Synthesis and antibiofilm activity of novel 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides

Abstract

Synthesis of novel alkyl-substituted 4-oxo-1,4-dihydropyrido [1,2-a]pyrrolo [2,3-d]pyrimidine-2-carboxamides and evaluation of their antibiofilm activity in vitro. Methods. Organic synthesis, analytical and spectral methods, broth microilution method, biofilm formation on abiotic surface. Results. A simple and efficient method for the synthesis of new 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxylic acid derivatives was developed. The results of antibiofilm activity screening showed that among the synthesized alkyl-substituted 1,4-dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides there are the compounds capable of disrupting the formation of biofilm of methicillin-resistant strain S. aureus 222, E. coli 311 and P. aeruginosa 449. Compound 6g is active against biofilms of E. coli 311, biomass decreases by 91.2 %, and against S. aureus 222 (reduction by 54.0 %). Compound 6d is active against biofilms of P. aeruginosa 449 and S. aureus 222 (reduction by 78.7 % and 50.2 %, respectively). Conclusions. A series of novel substituted 1-alkyl-4-oxo-1,4dihydropyrido[1,2-a]pyrrolo[2,3-d]pyrimidine-2-carboxamides were synthesized. The acti vity of the synthesized pyrido[1,2-a]pyrrolo [2,3-d]pyrimidines towards the S. aureus 222, E. coli 311 and P. aeruginosa 449 biofilm formation was investigated, and the compounds with pronounced antibiofilm activity were found. K e y w o r d s: pyrido[1,2-a]pyrrolo [2,3-d]pyrimidines, pyrido[1,2-a]pyrimidine-3-carbaldehydes, synthesis, antibiofilm activity.