Synthesis and characterisation of fluorescent and biocompatible hydroxyapatite nanoparticles with cerium doping

Lately, there has been an increasing demand for materials that could improve tissue regenerative therapies and provide antimicrobial effects. Similarly, there is a growing need to develop or modify biomaterials for the diagnosis and treatment of different pathologies. In this scenario, hydroxyapatite (HAp) appears as a bioceramic with extended functionalities. Nevertheless, there are certain disadvantages related to the mechanical properties and lack of antimicrobial capacity. To circumvent them, the doping of HAp with a variety of cationic ions is emerging as a good alterative due to the different biological roles of each ion. Among many elements, lanthanides are understudied despite their great potential in the biomedical field. For this reason, the present review focuses on the biological benefits of lanthanides and how their incorporation into HAp can alter its morphology and physical properties. A comprehensive section of the applications of lanthanides-substituted HAp nanoparticles (HAp NPs) is presented to unveil the potential biomedical uses of these systems. Finally, the need to study the tolerable and non-toxic percentages of substitution with these elements is highlighted.

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“…Based on these changes, Ce-doping can be proposed for cell probing and imaging. Similar results were described by Huang et al [ 49 ].…”

Section: Impact Of Lanthanide Incorporation On the Morphology And Pro...supporting

confidence: 92%

Lanthanides-Substituted Hydroxyapatite for Biomedical Applications

Lama-Odría

Valle

Puiggalı́

2023

IJMS

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“…The UV-Vis spectra of Ca 10-x Ce(III)x(PO 4 ) 6 (OH) 2 has maxima which covers a wavelength range from 364 to 378 nm, and for Ca 10-x Ce(IV)x(PO 4 ) 6 (OH) 2 from 343 to 361 nm, respectively. The intensities of this maxima increase with the increasing cerium content as Ce ions display strong UV absorbtion [ 57 ]. Pure hydroxyapatite presents no absorbtion band, therefore the UV absorbtion band of the doped powders can be attributed to the presence of Ce ions.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of Photoluminescent Ce(III) and Ce(IV) Substituted Hydroxyapatite Nanomaterials by Co-Precipitation Method: Cytotoxicity and Biocompatibility Evaluation

et al. 2021

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Improved compounds of Ce(III) and Ce(IV)-doped hydroxyapatite (Ca10-xCex(PO4)6(OH)2) with different concentrations such as x = 0.5, 1, 2.5, 5, and 10%, obtained by the simple co-precipitation method were synthesized. The cerium (3+) and cerium (4+)-doped hydroxyapatite were evaluated for biocompatibility and fluorescence properties. It was found that the cerium-HAp powders were non-toxic, even at higher level of concentration. The synthesized powders were further characterized by FTIR spectrometry, UV-Vis spectroscopy, XRD diffraction, SEM and TEM analysis. Therefore, the present study proves that the developed cerium (3+) and cerium (4+)-doped hydroxyapatite, respectively can be widely used as luminescent labeling materials, with improved biological properties.

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“…Cytotoxicity was encountered only for Ce-HA-NPs concentrations in the range of 800–1000 µg mL −1 [348]. Also, although a slight decrease in cell viability was observed in comparison to the control, the CCK-8 assay indicated no significant cytotoxicity against mouse L929 fibroblast cells, when a Ce-doped (5 at.%) HA powder prepared by co-precipitation was added to the medium at doses lower than 100 µg mL −1 [386]. Slight cytotoxicity was observed for doses of 200 and 500 µg mL −1 [386].…”

Section: Cation-substituted Hydroxyapatitesmentioning

confidence: 99%

“…Also, although a slight decrease in cell viability was observed in comparison to the control, the CCK-8 assay indicated no significant cytotoxicity against mouse L929 fibroblast cells, when a Ce-doped (5 at.%) HA powder prepared by co-precipitation was added to the medium at doses lower than 100 µg mL −1 [386]. Slight cytotoxicity was observed for doses of 200 and 500 µg mL −1 [386]. In contrast, in vitro cytotoxicity assessed by MTT against human lung A549 cells exposed to Ce-doped (10 at.%) HA-NPs concentrations of 100 µg mL −1 showed a significant decrease in cell viability [344].…”

Section: Cation-substituted Hydroxyapatitesmentioning

confidence: 99%

Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods

et al. 2018

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High-performance bioceramics are required for preventing failure and prolonging the life-time of bone grafting scaffolds and osseous implants. The proper identification and development of materials with extended functionalities addressing socio-economic needs and health problems constitute important and critical steps at the heart of clinical research. Recent findings in the realm of ion-substituted hydroxyapatite (HA) could pave the road towards significant developments in biomedicine, with an emphasis on a new generation of orthopaedic and dentistry applications, since such bioceramics are able to mimic the structural, compositional and mechanical properties of the bone mineral phase. In fact, the fascinating ability of the HA crystalline lattice to allow for the substitution of calcium ions with a plethora of cationic species has been widely explored in the recent period, with consequent modifications of its physical and chemical features, as well as its functional mechanical and in vitro and in vivo biological performance. A comprehensive inventory of the progresses achieved so far is both opportune and of paramount importance, in order to not only gather and summarize information, but to also allow fellow researchers to compare with ease and filter the best solutions for the cation substitution of HA-based materials and enable the development of multi-functional biomedical designs. The review surveys preparation and synthesis methods, pinpoints all the explored cation dopants, and discloses the full application range of substituted HA. Special attention is dedicated to the antimicrobial efficiency spectrum and cytotoxic trade-off concentration values for various cell lines, highlighting new prophylactic routes for the prevention of implant failure. Importantly, the current in vitro biological tests (widely employed to unveil the biological performance of HA-based materials), and their ability to mimic the in vivo biological interactions, are also critically assessed. Future perspectives are discussed, and a series of recommendations are underlined.

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Synthesis and characterisation of fluorescent and biocompatible hydroxyapatite nanoparticles with cerium doping

Cited by 8 publications

References 32 publications

Lanthanides-Substituted Hydroxyapatite for Biomedical Applications

Lanthanides-Substituted Hydroxyapatite for Biomedical Applications

Synthesis and Characterization of Photoluminescent Ce(III) and Ce(IV) Substituted Hydroxyapatite Nanomaterials by Co-Precipitation Method: Cytotoxicity and Biocompatibility Evaluation

Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods

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