Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures

Chwiej, Joanna; Dulinska, J.; Janeczko, Krzysztof; Dumas, Paul; Eichert, Diane; Dudała, Joanna; Setkowicz, Zuzanna

doi:10.1016/j.jchemneu.2010.03.008

Cited by 42 publications

(60 citation statements)

References 41 publications

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“…IR imaging of a sector 3 in the Ammon's horn (CA3) and dentate gyrus (dg) of the hippocampus revealed the presence of the amide I band at 1657 and 1631 cm −1 in the control and diseased tissues, respectively. this shift is accompanied by a significant decrease in the absorbance at 1657 cm −1 [134]. Protein conformation changes towards β-sheet structures appear along with an increase in content of saturated phospholipids in the pathological hippocampus, especially in pyramidal cells of CA3 as well as in multiform and molecular layers of the dg.…”

Section: Brain Tissuementioning

confidence: 91%

“…this type of plaque appears in both normal and Ad aged brain, indicating that separate processes are responsible for its formation, whereas the core-dense plaques are typical for the Ad disorder [132]. Interestingly, an animal model of pilocarpine-evoked epilepsy shows spectral features similar to Alzheimer's disease [134,135]. IR imaging of a sector 3 in the Ammon's horn (CA3) and dentate gyrus (dg) of the hippocampus revealed the presence of the amide I band at 1657 and 1631 cm −1 in the control and diseased tissues, respectively.…”

Section: Brain Tissuementioning

confidence: 99%

“…the appearance of protein and phospholipid structural changes together may also be indicative of a process responsible for seizure activity [135]. In addition, creatine inclusions accompanied the β-sheet protein deposits [134]. Few inclusions were observed in the healthy brain sections, whereas the number of creatine deposits increased to 100 in the area of the dg and the multiform hippocampal at the acute phase of status epilepticus.…”

Section: Brain Tissuementioning

confidence: 99%

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FTIR Imaging of Tissues: Techniques and Methods of Analysis

Malek

Wood

Bambery

2013

Challenges and Advances in Computational Chemistry and Physics

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In this chapter, we describe biomedical applications of infrared microscopic imaging applied to human tissue sections. the central focus is human diseases including cervical cancer, neurodegenerative pathologies, and dysfunctions of cardiac and liver tissues. In addition, we briefly describe the fundamentals of FtIR imaging instrumentation along with spectral pre-processing and hyperspectral image reconstruction. the chapter concludes with a summary of what is required to take FtIR imaging technology into the clinical environment.

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Section: Brain Tissuementioning

confidence: 91%

Section: Brain Tissuementioning

confidence: 99%

Section: Brain Tissuementioning

confidence: 99%

See 1 more Smart Citation

FTIR Imaging of Tissues: Techniques and Methods of Analysis

Malek

Wood

Bambery

2013

Challenges and Advances in Computational Chemistry and Physics

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“…The specimens were cut into sections 10 µm thick in a cryo-microtome, mounted on silver-coated sample supports (Low-e MirrIR, Kevley Technologies) and freeze-dried at -80°C. It has been demonstrated that dried tissue mounted onto this sample support is durable, and the applied method is used as routine procedure of tissue sample preparation for infrared microspectroscopy [12].…”

Section: Sample Preparationmentioning

confidence: 99%

Investigation of biochemical composition of adrenal gland tumors by means of FTIR

Dudała¹,

Białas²,

Szczerbowska-Boruchowska³

et al. 2016

pjp

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The application of Fourier transform infrared (FTIR) microspectroscopy for the analysis of biomolecular composition of adrenal gland tumors is described. Samples were taken intraoperatively from three types of adrenal lesions: adrenal adenoma (ACA), adrenal cortical hyperplasia (ACH), both derived from adrenal cortical cells, and pheochromocytoma (Ph) derived from chromaffin cells of the adrenal medulla. The specimens were cryo-sectioned and freeze-dried. Since the investigated lesions originated from different cell types, it was predictable that they might differ in biomolecular composition. The experimental results were used to determine which absorption bands differentiate the analyzed samples the most. The main difference was observed in the lipid functional groups. The experimental results indicated that the level of lipids was higher in both the adenoma and the hyperplasia samples compared to pheochromocytomas. In contrast, the level of proteins was higher in the pheochromocytomas. Furthermore, differences within the range of nucleic acids and carbohydrates were observed in the studied adrenal gland tumor types.

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“…FTIRM has the outstanding advantages with synchrotron radiation (SR) infrared source due to its 100-1000 times higher brightness than a conventional thermal infrared source in the mid-IR regime, which can obtain high spatial resolution by achieving diffraction-limited spatial resolution [11][12][13]. FTIRM directly reflects characteristic changes in molecular composition and structure with disease states in detail by providing the information on vibrational motions of functional molecular groups such as lipids [14][15][16] and proteins [17,18]. Numerous studies demonstrated the applications of FTIRM in the biomedical area, such as cell death [19], Alzheimer's disease [20,21], osteoporosis [22], scrapie [23,24], the heart disease [25], and others [26].…”

Section: Introductionmentioning

confidence: 99%

Synchrotron FTIR Microspectroscopy Study of the Striatum in 6-Hydroxydopamine Rat Model of Parkinson's Disease

Zhu

Xiao

et al. 2012

Spectroscopy: An International Journal

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In the present study, synchrotron-based Fourier transform-infrared (FTIR) microspectroscopy is used to analyze the biochemical composition of the striatal neurons in normal and Parkinson's disease (PD) rat brain tissues. The rat model of Parkinson's disease is established by destroying the nigrostriatal pathway with 6-hydroxydopamine (6-OHDA). The detailed spectral analyses show the significant changes of cellular compositions such as lipids, and proteins in the striatal neurons of 6-OHDA-lesioned PD rats with respect to control neurons. As a result, the intensities of spectral absorption assigned to lipid of the striatal neurons in PD rats are higher than in control animals. Furthermore, the unsaturation levels of phospholipids decrease in PD neurons with respect to control neurons, indicating a high level of lipid peroxidation. The analysis of protein secondary structure shows the significantly higher ratio ofβ-sheet in PD neurons compared to that of control neurons, suggesting that the abnormal protein structure occurs before their morphological appearances in the striatal neurons. These findings suggest that the biochemical changes in neurons could be involved in the pathogenesis of Parkinson's disease.

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Synchrotron FTIR micro-spectroscopy study of the rat hippocampal formation after pilocarpine-evoked seizures

Cited by 42 publications

References 41 publications

FTIR Imaging of Tissues: Techniques and Methods of Analysis

FTIR Imaging of Tissues: Techniques and Methods of Analysis

Investigation of biochemical composition of adrenal gland tumors by means of FTIR

Synchrotron FTIR Microspectroscopy Study of the Striatum in 6-Hydroxydopamine Rat Model of Parkinson's Disease

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