Synthesis and applications of functionalized magnetic materials in sample preparation

Li, Xiao-Shui; Zhu, Guo‐Qiang; Luo, Yang; Yuan, Bi-Feng

doi:10.1016/j.trac.2012.10.015

Cited by 241 publications

(114 citation statements)

References 150 publications

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“…Generally they should be functionalized with special ligands for target analytes [7,8]. For example, MNPs functionalized with C 18 , C 8 and cyclodextrin were applied for the extraction of organophosphorous pesticides [9], peptides [10] and biphenolic pollutants [11], respectively.…”

Section: Introductionmentioning

confidence: 99%

Magnetic nanoparticles with hydrophobicity and hydrophilicity for solid-phase extraction of polycyclic aromatic hydrocarbons from environmental water samples

Xue

Tang

et al. 2015

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Magnetic nanoparticles with hydrophobicity and hydrophilicity for solid-phase extraction of polycyclic aromatic hydrocarbons from environmental water samples

Xue

Tang

et al. 2015

Journal of Chromatography A

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“…Thus, the study of protein phosphorylation states is important for defining protein kinase substrates, and revealing the activation states of signal transduction pathways. [5][6][7] Currently, high-throughput proteomic analysis is mainly accomplished by mass spectrometry (MS). 8 However, the determination of phosphopeptides in complex biological samples has remained a significant challenge because their scarcity in the total proteome as well as their deficient ionization and ion suppression during the mass spectrometric analysis.…”

Section: Introductionmentioning

confidence: 99%

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

et al. 2017

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The selective isolation of phosphorylated peptides and subsequent analysis using mass spectrometry is important for understanding how protein kinase and phosphatase signals can precisely modulate the on/off states of signal transduction pathways. However, the isolation and detection of multi-phosphorylated peptides is still limited due to their distinct affinity to various materials and their poor ionization efficiency. Here, we report a highly efficient and selective enrichment of phosphorylated peptides using binuclear Zn 2+ -dipicolylamine complex-coated magnetic microspheres (ZnMMs). ZnMMs can utilize the rapid and selective isolation/enrichment of phosphorylated peptides and the subsequent mass spectrometric analysis, given the intrinsic magnetic property of magnetic microspheres and the highly selective binding ability of the binuclear Zn 2+ -dipicolylamine complex to phosphate groups. α-Casein and β-casein were chosen for a proof-of-concept demonstration. We contemplated that phosphopeptides were selectively isolated and enriched from both the tryptic digests of casein proteins and mixed samples with a high degree of sensitivity by facilitating ZnMMs. Especially, ZnMMs showed high efficiency with multi-phosphopeptides, which are in general difficult to be examined by mass analysis on account of their poor ionization efficiency. For the model protein α, β-casein mixture of the tryptic digest, 17 phosphopeptides were identified with ZnMMs and 82% of the enriched phosphopeptides were multiphosphorylated peptides, indicating that ZnMMs have excellent enrichment efficiency and strong affinity towards multiphosphorylated peptides.

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“…Magnetic solids can be coated with antibodies, specific receptors, encapsulating of magnetic particles with biological agents, polymers, and silica modified with different functional groups [28][29][30]. Additional effects of their physical and chemical characteristics must be taken into account during the design of MSPE based methodology.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Solid Phase Extraction Applied to Food Analysis

Ibarra

Rodríguez

Galán-Vidal

et al. 2015

Journal of Chemistry

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Magnetic solid phase extraction has been used as pretreatment technique for the analysis of several compounds because of its advantages when it is compared with classic methods. This methodology is based on the use of magnetic solids as adsorbents for preconcentration of different analytes from complex matrices. Magnetic solid phase extraction minimizes the use of additional steps such as precipitation, centrifugation, and filtration which decreases the manipulation of the sample. In this review, we describe the main procedures used for synthesis, characterization, and application of this pretreatment technique which were applied in food analysis.

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Synthesis and applications of functionalized magnetic materials in sample preparation

Cited by 241 publications

References 150 publications

Magnetic nanoparticles with hydrophobicity and hydrophilicity for solid-phase extraction of polycyclic aromatic hydrocarbons from environmental water samples

Magnetic nanoparticles with hydrophobicity and hydrophilicity for solid-phase extraction of polycyclic aromatic hydrocarbons from environmental water samples

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

Magnetic Solid Phase Extraction Applied to Food Analysis

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