Synbiotics in caries prevention: A scoping review

Bijle, Mohammed Nadeem Ahmed; Ekambaram, Manikandan; Lo, Edward Chin Man; Yiu, Cynthia Kar Yung

doi:10.1371/journal.pone.0237547

Cited by 18 publications

(10 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As per WHO, probiotics are defined as "live micro-organisms which when administered in adequate amounts confer a health benefit on the host". 11 Several invitro studies examining the effect of probiotics on caries prevention have been conducted in the last two decades using different probiotics strains and species; however, probiotic colonization in the oral cavity is transient. Therefore, formulations enhancing probiotics survival are needed to impart long-standing cariespreventive benefits.…”

Section: Discussionmentioning

confidence: 99%

Probiotic Streptococcus A12 Strain in Caries Prevention: A Systematic Review

Qazi¹,

Pawar²,

Tharakan³

et al. 2021

IJPHRD

View full text Add to dashboard Cite

Background: Dental Caries is a biofilm-mediated multi factorial disease affecting around 5 billion people worldwide. A consensus is now emerging that caries preventive measures should not only correct the environmental pressures responsible for the plaque biofilm dysbiosis, but also help to maintain a healthy resident microbiome which plays a crucial role in preventing caries and promoting oral health. This PROSPERO registered review (registration no 166695) aimed to examine the evidence on the role of probiotic Streptococcus A12 in caries prevention.Objectives: To know the effectiveness of A12 strain in caries prevention in early stages of disease onset. Intervention: Streptococcus A12 strains.Results: A preliminary search yielded a total of 2630 studies recorded through database searching and 23 additional records were identified through manual search. From 2653 articles, 2504 were excluded based on screening through review of titles. 8 articles were included for qualitative synthesis. Streptococcus A12 strains demonstrates antimicrobial efficacy by neutralization of the environment by arginine metabolism. Conclusion:Streptococcus A12 strains demonstrates antimicrobial efficacy by neutralization of the environment by arginine metabolism, which leads to the reduction in Streptococcus mutans count and helps in lowering the acidic nature of saliva is the potential strength of A12 strain. Due to a lack of controlled clinical studies on probiotics for caries prevention, evidence on their caries-preventive potential is weak. Future studies are needed to examine the probiotics as a potential agent against cariogenic pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Probiotic Streptococcus A12 Strain in Caries Prevention: A Systematic Review

Qazi¹,

Pawar²,

Tharakan³

et al. 2021

IJPHRD

View full text Add to dashboard Cite

show abstract

“…Since lactobacilli are often considered late colonizers of the pellicle, probiotics alone are insufficient to prevent cavities [10,11]. To accelerate the use of conventional probiotics, which have well-characterized and well-documented health benefits, an adjuvant for that promotes the colonization of probiotics while modulating the virulence factor of the pathogen is necessary [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…Prebiotics impact the functional potential of bacterial cells [15,16]. Therefore, probiotics are in a combination of prebiotics (referred to as synbiotics), that synergically modulate the virulence of S. mutans and, in turn, inhibit cariogenic biofilm formation [17].…”

Section: Introductionmentioning

confidence: 99%

Collagen Peptide in a Combinatorial Treatment with Lactobacillus rhamnosus Inhibits the Cariogenic Properties of Streptococcus mutans: An In Vitro Study

