57BL/6J mice were divided into three groups: control (standard diet AIN-93 M); probiotic (standard diet AIN-93 M and multispecies probiotic VSL#3, 2.25 × 10 9 CFU), and synbiotic (standard diet AIN-93 M with yacon-based product, 6% fructooligosaccharides and inulin, and probiotic VSL#3, 2.25 × 10 9 CFU). The experimental diets were provided for 13 weeks. The probiotic and the yacon-based product showed antioxidant activity, with the percentage of DPPH radical scavenging equal to 69.7 ± 0.4% and 74.3 ± 0.1%, respectively. These findings contributed to reduce hepatic oxidative stress: the control group showed higher concentration of malondialdehyde (1.8fold, p = 0.007 and 1.5-fold, p = 0.035) and carbonylated protein (2-fold, p = 0.008 and 5.6-fold, p = 0.000) compared to the probiotic and synbiotic groups, respectively. Catalase enzyme activity increased 1.43-fold (p = 0.014) in synbiotic group.The crypt depth increased 1.2-fold and 1.4-fold with the use of probiotic and synbiotic, respectively, compared to the control diet (p = 0.000). These findings corroborate the reduction in intestinal permeability in the probiotic and synbiotic groups, as measured by the percentage of urinary lactulose excretion (CON: 0.93 ± 0.62% × PRO: 0.44 ± 0.05%, p = 0.048; and CON: 0.93 ± 0.62% × SYN: 0.41 ± 0.12%, p = 0.043). In conclusion, the probiotic and synbiotic showed antioxidant activity, which contributed to the reduction of oxidative stress markers. In addition, they protected the mucosa from damage caused by chemical carcinogen and reduced intestinal permeability. Practical Application:The relationship between intestinal health and the occurrence of various organic disorders has been demonstrated in many

show abstract

Section: 24supporting

confidence: 65%

Section: Body Weight Food Intake and Anatomical Characteristicsmentioning

confidence: 89%

Section: Colonic Morphometry and Histopathological Scorementioning

confidence: 92%

Section: Animals and Experimental Designmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the efficacy of probiotic VSL#3 and synbiotic VSL#3 and yacon‐based product in reducing oxidative stress and intestinal permeability in mice induced to colorectal carcinogenesis

Cruz

Moraes

Marcon

et al. 2021

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…In Wistar rats with induced CRC, Yacón flour as a source of fructooligosaccharides may help to maintain the integrity of their intestinal health [ 25 ]. Similarly, this prebiotic, along with the commercial probiotic VSL#3 ® (Sigma-Tau Pharmaceuticals, Gaithersburg, MD, USA) showed extra benefits in C57BL6/J mice, in comparison with the use of VSL#3 ® alone, culminating in a significant reduction in precursor lesions of CRC [ 26 ]. In other new researches on rats developing CRC, either genetically or by induction carcinogens such as AOM/DSS, various prebiotics such as galacto-oligosaccharides derived from lactulose [ 27 ], inulin [ 28 ], phenolic compounds such as anthocyanins and ellagic acid from Myrciaria jaboticaba ((Vell.)…”

Section: Prebiotics and Crcmentioning

confidence: 99%

Preventing Colorectal Cancer through Prebiotics

2021

View full text Add to dashboard Cite

Colorectal cancer (CRC), the third most common cancer in the world, has been recently rising in emerging countries due to environmental and lifestyle factors. Many of these factors are brought up by industrialization, which includes lack of physical activity, poor diet, circadian rhythm disruption, and increase in alcohol consumption. They can increase the risk of CRC by changing the colonic environment and by altering gut microbiota composition, a state referred to as gut dysbiosis. Prebiotics, which are nutrients that can help maintain intestinal microbial homeostasis and mitigate dysbiosis, could be beneficial in preventing inflammation and CRC. These nutrients can hinder the effects of dysbiosis by encouraging the growth of beneficial bacteria involved in short-chain fatty acids (SCFA) production, anti-inflammatory immunity, maintenance of the intestinal epithelial barrier, pro-apoptotic mechanisms, and other cellular mechanisms. This review aims to summarize recent reports about the implication of prebiotics, and probable mechanisms, in the prevention and treatment of CRC. Various experimental studies, specifically in gut microbiome, have effectively demonstrated the protective effect of prebiotics in the progress of CRC. Hence, comprehensive knowledge is urgent to understand the clinical applications of prebiotics in the prevention or treatment of CRC.

show abstract

Bioactive Compounds and Biological Activities of Yacon Root (Smallanthus sonchifolius) on Gut-Liver-Adipose Tissue Axis

Romualdo,

de Souza,

da Rocha Viana

et al. 2023

Reference Series in Phytochemistry

View full text Add to dashboard Cite

Synbiotic VSL#3 and yacon-based product modulate the intestinal microbiota and prevent the development of pre-neoplastic lesions in a colorectal carcinogenesis model

Cited by 28 publications

References 96 publications

Evaluation of the efficacy of probiotic VSL#3 and synbiotic VSL#3 and yacon‐based product in reducing oxidative stress and intestinal permeability in mice induced to colorectal carcinogenesis

Evaluation of the efficacy of probiotic VSL#3 and synbiotic VSL#3 and yacon‐based product in reducing oxidative stress and intestinal permeability in mice induced to colorectal carcinogenesis

Preventing Colorectal Cancer through Prebiotics

Bioactive Compounds and Biological Activities of Yacon Root (Smallanthus sonchifolius) on Gut-Liver-Adipose Tissue Axis

Contact Info

Product

Resources

About