Synergistic Anticancer Therapy by Ovalbumin Encapsulation‐Enabled Tandem Reactive Oxygen Species Generation

Jiang, Shuai; Xiao, Ming; Sun, Wen; Crespy, Daniel; Mailänder, Volker; Peng, Xiaojun; Fan, Jiangli; Landfester, Katharina

doi:10.1002/ange.202006649

Cited by 5 publications

(2 citation statements)

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“…In this study, we demonstrate that, by tailoring a densely packed “brush” conformation of PEG on ovalbumin nanocarriers (OVA-NCs), we can considerably reduce the uptake in cells. Ovalbumin nanocapsules were chosen because of their good biodegradability and low protein adsorption. , This low cell interaction is achieved by combining a brush conformation of PEG with low protein affinity of OVA-NCs, which minimizes the overall protein adsorption. OVA-NCs were covalently attached with PEG at varied lengths and densities, resulting in PEG chains with mushroom or brush conformation.…”

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Brush Conformation of Polyethylene Glycol Determines the Stealth Effect of Nanocarriers in the Low Protein Adsorption Regime

et al. 2021

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For nanocarriers with low protein affinity, we show that the interaction of nanocarriers with cells is mainly affected by the density, the molecular weight, and the conformation of polyethylene glycol (PEG) chains bound to the nanocarrier surface. We achieve a reduction of nonspecific uptake of ovalbumin nanocarriers by dendritic cells using densely packed PEG chains with a “brush” conformation instead of the collapsed “mushroom” conformation. We also control to a minor extent the dysopsonin adsorption by tailoring the conformation of attached PEG on the nanocarriers. The brush conformation of PEG leads to a stealth behavior of the nanocarriers with inhibited uptake by phagocytic cells, which is a prerequisite for successful in vivo translation of nanomedicine to achieve long blood circulation and targeted delivery. We can clearly correlate the brush conformation of PEG with inhibited phagocytic uptake of the nanocarriers. This study shows that, in addition to the surface’s chemistry, the conformation of polymers controls cellular interactions of the nanocarriers.

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Brush Conformation of Polyethylene Glycol Determines the Stealth Effect of Nanocarriers in the Low Protein Adsorption Regime

et al. 2021

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“…It was widely accepted that iron ions catalyzed H 2 O 2 to produce •OH via the Fenton reaction. [32][33][34][35][36] We chose 3,3',5,5'-tetramethylbenzidine (TMB) as •OH probe, which could be oxidized to oxTMB with an absorbance at nm. 32 The following control experiment was implemented to eliminate the possibility that MnS catalyzed the conversion of H 2 O 2 to 1 O 2 and affected the chromogenic reaction of TMB (Figure S10).…”

Section: Mri Ros Evaluation Gsh Depletion and Degradation Of Mnsmh Npsmentioning

confidence: 99%

Dual Reactive Oxygen Species Generator Independent of Light and Oxygen for Tumor Imaging and Catalytic Therapy

et al. 2022

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Currently, reactive oxygen species (ROS) generation primarily depends upon light and O 2 , which hampers its further biomedical application. Here, we reported that a manganese(III) salen-based complex (MnS) could continuously catalyze overexpressed hydrogen peroxide (H 2 O 2 ) in the tumor microenvironment to 1 O 2 , while the nanocarrier (MIL-100) as a Fenton reagent could convert H 2 O 2 to hydroxyl radicals (•OH) through the Fenton reaction, inducing noticeable intracellular DNA strand scission and lipid peroxidation to provoke tumor cell apoptosis without the involvement of light and O 2 .Moreover, MIL-100 depleted the antioxidant glutathione, further amplifying intracellular oxidative pressure, which in turn led to the self-degradation of MIL-100, suggesting the long-term biosafety of the nanoplatform. Owing to the excellent magnetic resonance imaging performance of MnS, the diagnosis and specific treatment of tumors were eventually achieved. This work provided a novel approach for the realization of effective tumor catalytic therapy independent of light and O 2 and a promising reference for the development of a wide range of catalytic therapeutic agents.

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Exquisite Vesicular Nanomedicine by Paclitaxel Mediated Co‐assembly with Camptothecin Prodrug

Zhou

Zhang

et al. 2021

Angewandte Chemie

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We report that the self‐assembly of drug amphiphiles, Evans blue conjugated camptothecin prodrug (EB‐CPT), can be modulated by another anticancer drug paclitaxel (PTX), resulting in ultrahigh quality of nanovesicles (NVs) with uniform shape and diameters of around 80 nm with the EB‐CPT:PTX weight ratio of 1:1, 1:2, and 1:3, denoted as ECX NVs. Significantly, the co‐assembly of EB‐CPT and PTX without adding other excipients has nearly 100 % drug loading efficiency (DLE) and ultrahigh drug loading content (DLC) of PTX alone of up to 72.3±1.7 wt % which, to our best knowledge, is among the highest level reported in literature. Moreover, the ECX NVs with the EB‐CPT:PTX weight ratio of 1:2 showed remarkable combination index of 0.59 at a level of 50 % efficacy against HCT116 cells in vitro and greatly improved tumor inhibition effect in vivo compared with two clinically approved CPT‐ and PTX‐based anticancer nanomedicines (Onivyde and Abraxane) individually and their combinations.

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Synergistic Anticancer Therapy by Ovalbumin Encapsulation‐Enabled Tandem Reactive Oxygen Species Generation

Cited by 5 publications

References 50 publications

Brush Conformation of Polyethylene Glycol Determines the Stealth Effect of Nanocarriers in the Low Protein Adsorption Regime

Brush Conformation of Polyethylene Glycol Determines the Stealth Effect of Nanocarriers in the Low Protein Adsorption Regime

Dual Reactive Oxygen Species Generator Independent of Light and Oxygen for Tumor Imaging and Catalytic Therapy

Exquisite Vesicular Nanomedicine by Paclitaxel Mediated Co‐assembly with Camptothecin Prodrug

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