Synergistic antibacterial effects of<i>Buddleja albiflora</i>metabolites with antibiotics against<i>Listeria monocytogenes</i>

Zhang, X.; Wang, L.; Mu, Haibo; Wang, Dan; Yang, Yuanzheng

doi:10.1111/lam.13084

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…Kumatakenin exhibits anti-cancer activities by inducing cancer cell death and suppressing the polarization of tumor-associated macrophages (Woo et al, 2017). Kumatakenin promotes antibiotic-exerted effects used to treat pathogenic bacteria (Zhang et al, 2019). Kumatakenin also inhibits SARS-CoV-2 replication in Vero E6 and Calu-3 cells (Leal et al, 2021).…”

Section: Open Access Edited Bymentioning

confidence: 99%

Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis

et al. 2023

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Inhibition of epithelial ferroptosis in colonic tissues relieved clinical symptoms and improved endoscopic presentations in inflammatory bowel disease (IBD). Kumatakenin, the main ingredient of traditional Chinese medicinal cloves and Alpinia purpurata, is reported to possess therapeutic benefits. However, whether kumatakenin could inhibit ferroptosis and further alleviate colitis remains unclear. Here, we measured the effects of kumatakenin on ferroptosis of colonic epithelial cells from colitis mice. The colitis model was induced in mice by oral intake of 2.5% dextran sulfate sodium in drinking water. RNA sequencing was performed to investigate the mechanism underlying kumatakenin-mediated effects on colitis. The results showed that different doses of kumatakenin significantly alleviated symptoms and suppressed intestinal inflammation in the colitis mouse model. Kumatakenin supplementation decreased cellular iron levels and suppressed ferroptosis in epithelial cells from colitis mice. RNA sequencing, qPCR, and pharmacological inhibition assays showed that kumatakenin reduced cellular iron levels and suppressed ferroptosis in epithelial cells from colitis mice at least partially by upregulating expression of enolase (Eno-3). Furthermore, kumatakenin decreased iron levels in epithelial cells by modulating the Eno3-iron regulatory protein (IRP1) axis. Molecular docking results revealed that kumatakenin could bind Eno3 via hydrogen bonding with the amino acid residues Thr208, Val206, and Pro203. This work will provide a scientific basis for the clinical use of kumatakenin in the treatment of colitis.

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Section: Open Access Edited Bymentioning

confidence: 99%

Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis

et al. 2023

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“…For biofilm quantification, the bacterial biofilm formation protocol was applied according to our previous report. 48 A volume of 100 mL [10 8 colony-forming units (CFU) mL −1 ] S. aureus and Psa suspension prepared in LB medium was transferred to a 96-well microplate and incubated at 37 °C for 24 h to allow cells to adhere tightly and biofilms to form successfully. The suspension was removed with a pipette and the plate was washed carefully with 100 μL of 0.9% (w/v) NaCl.…”

Section: Biofilm Formation Assaymentioning

confidence: 99%

Pitsubcosides A–L, highly esterified eudesmane sesquiterpenoid glycosides with antibacterial activity from Pittosporum subulisepalum and their mechanism

