Synchrotron-Infrared Microscopy Analysis of Amyloid Fibrils Irradiated by Mid-Infrared Free-Electron Laser

Kawasaki, Takayasu; Yaji, Toyonari; Imai, Takeshi; Ohta, Toshiaki; Tsukiyama, Koichi

doi:10.4236/ajac.2014.56047

Cited by 14 publications

(31 citation statements)

References 28 publications

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“…20 Experimentally, amyloid fibrils can be detected and removed with chemicals such as guanidine hydrochloride or dimethyl sulfoxide. 21 Since these chemicals are highly toxic and harmful, alternative methods using ultrasound 12,22,23 and infrared laser excitation [24][25][26][27][28] have been developed. It has been shown that amyloid fibrils are fragmented due to the ultrasound-induced bubble inertial cavitation.…”

Section: Introductionmentioning

confidence: 99%

Nonequilibrium all-atom molecular dynamics simulation of the bubble cavitation and application to dissociate amyloid fibrils

Man

Derreumaux

Nguyen

2016

The Journal of Chemical Physics

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The cavitation of gas bubbles in liquids has been applied to different disciplines in life and natural sciences, and in technologies. To obtain an appropriate theoretical description of effects induced by the bubble cavitation, we develop an all-atom nonequilibrium molecular-dynamics simulation method to simulate bubbles undergoing harmonic oscillation in size. This allows us to understand the mechanism of the bubble cavitation-induced liquid shear stress on surrounding objects. The method is then employed to simulate an Aβ fibril model in the presence of bubbles, and the results show that the bubble expansion and contraction exert water pressure on the fibril. This yields to the deceleration and acceleration of the fibril kinetic energy, facilitating the conformational transition between local free energy minima, and leading to the dissociation of the fibril. Our work, which is a proof-of-concept, may open a new, efficient way to dissociate amyloid fibrils using the bubble cavitation technique, and new venues to investigate the complex phenomena associated with amyloidogenesis.

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Section: Introductionmentioning

confidence: 99%

Nonequilibrium all-atom molecular dynamics simulation of the bubble cavitation and application to dissociate amyloid fibrils

Man

Derreumaux

Nguyen

2016

The Journal of Chemical Physics

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“…In our previous studies, the light of wavelength at 6.0-6.2 μm was strongly absorbed in amyloid fibril structures [5][6][7][8]. This wavelength range corresponds to the amide I band (C = O stretch vibration mode).…”

Section: Resultsmentioning

confidence: 96%

“…The structure and function of the FEL was described in detail in previous studies [5][6][7][8][9]. The energy value used for the current experiment was in the range of 8.0-9.0 mJ, and the full-width at half maximum of the FEL waveform is 0.1-0.2 μm.…”

Section: Mid-infrared Felmentioning

confidence: 99%

“…We recently discovered that a high-peak powered FEL, which has oscillation wavelengths at amide I band (C = O stretch vibration mode, 6.0-6.2 μm), can dissociate the aggregate forms of various proteins such as insulin, lysozyme, calcitonin fragment, and keratin to their monomer structures [5][6][7][8][9]. The FEL is operated as a picosecond pulsed laser with perfect linear polarization, in which one micro-pulse of 2 ps width is separated at 350 picosecond interval and one macro-pulse of 2-μs duration is structured by about 6000 of micro-pulses.…”

Section: Introductionmentioning

confidence: 99%

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Picosecond pulsed infrared laser tuned to amide I band dissociates polyglutamine fibrils in cells

et al. 2016

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Amyloid fibrils are causal substances for serious neurodegenerative disorders and amyloidosis. Among them, polyglutamine fibrils seen in multiple polyglutamine diseases are toxic to neurons. Although much efforts have been made to explore the treatments of polyglutamine diseases, there are no effective drugs to block progression of the diseases. We recently found that a free electron laser (FEL), which has an oscillation wavelength at the amide I band (C = O stretch vibration mode) and picosecond pulse width, was effective for conversion of the fibril forms of insulin, lysozyme, and calcitonin peptide into their monomer forms. However, it is not known if that is also the case in polyglutamine fibrils in cells. We found in this study that the fibril-specific β-sheet conformation of polyglutamine peptide was converted into nonfibril form, as evidenced by the infrared microscopy and scanning-electron microscopy after the irradiation tuned to 6.08 μm. Furthermore, irradiation at this wavelength also changed polyglutamine fibrils to their nonfibril state in cultured cells, as shown by infrared mapping image of protein secondary structure. Notably, infrared thermography analysis showed that temperature increase of the cells during the irradiation was within 1 K, excluding thermal damage of cells. These results indicate that the picosecond pulsed infrared laser can safely reduce amyloid fibril structure to the nonfibril form even in cells.

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“…The initial idea was brought by an observation that multiple protein aggregates can absorb most photon energy at the amide I band. Indeed, the structures of aggregated proteins was changed by a FEL tuned to the amide bands [13,[27][28][29][30].…”

Section: Polyglutamine Diseasementioning

confidence: 99%

Infrared light as a potential therapeutic approach for neurodegeneration

Nakamura¹,

Kawasaki²

2017

Biomed Res Clin Prac

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Neurodegenerative disorders such as Alzheimer's disease and Huntington's disease are devastating diseases that lead to cognitive and/or behavioral impairments. The misfolded causative molecules of these disorders form toxic aggregates that eventually induce synaptic dysfunction and neuronal cell death. To date, several drugs have been demonstrated to be beneficial for a substantial number of patients by improving their behavioral signs and symptoms and cognition. However, these drugs essentially target symptomatic treatments and leave the toxic aggregates intact. As the result, the effects of these drugs last only for a short period of time after drug administration. Recently, laser irradiations with near-and mid-infrared light have been tested to cultured cells and model animals of neurodegenerative disorders. The beneficial effects of the laser irradiation include restoration of intracellular organelle and dissociation of toxic aggregates. Laser irradiation might have a potential to be applied for treatments of multiple neurodegenerative disorders provided that appropriate dosages and their safety are thoroughly established.

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Synchrotron-Infrared Microscopy Analysis of Amyloid Fibrils Irradiated by Mid-Infrared Free-Electron Laser

Cited by 14 publications

References 28 publications

Nonequilibrium all-atom molecular dynamics simulation of the bubble cavitation and application to dissociate amyloid fibrils

Nonequilibrium all-atom molecular dynamics simulation of the bubble cavitation and application to dissociate amyloid fibrils

Picosecond pulsed infrared laser tuned to amide I band dissociates polyglutamine fibrils in cells

Infrared light as a potential therapeutic approach for neurodegeneration

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