Synergistic Antibacterial Effect from Silver Nanoparticles and Anticancer Activity Against Human Lung Cancer Cells

Abstract

Microbially synthesized silver nanoparticles (AgNPs) with high stability and bioactivity have recently shown considerable promise in biomedical research and application. In this study, AgNPs prepared by Penicillium aculeatum Su1 exhibited effective antibacterial action by inhibiting bacterial growth and destroying cellular structure. Meanwhile, their assessed increased in fold area (IFA) through the Kirby-Bauer disc diffusion method proved that, the AgNPs showed synergistic antibacterial effect on different bacteria when combined with antibiotics, especially for drug-resistant P. aeruginosa (4.58∼6.36-fold) and B. subtilis (4.2-fold). Moreover, the CCK-8 assay and flow cytometric analysis were used to evaluate the cytotoxic effects of AgNPs on normal cells (HBE) and lung cancer cells (HTB-182), which confirmed that they presented higher biocompatibility towards HBE cells when compared with silver ions, but high cytotoxicity in a dosedependent manner with an IC50 values of 35.00 μg/mL towards HTB-182 cells by raising intracellular reactive oxygen species (ROS) levels, hindering cell proliferation, and ultimately leading to cell cycle arrest and cell apoptosis. These results demonstrate that, the biosynthesized AgNPs could be a potential candidate for future therapies of infection caused by drug-resistant bacteria, as well as lung squamous cell carcinoma.

Synergistic Anticandidal Effectiveness of Greenly Synthesized Zinc Oxide Nanoparticles with Antifungal Agents against Nosocomial Candidal Pathogens

“…IFA = (B 2 − A 2 )/A 2 , whereas B and A are the inhibition zone diameters for antifungals + ZnO-NPs and antifungal agents, respectively [ 42 ]. Moreover, the fractional inhibitory concentration (FIC) index was estimated using the formula described in previous studies [ 43 , 44 ].…”

“…Finally, the plates were incubated at 35 • C for 24 h, and then a Vernier caliper was used to assess the diameters of inhibition zones after the incubation period. The increase in fold of inhibition area (IFA) was calculated in accordance with the subsequent equation: IFA = (B 2 − A 2 )/A 2 , whereas B and A are the inhibition zone diameters for antifungals + ZnO-NPs and antifungal agents, respectively [42]. Moreover, the fractional inhibitory concentration (FIC) index was estimated using the formula described in previous studies [43,44].…”

Green Biofabrication of Silver Nanoparticles of Potential Synergistic Activity with Antibacterial and Antifungal Agents against Some Nosocomial Pathogens

“…Finally, the plates were incubated at 35 • C overnight, and then inhibition zones were measured for determination of relative synergistic activity as demonstrated in the following equation: synergism % = B−A A × 100, whereas A and B are the inhibition zones for the standard antimicrobial agent and antimicrobial agent+ AgNPs, respectively. The increase in fold of inhibition area (IFA) was calculated according to the following equation [48]:…”