Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices

Pabst, Andreas; Wagner, Willi L.; Kašaj, Adrian; Gebhardt, Sascha; Ackermann, Maximilian; Astolfo, Alberto; Marone, Federica; Haberthür, David; Enzmann, Frieder; Konerding, Moritz A.

doi:10.1007/s00784-014-1312-4

Cited by 13 publications

(5 citation statements)

References 7 publications

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“…At the same time, the collagen matrix Mucoderm had a more expressed effect on gingival tissue regeneration than Mucograft, evidenced by histological examination showing an increase in cell proliferation and angiogenesis and further confirmed by immunohistochemical staining, that revealed abounded expression of IGF1 and VEGF. At the same time, the differences between the physical properties of Mucoderm and Mucograft collagen matrices were previously noted [ 15 , 16 ]. However, differences in the biochemical composition and cytotoxic properties of these materials were not previously identified.…”

Section: Discussionmentioning

confidence: 83%

See 1 more Smart Citation

Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits

Коган

Brailovskaya

et al. 2021

Dentistry Journal

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Soft gingival tissue deficiency remains a severe problem leading to postoperative recession, peri-implantitis, and bone resorption. The use of collagen matrices does not always lead to complete rebuilding of the gingiva volume. The application of mesenchymal stromal cells (MSCs) simultaneously with collagen materials represents a promising approach for the restoration of soft gingival tissues. However, short-term effects of MSCs-enriched collagen grafts after gingival augmentation have not yet been studied properly. Mucograft and Mucoderm matrices were implanted in rabbits (n = 12) simultaneously with the intraoperative injection of rabbit bone marrow-derived mesenchymal stromal cells (BM-MSCs) or without cells. Collagen matrices were implanted under the flap or by the surface technique without intentional primary closure. The samples were harvested seven days after implantation, histological staining with hematoxylin and eosin, and immunohistochemical staining for VEGF, IGF1, and TGF were performed. The use of Mucoderm led to better augmentation outcomes on day 7 compared with Mucograft (p < 0.0001). Gingival augmentation in combination with the local administration of BM-MSCs led to better regeneration of the soft gingival tissues independently of the type of implanted collagen matrices (p < 0.0001). Furthermore, injection of BM-MSCs significantly enhanced gingival vascularization and epithelization with a clear positive correlation between vascular growth and epithelial response. Administration of BM-MSCs in combination with various collagen materials may potentially improve gingiva regeneration.

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Section: Discussionmentioning

confidence: 83%

“…Mucograft (Geistlich Pharma AG, Wolhusen, Switzerland) is a 3D collagen matrix consisting of highly purified porcine collagen type I and III without cross-links, containing two layers of dense collagen and a spongy scaffold [ 15 , 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits

Коган

Brailovskaya

et al. 2021

Dentistry Journal

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“…20 XDM is a porcine dermis-derived acellular collagen matrix consisting of three-dimensional type I/III collagen matrix and elastin. 21,22 Compared with AADM, XDM has greater availability and can be harvested in bigger quantities. 21 According to Lin et al, XDM provides a favorable environment for promoting migration, adhesion, and proliferation of periodontal ligament and oral fibroblasts cells.…”

Section: Introductionmentioning

confidence: 99%

Allogenic Acellular Dermal Matrix and Xenogeneic Dermal Matrix as Connective Tissue Graft Substitutes for Long-Term Stability Gingival Recession Therapy: A Systematic Review and Meta-Analysis

Halim,

Sulijaya

2023

Eur J Dent

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Connective tissue graft (CTG) serves as a gold standard for gingival recession therapy. Yet the availability of CTG is limited, and it increases patient morbidity. Allogenic acellular dermal matrix (AADM) and xenogeneic dermal matrix (XDM) have been proven to be effective substitutes of CTG although the long-term stability is unclear. The aim of this study was to analyze the long-term stability outcome of gingival recession therapy using AADM and XDM compared to CTG. This study follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Data were extracted independently from several online databases (PubMed, Scopus, and Embase). Five of 233 publications were included for final qualitative analysis and meta-analysis focusing on the mean difference of clinical parameters such as recession depth (RD), recession width (RW), probing depth (PD), clinical attachment loss (CAL), tissue thickness (TT), keratinized tissue width (KTW), and mean root coverage (MRC). Meta-analyses of RD, RW, CAL, TT, KTW, and MRC display an overall mean of 0.2 mm (95% confidence interval [CI]: –0.45 to –0.05), 0.29 mm (95% CI: –0.65 to 0.08), 0.2 mm (95% CI: –0.69 to 0.29), 0.25 mm (95% CI: –0.53 to 0.03), 0.26 mm (95% CI: –0.5 to 0.02), and 9.19% (95% CI: –13.95 to –4.43]), respectively, favoring the CTG. PD was the only parameter that favored the AADM or XDM with an overall mean of 0.03 mm (95% CI: –0.05 to 0.11). In all, if the long-term stability is the goal, the CTG is considered superior for gingival recession therapy. However, if it is contraindicated, the AADM and XDM might be considered as alternatives.

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“…Full coverage ensures the growth of blood vessels and cells starting from the overlapped flap, and thus, the rapid incorporation of the graft 11 . Early exposure may lead to rapid absorption and contamination of the collagen matrix, causing graft failure of soft tissue 12 .…”

Section: ………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%

Use of Mucoderm® Collagen Matrix in Peri-Implant Aesthetic Defect Correction.

Prando¹

2018

IJAR

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Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices

Abstract: SRXTM may help to understand the clinical characteristics of 3D-CMs in more detail.

Cited by 13 publications

References 7 publications

Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits

Mesenchymal Stromal Cells Enhance Vascularization and Epithelialization within 7 Days after Gingival Augmentation with Collagen Matrices in Rabbits

Allogenic Acellular Dermal Matrix and Xenogeneic Dermal Matrix as Connective Tissue Graft Substitutes for Long-Term Stability Gingival Recession Therapy: A Systematic Review and Meta-Analysis

Use of Mucoderm® Collagen Matrix in Peri-Implant Aesthetic Defect Correction.

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