Synergic effect between TRAIL gene and curcumin in magnetic chitosan nanoparticles on cancer cells apoptosis enhanced by laser photoactivation

Alvizo-Báez, Cynthia Aracely; Peña-Torres, Arnulfo Aramis; Terrazas-Armendariz, Luis D; Luna-Cruz, Itza Eloisa; Uscanga‐Palomeque, Ashanti Concepción; Sampayo-Reyes, Adriana; Taméz-Guerra, Reyes; Rodríguez‐Padilla, Cristina; Alcocer‐González, Juan Manuel

doi:10.1007/s11051-022-05544-x

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…To prove the efficiency of our vector, it was encapsulated in chitosan magnetic nanoparticles, called Magnetogene, with a final size of 60 nm by SEM and 218 nm by DLS ( Figure 2 ). DLS requires the sample to be dispersed in solution, while for SEM, a dry flat surface sample is used, and the difference in the sample can influence the morphology of the Magnetogene due to the flexible nature of the chitosan polymer [ 20 , 28 ]. Magnetogene has a Zeta potential of +15.9 mV which is a critical parameter for cellular interaction; interactions with pDNA-NP complexes indicate that the complexes were positively charged.…”

Section: Discussionmentioning

confidence: 99%

“…Among these polymers, chitosan is an attractive drug or gene delivery system [ 17 , 18 ]. Magnetic drug delivery systems work via the delivery of magnetic nanoparticles loaded with the drug to the tumor site under the influence of an external magnetic field [ 19 , 20 ]. In vivo magnetic fields are focused on the required site to promote transfection and to deliver the therapeutic gene to a specific organ or site within the body.…”

Section: Introductionmentioning

confidence: 99%

“…In vivo magnetic fields are focused on the required site to promote transfection and to deliver the therapeutic gene to a specific organ or site within the body. Many different tissues have been studied as potential magnetic drug delivery targets [ 20 , 21 ]. The main objective of this study was to construct a vector for selective gene expression in the hypoxic microenvironments of mouse solid tumors through the development of the Magnetogene nanoparticle vector.…”

Section: Introductionmentioning

confidence: 99%

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Systemic Delivery of Magnetogene Nanoparticle Vector for Gene Expression in Hypoxic Tumors

Terrazas-Armendáriz,

Alvizo-Báez,

Luna-Cruz

et al. 2023

Pharmaceutics

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Cancer is a disease that causes millions of deaths per year worldwide because conventional treatments have disadvantages such as unspecific tumor selectivity and unwanted toxicity. Most human solid tumors present hypoxic microenvironments and this promotes multidrug resistance. In this study, we present “Magnetogene nanoparticle vector” which takes advantage of the hypoxic microenvironment of solid tumors to increase selective gene expression in tumor cells and reduce unwanted toxicity in healthy cells; this vector was guided by a magnet to the tumor tissue. Magnetic nanoparticles (MNPs), chitosan (CS), and the pHRE-Luc plasmid with a hypoxia-inducible promoter were used to synthesize the vector called “Magnetogene nanoparticles” by ionic gelation. The hypoxic functionality of Magnetogene vector nanoparticles was confirmed in the B16F10 cell line by measuring the expression of the luciferase reporter gene under hypoxic and normoxic conditions. Also, the efficiency of the Magnetogene vector was confirmed in vivo. Magnetogene was administered by intravenous injection (IV) in the tail vein and directed through an external magnetic field at the site of tumor growth in C57Bl/6 mice. A Magnetogene vector with a size of 50 to 70 nm was directed and retained at the tumor area and gene expression was higher at the tumor site than in the others tissues, confirming the selectivity of this vector towards hypoxic tumor areas. This nanosystem, that we called the “Magnetogene vector” for systemic delivery and specific gene expression in hypoxic tumors controlled by an external magnetic designed to target hypoxic regions of tumors, can be used for cancer-specific gene therapies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Systemic Delivery of Magnetogene Nanoparticle Vector for Gene Expression in Hypoxic Tumors

Terrazas-Armendáriz,

Alvizo-Báez,

Luna-Cruz

et al. 2023

Pharmaceutics

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“…This was done either alone or in combination with the aim of overexpressing the receptors required for apoptosis induction. Results revealed a significant increase in cellular death within 48 h when using a combination of TRAIL gene-based and drug-based systems compared to each nanocomplex individually, indicating a synergistic effect [127].…”

