Synthesis and application of intercellular Ca2+-sensitive fluorescent probe based on quantum dots

Xia, Jinzhi; Yu, Ying; Liao, Qiumei; Cao, Yujuan; Lin, Bixia; Hu, Xiaogang; Wu, Jian‐Zhong

doi:10.1016/j.jinorgbio.2012.09.014

Cited by 17 publications

(12 citation statements)

References 40 publications

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“…The data demonstrated that PHPD could bind preferentially with QDs compared with other QDs binding small molecules, peptides, and proteins in cell lysate. Similar results were also found in other research groups .…”

Section: Separation and Characterization Of Qds Conjugatessupporting

confidence: 92%

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

2014

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Semiconductor quantum dots (QDs) are very important luminescent nanomaterials with a wide range of potential applications. Currently, QDs as labeling probes are broadly used in bioassays, including immunoassay, DNA hybridization, and bioimaging, due to their excellent physical and chemical properties, such as broad excitation spectra, narrow and size-dependent emission profiles, long fluorescence life time, and good photostability. The characterization of QDs and their conjugates is crucial for their wide bioapplications. CE has become a powerful tool for the separation and characterization of QDs and their conjugates. In this review, some CE separation models of QDs are first introduced, mainly including CZE, CGE, MEKC, and ITP. And then, some key applications, such as the measurements of size, surface charge, and concentration of QDs and the characterization of QDs conjugates (e.g. QD-protein, QD-DNA, QD-small molecule), are also described. Finally, future perspectives are discussed.

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Section: Separation and Characterization Of Qds Conjugatessupporting

confidence: 92%

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

2014

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“…Fluorescent dyes1 are vital analytical reagents and are widely used in molecular cell biology. Fluorophores that respond in a well‐defined way by changing their photophysical properties to the change in environmental factors, such as pH,2, 3 temperature,4, 5 gases,6, 7 organic8–10 and inorganic11, 12 analytes, and solvent polarity,13, 14 are of special interest. One of the most important groups is the so‐called solvatochromic dyes, the emission properties (emission maximum, quantum yield, fluorescent lifetime) of which are highly dependent upon the polarity of the surrounding environment.…”

Section: Introductionmentioning

confidence: 99%

Solvatochromic Study of Highly Fluorescent Alkylated Isocyanonaphthalenes, Their π‐Stacking, Hydrogen‐Bonding Complexation, and Quenching with Pyridine

et al. 2014

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Mono- and dialkylated derivatives of 1-amino-5-isocyanonaphthalene (ICAN) were studied as new members of a multifunctional, easy-to-prepare fluorophore family, which showed excellent solvatochromic properties. The monoallyl derivative and the starting ICAN exhibited strong fluorescence quenching in the presence of small amounts of pyridine. The formation of a hydrogen-bonded ground-state pyridine complex was detected; however, analysis of quantum chemical calculations suggested the presence of an additional π-stacked pyridine complex. The Stern-Volmer plot of the quenching process exhibited a downward curvature and after reaching a minimum the fluorescence intensity increased back to a significant level at high pyridine concentrations. Significant fluorescence was observed even in pure pyridine. A new mechanism and a simple mathematical equation were derived to explain the downward curvature and the remaining fluorescence by the formation of a fluorescent π-stacked complex.

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“…The DL of L for Ca 2+ was found as 4.53 × 10 −8 M (R 2 = 0.997) (inset in Figure b) (DL = 3σ/k, in which σ is the standard deviation of the blank solution; and k is the slope of the calibration curve) . Sensor performance was compared with other previously published data for Ca 2+ sensors . As listed in Table , the DL values and linear range from this study approximated to or were better than other reported data.…”

Section: Resultsmentioning

confidence: 50%

Selective sensing Ca²⁺ with a spiropyran‐based fluorometric probe

et al. 2019

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Spiropyran (SP) and its derivatives operate between their ring opening and closing forms as a versatile molecular platform for the fluorescence detection of cations and anions, using a colour change for signalling. A functionalized SP fluorescence probe, L, was prepared and characterized. Probe L can detect Ca 2+ with a fluorescence 'turn-on' response in ethanol solution. It selectively binds Ca 2+ to form a 1:1 ligand/metal complex, which produced a new emission band centred at 604 nm. The sensing result was clearly observed by the solution colour change from colourless to pink under visible light, and from blue to red under ultraviolet light. The detection limit was calculated to be 4.53 × 10 −8 M for Ca 2+ . The probe provides another possibility that SP-based derivatives could be used for the development and detection of metal ions in environmental and physiological systems.

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Synthesis and application of intercellular Ca2+-sensitive fluorescent probe based on quantum dots

Cited by 17 publications

References 40 publications

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

Solvatochromic Study of Highly Fluorescent Alkylated Isocyanonaphthalenes, Their π‐Stacking, Hydrogen‐Bonding Complexation, and Quenching with Pyridine

Selective sensing Ca²⁺ with a spiropyran‐based fluorometric probe

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Synthesis and application of intercellular Ca2+-sensitive fluorescent probe based on quantum dots

Cited by 17 publications

References 40 publications

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

Solvatochromic Study of Highly Fluorescent Alkylated Isocyanonaphthalenes, Their π‐Stacking, Hydrogen‐Bonding Complexation, and Quenching with Pyridine

Selective sensing Ca2+ with a spiropyran‐based fluorometric probe

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Selective sensing Ca²⁺ with a spiropyran‐based fluorometric probe