Synergistic interaction between Astragali Radix and Rehmanniae Radix in a Chinese herbal formula to promote diabetic wound healing

Lau, Ken; Lai, Kwok-Kin; Liu, Cheuk‐Lun; Tam, Jacqueline Chor‐Wing; To, Ming-Ho; Kwok, Hin‐Fai; Lau, Clara Bik San; Ko, Chun‐Hay; Leung, Ping‐Chung; Fung, Kwok‐Pui; Poon, Simon K.; Lau, Clara Bik‐San

doi:10.1016/j.jep.2012.02.025

Cited by 116 publications

(73 citation statements)

References 38 publications

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“…Consistent to what we found in dose-dependent inhibition of RR aqueous crude extract (Lau et al, 2012), among the four successive partitions of RR crude extract (see Figs. 1 and 2), DCM partition (fraction P1) exhibited the most potent suppression of NO production (at 62.5 mg/ml, p o0.001).…”

Section: Resultssupporting

confidence: 91%

“…Recently, we also revealed that the RR crude extract could significantly and dose-dependently inhibit nitric oxide production (Lau et al, 2012). Indeed, RR crude extract have also been reported to inhibit inflammatory responses in vitro triggered by advanced glycation end products (Baek et al, 2012) and protected against cell damage via scavenging free radicals (Yu et al, 2006).…”

Section: Introductionmentioning

confidence: 94%

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Bioassay-guided isolation of anti-inflammatory components from the root of Rehmannia glutinosa and its underlying mechanism via inhibition of iNOS pathway

Liu

Cheng

et al. 2012

Journal of Ethnopharmacology

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 94%

Bioassay-guided isolation of anti-inflammatory components from the root of Rehmannia glutinosa and its underlying mechanism via inhibition of iNOS pathway

Liu

Cheng

et al. 2012

Journal of Ethnopharmacology

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“…Like mitogens, substances promoting angiogenesis would also promote healing process by supply of blood around the wound sites. Extracts of Aloe vera (54), Alternanthera brasiliana (58), mixtures of extracts from Astragali radix and Rehmanniae radix (519) (336), Nigella sativa (347) etc were claimed to be responsible for facilitated healing as evidenced by increased DNA production and total collagen enhancement at wound site with time. However, in all these cases individual specific chemical entities having the properties of enhancing the wound healing process have not been described.…”

Section: Active Plant Parts Studied For Wound Healingmentioning

confidence: 99%

Phyto-Extracts in Wound Healing

2013

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-Data generated through systematic investigation, carried out on the evaluation of phyto-extracts on wound healing research during the last 20 years have been compiled. About 450 plant species having wound healing properties have been identified. The present knowledge of the wound healing process comprise coagulation, inflammation, proliferation, formation and accumulation of fibrous tissues, collagen deposition, epithelialization, contraction of wound with formation of granulation tissues, remodeling and maturation. The constituents of the plant extracts modulate one or more of the above stages. It was the endeavor to identify the active constituents responsible for antimicrobial activity, free radical scavenging properties, stimulators of enhanced collagen production and/or angiogenesis promoters with identification of lead scaffold chemical structures. Multiple phytochemicals concentrated and blended in optimal concentrations, are expected to be available in future years to carry out multi-tasking efforts in wound healing as more knowledge about the properties of the key constituents are unveiled.

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“…The nitrite concentrations were calculated using sodium nitrite standard curve. The methodology was slightly modified from Lau et al [14].…”

Section: Standard Nitrite Assaymentioning

confidence: 99%

Anti-Inflammatory Activity of Bacteria Associated With Marine Sponge (Haliclona Amboinensis) via Reducting No Production and Inhibiting Cyclooxygenase-1, Cyclooxygenase-2, and Secretory Phospholipase A2 Activities

Andriani

Marlina

Mohamad

et al. 2017

Asian J Pharm Clin Res

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Objective: This study aimed to investigate the anti-inflammatory activity of methanol extract and fractions of bacteria associated with sponge (Haliclona amboinensis) and to evaluate their effect in reducing NO production and inhibiting cyclooxygenase-1 (COX-1), cyclooxgenase-2 (COX-2) and secretory phospholipase A2 (sPLA2) activity.Methods: All bacterial isolates were cultured and supernatants were collected for the extraction of secondary metabolites using diaion HP-20 to obtain methanol extracts. Evaluation of cytotoxicity property was carried out on macrophage cell lines (RAW264.7) by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazoliumbromide assay. Anti-inflammatory screening was done by inducible nitric oxide assay on RAW264.7 cell lines with lipopolysaccharide (LPS) stimulation. Dianion HP-20 was used to remove salt content. A selected methanol extract was subjected to further fractionations by C-18 reverse phase and their anti-inflammatory potential was evaluated by COX-1 and COX-2, and sPLA2 enzymatic assay.Results: Seven methanol extracts showed no cytotoxic property against RAW 264.7 cell line (inhibitory concentration 50% > 30 µg/ml) and selected for anti-inflammatory screening assay. Result showed methanol extract HM 1.2 reduced NO production >80% and it has been selected for phytochemical screening, further fractionations and assay. Phytochemical screening showed alkaloids and terpenoids present in the HM 1.2. The HM 1.2 and its fractions (F1, F2, F1C1, F1C2, F1C3, and F1C4) were proven to inhibit COX-1, COX-2, and sPLA2 activity in the range of 60.516-116.886%, 20.554-116.457%, and 70.2667-114.8148%, respectively. Conclusions:This study revealed that bacteria associated with H. amboinensis have produced anti-inflammatory activity via reducing NO production and inhibiting COX-1, COX-2, and sPLA2 activity.

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Synergistic interaction between Astragali Radix and Rehmanniae Radix in a Chinese herbal formula to promote diabetic wound healing

Cited by 116 publications

References 38 publications

Bioassay-guided isolation of anti-inflammatory components from the root of Rehmannia glutinosa and its underlying mechanism via inhibition of iNOS pathway

Bioassay-guided isolation of anti-inflammatory components from the root of Rehmannia glutinosa and its underlying mechanism via inhibition of iNOS pathway

Phyto-Extracts in Wound Healing

Anti-Inflammatory Activity of Bacteria Associated With Marine Sponge (Haliclona Amboinensis) via Reducting No Production and Inhibiting Cyclooxygenase-1, Cyclooxygenase-2, and Secretory Phospholipase A2 Activities

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