Section: Genesmentioning

confidence: 97%

Metal and Metal Oxides Nanoparticles and Nanosystems in Anticancer and Antiviral Theragnostic Agents

et al. 2023

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The development of antiviral treatment and anticancer theragnostic agents in recent decades has been associated with nanotechnologies, and primarily with inorganic nanoparticles (INPs) of metal and metal oxides. The large specific surface area and its high activity make it easy to functionalize INPs with various coatings (to increase their stability and reduce toxicity), specific agents (allowing retention of INPs in the affected organ or tissue), and drug molecules (for antitumor and antiviral therapy). The ability of magnetic nanoparticles (MNPs) of iron oxides and ferrites to enhance proton relaxation in specific tissues and serve as magnetic resonance imaging contrast agents is one of the most promising applications of nanomedicine. Activation of MNPs during hyperthermia by an external alternating magnetic field is a promising method for targeted cancer therapy. As therapeutic tools, INPs are promising carriers for targeted delivery of pharmaceuticals (either anticancer or antiviral) via magnetic drug targeting (in case of MNPs), passive or active (by attaching high affinity ligands) targeting. The plasmonic properties of Au nanoparticles (NPs) and their application for plasmonic photothermal and photodynamic therapies have been extensively explored recently in tumor treatment. The Ag NPs alone and in combination with antiviral medicines reveal new possibilities in antiviral therapy. The prospects and possibilities of INPs in relation to magnetic hyperthermia, plasmonic photothermal and photodynamic therapies, magnetic resonance imaging, targeted delivery in the framework of antitumor theragnostic and antiviral therapy are presented in this review.

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“…Curcumin (Cur) and chitosan (Chi) encapsulation within MNP was performed according to previous reports (22,23). Water-soluble Chi (20 kDa) was purchased from Coyote Foods Biopolymer and Biotechnology (Saltillo, Coahuila, Mexico) and resuspended in MilliQ water at a concentration of 2 mg/mL.…”

Section: Cur-chi-mnp Synthesismentioning

confidence: 99%

Biofilm Eradication and Inhibition of Methicillin-Resistant <i>Staphylococcus</i> Clinical Isolates by Curcumin-Chitosan Magnetic Nanoparticles

Salazar-Sesatty,

Montoya-Hinojosa,

Villarreal-Salazar

et al. 2024

Jpn J Infect Dis

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Biofilm-producing methicillin-resistant Staphylococcus aureus (MRSA) and coagulasenegative Staphylococci (MR-CoNS) are a clinical challenge for the treatment of healthcareassociated infections. As alternative antimicrobial options are needed, we aimed to determine the effect of curcumin-chitosan magnetic nanoparticles on the biofilm of staphylococcal clinical isolates. MRSA and CoNS clinical isolates were identified by MALDI-TOF mass spectrometry. Antimicrobial susceptibility testing was performed by broth microdilution. Nanoparticles were synthesized by co-precipitation of magnetic nanoparticles (MNP) and encapsulation by ionotropic gelation of curcumin (Cur) and chitosan (Chi). Biofilm inhibition and eradication by nanoparticles with and without the addition of oxacillin was assessed on staphylococcal strains. Cur-Chi-MNP showed antimicrobial activity on planktonic cells of MRSA and MR-CoNS strains and inhibited biofilm of MRSA. The addition of OXA to Cur-Chi-MNP increased biofilm inhibition and eradication activity against all Staphylococci strains (p=0.0007); higher biofilm activity was observed in early biofilm stages. Cur-Chi-MNP showed antimicrobial and biofilm inhibition activity against S. aureus. The addition of OXA increased biofilm inhibition and eradication activity against all Staphylococci strains. A combination treatment of Cur-Chi-MNP and OXA could be potentially used to treat staphylococcal biofilm-associated infections in its early stages before the establishment of biofilm bacterial cells.

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Synergic effect between TRAIL gene and curcumin in magnetic chitosan nanoparticles on cancer cells apoptosis enhanced by laser photoactivation

Cited by 4 publications

References 31 publications

Systemic Delivery of Magnetogene Nanoparticle Vector for Gene Expression in Hypoxic Tumors

Systemic Delivery of Magnetogene Nanoparticle Vector for Gene Expression in Hypoxic Tumors

Metal and Metal Oxides Nanoparticles and Nanosystems in Anticancer and Antiviral Theragnostic Agents

Biofilm Eradication and Inhibition of Methicillin-Resistant <i>Staphylococcus</i> Clinical Isolates by Curcumin-Chitosan Magnetic Nanoparticles

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