Jung

Cai

Yoo³

et al. 2022

IJMS

View full text Add to dashboard Cite

Dental caries is caused by the formation of cariogenic biofilm, leading to localized areas of enamel demineralization. Streptococcus mutans, a cariogenic pathogen, has long been considered as a microbial etiology of dental caries. We hypothesized that an antagonistic approach using a prebiotic collagen peptide in combination with probiotic Lactobacillus rhamnosus would modulate the virulence of this cariogenic biofilm. In vitro S. mutans biofilms were formed on saliva-coated hydroxyapatite discs, and the inhibitory effect of a combination of L. rhamnosus and collagen peptide on S. mutans biofilms were evaluated using microbiological, biochemical, confocal imaging, and transcriptomic analyses. The combination of L. rhamnosus with collagen peptide altered acid production by S. mutans, significantly increasing culture pH at an early stage of biofilm formation. Moreover, the 3D architecture of the S. mutans biofilm was greatly compromised when it was in the presence of L. rhamnosus with collagen peptide, resulting in a significant reduction in exopolysaccharide with unstructured and mixed bacterial organization. The presence of L. rhamnosus with collagen peptide modulated the virulence potential of S. mutans via down-regulation of eno, ldh, and atpD corresponding to acid production and proton transportation, whereas aguD associated with alkali production was up-regulated. Gly-Pro-Hyp, a common tripeptide unit of collagen, consistently modulated the cariogenic potential of S. mutans by inhibiting acid production, similar to the bioactivity of a collagen peptide. It also enhanced the relative abundance of commensal streptococci (S. oralis) in a mixed-species biofilm by inhibiting S. mutans colonization and dome-like microcolony formation. This work demonstrates that food-derived synbiotics may offer a useful means of disrupting cariogenic communities and maintaining microbial homeostasis.

show abstract

“…Biotic strategies-prebiotics, probiotics, and synbiotics-are being explored as supplementary interventions to the long-known effects of fluorides (F) on dental caries [7]. Topical F application has led to a dramatic decline in dental caries worldwide [8,9]; however, its antimicrobial effect on cariogenic biofilms is limited [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Topical F application has led to a dramatic decline in dental caries worldwide [8,9]; however, its antimicrobial effect on cariogenic biofilms is limited [10,11]. The caries-preventive potential of synbiotics is still in the pre-clinical investigation stages [7], while probiotics are effective on pathogenic biofilms [12][13][14], with brief sustainability in the oral cavity [13,14]. The role of prebiotics in caries prevention (through clinical trials) has been explored over the past two decades, with reported superior caries-preventive potential than fluoride [15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Concentration-Dependent Multi-Potentiality of L-Arginine: Antimicrobial Effect, Hydroxyapatite Stability, and MMPs Inhibition

et al. 2021

Self Cite

View full text Add to dashboard Cite

This study’s objective was to examine L-arginine (L-arg) supplementation’s effect on mono-species biofilm (Streptococcus mutans/Streptococcus sanguinis) growth and underlying enamel substrates. The experimental groups were 1%, 2%, and 4% arg, and 0.9% NaCl was used as the vehicle control. Sterilised enamel blocks were subjected to 7-day treatment with test solutions and S. mutans/S. sanguinis inoculum in BHI. Post-treatment, the treated biofilms stained for live/dead bacterial cells were analysed using confocal microscopy. The enamel specimens were analysed using X-ray diffraction crystallography (XRD), Raman spectroscopy (RS), and transmission electron microscopy (TEM). The molecular interactions between arg and MMP-2/MMP-9 were determined by computational molecular docking and MMP assays. With increasing arg concentrations, bacterial survival significantly decreased (p < 0.05). The XRD peak intensity with 1%/2% arg was significantly higher than with 4% arg and the control (p < 0.05). The bands associated with the mineral phase by RS were significantly accentuated in the 1%/2% arg specimens compared to in other groups (p < 0.05). The TEM analysis revealed that 4% arg exhibited an ill-defined shape of enamel crystals. Docking of arg molecules to MMPs appears feasible, with arg inhibiting MMP-2/MMP-9 (p < 0.05). L-arginine supplementation has an antimicrobial effect on mono-species biofilm. L-arginine treatment at lower (1%/2%) concentrations exhibits enamel hydroxyapatite stability, while the molecule has the potential to inhibit MMP-2/MMP-9.

show abstract

Synbiotics in caries prevention: A scoping review

Cited by 18 publications

References 58 publications

Probiotic Streptococcus A12 Strain in Caries Prevention: A Systematic Review

Probiotic Streptococcus A12 Strain in Caries Prevention: A Systematic Review

Collagen Peptide in a Combinatorial Treatment with Lactobacillus rhamnosus Inhibits the Cariogenic Properties of Streptococcus mutans: An In Vitro Study

Concentration-Dependent Multi-Potentiality of L-Arginine: Antimicrobial Effect, Hydroxyapatite Stability, and MMPs Inhibition

Contact Info

Product

Resources

About