Guan

Xia

Huang

et al. 2023

Pest Management Science

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BACKGROUNDPlants from the genus Pittosporum are traditionally used as antibacterial, antifungal and antiviral agents. A bioassay evaluation of the extract of Pittosporum subulisepalum revealed antibacterial activity. This study focused on the discovery of the antibacterial metabolism in P. subulisepalum, as well as the modes of action of its active components.RESULTSA chemical investigation of an ethyl acetate (EtOAc) extract of the aerial parts of P. subulisepalum led to the isolation of 12 previously undescribed eudesmane sesquiterpenoid glycoside esters (ESGEs), pitsubcosides A–L (1–12). Their structures were elucidated by extensive spectroscopic analysis, including one‐ and two‐dimensional NMR, high‐resolution electrospray ionization mass spectrometry, electronic circular dichroism spectra and single‐crystal X‐ray crystallography analysis or by comparing with authentic samples. The new ESGEs were characterized by their highly esterified glycoside moieties. Among them, compounds 1–3, 5 and 8 showed a moderate inhibitory effect against Staphylococcus aureus, methicillin‐resistant S. aureus (MRSA), Bacillus cereus, Bacillus subtilis, Pseudomonas syringae pv. actinidiae (Psa) and Erwinia carotovora with minimum inhibitory concentrations (MICs) ranging from 3.13 to 100 μm. Among them, compounds 3 and 5 showed remarkable antibacterial activity against S. aureus and Psa with MIC values of 6.25 and 3.13 μm, respectively. Live bacterial mass and the biofilms of S. aureus and Psa were quantified using methyl tetrazolium and crystal violet assays. Fluorescence microscopy and scanning electron microscopy experiments revealed an antibacterial mechanism of cell membrane architectural disruption.CONCLUSIONThe results suggest that ESGEs possess great potential for the development of antibacterial agents to control plant pathogens. © 2023 Society of Chemical Industry.

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“…, Psychotria serpens and Siparuna cristata ( Chen 2011 ; Xue et al, 2013 ). Some studies illustrated that jaranol had a potential role in inhibiting SARS-CoV-2 replication ( Leal et al, 2021 ) and exerting anti-cancer ( Woo et al, 2017 ), alpha-glucosidase inhibitory ( Dao et al, 2021 ), anti-bacterial ( Zhang et al, 2019 ), and anti-oxidant activities in vitro ( Quek et al 2021 ). At present, no studies evaluating the effect of jaranol in vivo were found from a PubMed search with the keywords jaranol or kumatakenin.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Acute and Sub-Acute Oral Toxicity of Jaranol in Kunming Mice

Liu

Zhang²,

Liu³

et al. 2022

Front. Pharmacol.

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Background: Jaranol has shown a wide range of pharmacological activities; however, no study has yet examined in vivo toxicity. The study aimed to investigate the oral acute and sub-acute toxicity of jaranol in mice.Methods: The acute toxicity was determined by a single oral dose of jaranol (2000 mg/kg). Therein animal behaviour and mortality rate were observed for 14 days. The jaranol (50, 100 and 200 mg/kg BW·d−1) was given by gavage for 28 days daily in the sub-acute study. The mouse body weight (BW), organ weight, food, water intake, biochemical, haematological parameters, and histopathology were studied in acute and sub-acute toxicity.Results: During the acute toxicity test, a single oral dose (2000 mg/kg) jaranol did not cause significant alteration in majority of the hematological indices. However, jaranol decreased the level of serum alanine aminotransferase and aspartate aminotransferase. Those results showed that the oral lethal dose 50 (LD50) of jaranol was higher than 2000 mg/kg BW, regardless of sex. In repeated daily oral doses (50, 100 and 200 mg/kg BW·d−1), no mortality was recorded in the various experimental groups. The jaranol reduced body weight gain (200 mg/kg BW·d−1), the relative spleen weight (all doses) and serum alanine aminotransferase activity (200 mg/kg BW·d−1). On the other hand, jaranol significantly elevated red blood cell count (100 and 200 mg/kg BW·d−1) and serum creatinine levels (200 mg/kg BW·d−1). Histological study revealed that spleen bleeding was identified in 200 mg/kg jaranol-treated mice.Conclusion: Jaranol was relatively safe in Kunming Mice when repetitively administered orally in small doses for a prolonged period of time. We recommend more chronic toxicity studies and clinical trials on jaranol to ensure that its use is free of potential toxicity to humans.

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Synergistic antibacterial effects ofBuddleja albiflorametabolites with antibiotics againstListeria monocytogenes

Cited by 8 publications

References 33 publications

Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis

Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis

Pitsubcosides A–L, highly esterified eudesmane sesquiterpenoid glycosides with antibacterial activity from Pittosporum subulisepalum and their mechanism

Evaluation of the Acute and Sub-Acute Oral Toxicity of Jaranol in Kunming Mice

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