Synchrotron Radiation Microtomography Allows the Analysis of Three-Dimensional Microarchitecture and Degree of Mineralization of Human Iliac Crest Biopsy Specimens: Effects of Etidronate Treatment

Nuzzo, S.; Lafage-Proust, Marie-Hélène; Martı́n-Badosa, Estela; Boivin, Georges; Thomas, Thierry; Alexandre, Christian; Peyrin, Françoise

doi:10.1359/jbmr.2002.17.8.1372

Cited by 157 publications

(87 citation statements)

References 52 publications

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“…The meshes, being built of connected wires, had SMI values of 2.48-3.09 and were thus identified as mostly rod-like structures. From a practical point of view, the small size of the presented calibration phantom (13 mm diameter, 23 mm height) allows its use with commonly used microCT systems, which can be of commercial type, custom made (Rossi et al, 2002) or based on synchrotron sources (Nuzzo et al, 2002). Moreover, considering the thin structures contained in it, embedding in PMMA makes the phantom easy to handle and preserves it over time.…”

Section: Discussionmentioning

confidence: 99%

A physical phantom for the calibration of three‐dimensional X‐ray microtomography examination

Perilli

Baruffaldi

Bisi

et al. 2006

Journal of Microscopy

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SummaryX-ray microtomography is rapidly gaining importance as a non-destructive investigation technique, especially in the three-dimensional examination of trabecular bone. Appropriate quantitative three-dimensional parameters describing the investigated structure were introduced, such as the modelindependent thickness and the structure model index. The first parameter calculates a volume-based thickness of the structure in three dimensions independent of an assumed structure type. The second parameter estimates the characteristic form of which the structure is composed, i.e. whether it is more plate-like, rod-like or even sphere-like. These parameters are now experiencing a great diffusion and are rapidly growing in importance. To measure the accuracy of these threedimensional parameters, a physical three-dimensional phantom containing different known geometries and thicknesses, resembling those of the examined structures, is needed. Unfortunately, such particular phantoms are not commonly available and neither does a consolidated standard exist. This work describes the realization of a calibration phantom for three-dimensional X-ray microtomography examination and reports an application example using an X-ray microtomography system. The calibration phantom (external size 13 mm diameter, 23 mm height) was based on various aluminium inserts embedded in a cylinder of polymethylmethacrylate. The inserts had known geometries (wires, foils, meshes and spheres) and thicknesses (ranging from 20 µ m to 1 mm). The phantom was successfully applied to an X-ray microtomography device, providing imaging of the inserted structures and calculation of three-dimensional parameters such as the modelindependent thickness and the structure model index. With the indications given in the present work it is possible to design a similar phantom in a histology laboratory and to adapt it to the requested applications.

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Section: Discussionmentioning

confidence: 99%

A physical phantom for the calibration of three‐dimensional X‐ray microtomography examination

Perilli

Baruffaldi

Bisi

et al. 2006

Journal of Microscopy

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“…Parameters similar to those used in histomorphometry were computed using a 3D version of the mean intercept length (MIL) method [25,44]. For random directions in 3D space, the number of intercepts of a set of parallel test lines with the bone structure were computed and normalized by the total length of test lines.…”

Section: D Morphometric Analysismentioning

confidence: 99%

“…Despite these significant relationships, it has not been proven that the best estimation of cancellous bone mechanical properties from density measurements is provided by adding 2D structural parameters. Furthermore, the limitation of 2D images to estimate the 3D architecture of trabecular networks has been shown in several works [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Relationship between compressive properties of human os calcis cancellous bone and microarchitecture assessed from 2D and 3D synchrotron microtomography

Follet

Bruyère-Garnier

Peyrin

et al. 2005

Bone

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The aim of this study was to determine the contribution of 2D and 3D microarchitectural characteristics in the assessment of the mechanical strength of os calcis cancellous bone. A sample of cancellous bone was removed in a medio-lateral direction from the posterior body of calcaneus, taken at autopsy in 17 subjects aged 61-91 years. The sample was first used for the assessment of morphological parameters from 2D morphometry and 3D synchrotron microtomography (ACT) (spatial resolution = 10 Am). The 2D morphometry was obtained from three slices extracted from the 3D ACT images. Very good concordance was shown between 3D ACT slices and the corresponding physical histologic slices. In 2D, the standard histomorphometric parameters, fractal dimension, mean intercept length, and connectivity were computed. In 3D, histomorphometric parameters were computed using both the 3D mean intercept length method and model-independent techniques. The 3D fractal dimension and the 3D connectivity, assessed by Euler density, were also evaluated. The cubic samples were subjected to elastic compressive tests in three orthogonal directions (X, Y, Z) close to the main natural trabecular network directions. A test was performed until collapse of trabecular network in the main direction (Z). The mechanical properties were significantly correlated to most morphological parameters resulting from 2D and 3D analysis. In 2D, the correlation between the mechanical strength and bone volume/tissue volume was not significantly improved by adding structural parameters or connectivity parameter (nodes number/tissue volume). In 3D, one architectural parameter (the trabecular thickness, Tb.Th) permitted to improve the estimation of the compressive strength from the bone volume/tissue volume alone. However, this improvement was minor since the correlation with the BV/TV alone was high (r = 0.96). In conclusion, which is in agreement with the statistic's rules, we found, in this study, that the determination of the os calcis bone compressive strength using the 3D bone volume fraction cannot be improved by adding 3D architectural parameters.

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“…L'utilisation du rayonnement synchrotron nécessite une infrastructure lourde qui existe en France sur deux sites : le european synchrotron radiation facility (ESRF) à Grenoble et le synchrotron Soleil à Saint-Aubin. Cette méthode de recherche est utilisée pour valider les autres méthodes d'investigation, et a déjà été utilisée pour explorer l'os humain [17] et les conséquences des traitements thérapeutiques [18]. dits apparents et ne permettent d'analyser que la texture.…”

Section: Les Techniques D'imagerie De La Structure Osseuseunclassified

Méthodes d’évaluation de la microarchitecture de l’os trabéculaire humain

Chappard

2012

Med Sci (Paris)

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Synchrotron Radiation Microtomography Allows the Analysis of Three-Dimensional Microarchitecture and Degree of Mineralization of Human Iliac Crest Biopsy Specimens: Effects of Etidronate Treatment

Cited by 157 publications

References 52 publications

A physical phantom for the calibration of three‐dimensional X‐ray microtomography examination

A physical phantom for the calibration of three‐dimensional X‐ray microtomography examination

Relationship between compressive properties of human os calcis cancellous bone and microarchitecture assessed from 2D and 3D synchrotron microtomography

Méthodes d’évaluation de la microarchitecture de l’os trabéculaire humain

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