Synthesis and characterization of polyurethane microspheres and their application for immobilization of maltogenase

Budrienė, Saulutė; Romaskevic, Tatjana; Pielichowski, Krzysztof; Pielichowski, Jan

doi:10.1002/pat.797

Cited by 13 publications

(6 citation statements)

References 13 publications

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“…Veselova and Shekhovtsova [6] studied different esters, ethers and their mixtures with PU foam as support for the immobilization of horseradish peroxidase. Phadtare et al [7] constructed a biosensor based on immobilized pepsin on the nano-gold-PU microparticles conjugates (templates), and Budriene et al [8] have synthesized a series of PU microspheres to immobilize maltogenase.…”

Section: Introductionmentioning

confidence: 99%

Carbon paste electrode modified with pine kernel peroxidase immobilized on pegylated polyurethane nanoparticles

Fritzen‐Garcia

Oliveira

Zanetti-Ramos

et al. 2009

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Carbon paste electrode modified with pine kernel peroxidase immobilized on pegylated polyurethane nanoparticles

Fritzen‐Garcia

Oliveira

Zanetti-Ramos

et al. 2009

Sensors and Actuators B: Chemical

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“…Polyketal microparticles were tested for radical scavenging by delivering superoxide dismutase to a model of lung fibrosis, with the ketals displaying no inflammation response compared with the inflammation found using the standard poly(lactic- co -glycolic acid) (PLGA) particles and ultimately inhibiting lung fibrosis . Formation of polyurethane microspheres with varied morphologies and diameters is primarily achieved through solution templating, using a variety of solvents, conditions, and monomers, which yields particles with sizes ranging from below 1 μm to nearly 500 μm. − Poly(urethane urea) shell particles from acetone/water mixtures were selected in this study, based on results from the previous characterization of degradation products from the materials as well as the possibility of loading radical scavenging antioxidants. , …”

Section: Introductionmentioning

confidence: 99%

Polyurethane Microparticles for Stimuli Response and Reduced Oxidative Degradation in Highly Porous Shape Memory Polymers

Weems

Maitland

et al. 2018

ACS Appl. Mater. Interfaces

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Shape memory polymers (SMPs) have been found to be promising biomaterials for a variety of medical applications; however, the clinical translation of such technology is dependent on tailorable properties such as gravimetric changes in degradation environments. For SMPs synthesized from amino-alcohols, oxidation resulting in rapid mass loss may be problematic in terms of loss of material functionality as well as toxicity and cytocompatibility concerns. Control of gravimetric changes was achieved through the incorporation of small molecule antioxidants, either directly into the polymer matrix or included in microparticles to form a SMP composite material. With direct incorporation of small molecule phenolic antioxidant 2,2'-methylenebis(6- tert-butyl)-methylphenol (Methyl), SMPs displayed reduce strain recovery by more than 50% (Methyl) and increase elastic modulus from approximately 1.4 to 2.3 MPa, at the expense of the strain to failure being reduced from 45% to 32%. Importantly, such changes could not ensure retention of the antioxidants and therefore did not increase oxidative stability beyond 15 days in accelerated oxidative conditions (equivalent to approximately 800 days in porcine aneurysms) in all cases except for the inclusion of a hindered amine that capped network growth, which also resulted in shape memory reduction (only 80% recoverable strain achieved). However, the inclusion of antioxidants in microparticles was found to produce materials with similar thermomechanical ( T migration below 1.0 °C) and shape recovery of 100%, while increasing oxidative resistance compared to controls (oxidation onset was delayed by 3 days and material lifespan increased to approximately 20-22 days in accelerated oxidative solution or beyond 1000 days in the porcine aneurysm). The microparticle composite SMPs also act as a platform for environmental sensing, such as pH-dependent fluorescence shifts and payload release, as demonstrated by fluorescent dye studies using phloxine B and nile blue chloride and the release of antioxidants over a 3 week period. The use of polyurethane-urea microparticles in porous SMPs is demonstrated to increase biostability of the materials, by approximately 25%, and ultimately extend their lifespan for use in aneurysm occlusion as determined through calculated in vivo degradation rates corresponding to a porcine aneurysm environment.

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“…Our research group has explored the immobilization route of BsMa onto PU and PUU microparticles by covalent attachment when enzyme functional groups reacted with free isocyanate groups of the carrier [26][27][28]. Except our own research, we are not aware of other studies of BsMa immobilization onto porous PUU microparticles, which report properties of the porosity of carrier [29].…”

Section: Introductionmentioning

confidence: 99%

Porous poly(urethane urea) microparticles for immobilization of maltogenic α amylase from Bacillus stearothermophilus

Strakšys¹,

Kochanė²,

Maciulyte³

et al. 2021

chemija

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Maltogenic α amylase from Bacillus stearothermophilus (BsMa) was immobilized by covalent attachment and physical adsorption onto porous poly(urethane urea) (PUU) microparticles obtained from poly(vinyl alcohol) (PVA) and 4,4’-methylenebis(cyclohexyl diisocyanate) (H12DI) by onepot synthesis. The influence of PUU synthesis parameters such as PVA and H12DI molar ratio, synthesis time and temperature on porosity, surface area, structure of microparticles and catalytic activity and stability of immobilized BsMa was investigated. The highest efficiency of the immobilization of BsMa onto the PUU carrier was 97% and the highest residual stability of the immobilized enzyme reached 95% after 28 days of storage at 4°C. The optimal activity temperature of immobilized BsMa was at 80°C and it was higher than that of native enzyme. Effects of ionic strength and repetitive batch processing cycles on the activity of immobilized BsMa were also studied. Immobilization of BsMa onto PUU carriers has a great potential for biotechnology and food industries.

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Synthesis and characterization of polyurethane microspheres and their application for immobilization of maltogenase

Cited by 13 publications

References 13 publications

Carbon paste electrode modified with pine kernel peroxidase immobilized on pegylated polyurethane nanoparticles

Carbon paste electrode modified with pine kernel peroxidase immobilized on pegylated polyurethane nanoparticles

Polyurethane Microparticles for Stimuli Response and Reduced Oxidative Degradation in Highly Porous Shape Memory Polymers

Porous poly(urethane urea) microparticles for immobilization of maltogenic α amylase from Bacillus stearothermophilus

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