Synchrotron XRR study of soft nanofilms at the mica–water interface

Briscoe, Wuge H.; Speranza, Francesca; Li, Peixun; Konovalov, Oleg; Bouchenoire, Laurence; Stam, Jan van; Klein, Jacob; Jacobs, Robert M.; Thomas, Robert K.

doi:10.1039/c2sm07179g

Cited by 39 publications

(50 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The deviation in the results from 4 repeated measurements for each liposome samples was within 10%. [53][54][55][56] The SLBs were formed by incubating PEI-coated silicon/silica substrates in SUV-QDs dispersion for 3 h and then rinsing with PBS. XRR data was plotted as the reflectivity, ܴ, against momentum transfer vector along the perpendicular direction of the sample surface, ܳ ൌ 4ߨ ߣ ⁄ ‫݊݅ݏ‬ሺ2ߠ 2 ⁄ ሻ where ߠ is the angle of incidence.…”

Section: Quartz Crystal Microbalancementioning

confidence: 99%

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

et al. 2018

Self Cite

View full text Add to dashboard Cite

Understanding interactions between functional nanoparticles and lipid bilayers is important to many emerging biomedical and bioanalytical applications. In this paper, we report incorporation of hydrophobic cadmium sulphide quantum dots (CdS QDs) into mixed 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) liposomes, and into their supported bilayers (SLBs). The QDs were found embedded in the hydrophobic regions of the liposomes and the supported bilayers, which retained the QD fluorescent properties. In particular, we studied the effect of the QD size (2.7-5.4 nm in diameter) on the formation kinetics and structure of the supported POPC/POPE bilayers, monitored in situ using quartz crystal microbalance with dissipation monitoring (QCM-D), as the liposomes ruptured onto the substrate. The morphology of the obtained QD-lipid hybrid bilayers was studied using atomic force microscopy (AFM), and their structure by synchrotron X-ray reflectivity (XRR). It was shown that the incorporation of hydrophobic QDs promoted bilayer formation on the PEI cushion, evident from the rupture and fusion of the QD-endowed liposomes at a lower surface coverage compared to the liposomes without QDs. Furthermore, the degree of disruption in the supported bilayer structure caused by the QDs was found to be correlated with the QD size. Our results provide mechanistic insights into the kinetics of the rupturing and formation process of QD-endowed supported lipid bilayers via liposome fusion on polymer cushions.

show abstract

Section: Quartz Crystal Microbalancementioning

confidence: 99%

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Synchrotron XRR measurements were performed at beamline BM28-XMaS, European Synchrotron Radiation Facility (ESRF), Grenoble, France. A custom-designed liquid cell shown schematically in Figure 1 [32,38,41] was used for XRR experiments, with graphene samples mounted on the sample stage. The cell was sealed by two polyester (Mylar®) windows, providing a total liquid capacity of 5 ml.…”

Section: Experimental Methodsmentioning

confidence: 99%

Graphene surface structure in aqueous media: Evidence for an air-bubble layer and ion adsorption

Zhou

Islas

Taylor

et al. 2019

Carbon

Self Cite

View full text Add to dashboard Cite

show abstract

“…53 To address the above-mentioned factors such as surface charge and hydrophilicity of PEM cushions that affect formation of supported lipid bilayers, in this work we have focused on characterising wetting, surface free energy and zeta potential of a number of PEMs. Furthermore, the structure of PEMs was also characterized by X-ray reflectometry (XRR), which has been used to characterise soft nanofilms [54][55] and previously used to characterise PEMs. 18 XRR can yield useful structural details on the thickness, roughness and electron density, which could be correlated with the physical density of PEMs.…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 99%

“…The XRR measurements [54][55]57 were performed at beamline BM28 at the European Synchrotron Radiation Facility (ESRF), Grenoble, France. The BM28 monochromator was tuned to select an X-ray beam energy of 14keV with a corresponding wavelength λ=0.886Å.…”

Section: Synchrotron X-ray Reflectometry (Xrr) Measurementsmentioning

confidence: 99%

Interfacial and structural characteristics of polyelectrolyte multilayers used as cushions for supported lipid bilayers

et al. 2017

View full text Add to dashboard Cite

The surface properties of polyelectrolyte multilayers (PEMs) obtained via sequential adsorption of oppositely charged polyions from their solutions and used as cushions for supported lipid bilayers were investigated. Five types of polyelectrolytes were used: cationic polyethyleneimine (PEI), poly(diallyldimethylammonium)chloride (PDADMAC), and poly-l-lysine hydrobromide (PLL); and anionic polysodium 4-styrenesulfonate (PSS) and poly-l-glutamic acid sodium (PGA). The wettability and surface free energy of the PEMs were determined by contact angle measurements using sessile drop analysis. Electrokinetic characterisation of the studied films was performed by streaming potential measurements of selected multilayers and the structure of the polyelectrolyte multilayer was characterized by synchrotron X-ray reflectometry. The examined physicochemical properties of the PEMs were correlated with the kinetics of the formation of supported lipid bilayers atop the PEM cushion.

show abstract

Synchrotron XRR study of soft nanofilms at the mica–water interface

Cited by 39 publications

References 91 publications

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

Supported lipid bilayers with encapsulated quantum dots (QDs) via liposome fusion: effect of QD size on bilayer formation and structure

Graphene surface structure in aqueous media: Evidence for an air-bubble layer and ion adsorption

Interfacial and structural characteristics of polyelectrolyte multilayers used as cushions for supported lipid bilayers

Contact Info

Product

Resources

About