“…This showed that the antigenicity of the purified LPS and OSP was maintained. These results are in accordance with previous observations of antigenicity of LPS and OSP (Chu et al 1991; Ali et al 2014). Thus the purified OSP of the local isolate of S .…”
Section: Discussionsupporting
confidence: 94%
“…The purified LPS were electrophoresed on SDS-PAGE (Fig. 2 lanes 2–5) and showed characteristic ladder like pattern of S. Paratyphi A LPS as previously reported (Ali et al 2014). Thus procedure adopted here can be scaled up to yield important enteric antigens for subsequent use in vaccine preparation.Fig.…”
Section: Resultssupporting
confidence: 55%
“…In a previous report S . Paratyphi A isolated from China was conjugated with a carrier protein (Ali et al2014). …”
BackgroundParatyphoid fever caused by Salmonella enterica serovar Paratyphi A is becoming a serious health problem in Asian countries particularly Pakistan, China and India and situation is aggravated by current unavailability of a licensed vaccine. This study was designed to purify the O-specific polysaccharides (OSP) produced by an isolate of Salmonella Paratyphi A from Pakistan and detect antigenicity of extracted lipopolysaccharide (LPS) and purified OSP pioneerly in South Asian region as candidate for conjugate vaccine preparation.Results
S. Paratyphi A isolates were identified through PCR using primers of fliC-a gene (329 bp) and confirmed via nested PCR using fliC-nested primers (289 bp). Yield of the LPS of S. Paratyphi A isolate was 40 mg/L of the bacterial culture using hot phenol method. The purified LPS revealed the characteristic ladder like pattern of S. Paratyphi A LPS on SDS-PAGE with silver staining. Purified OSP obtained by acid hydrolysis yielded 23 mg/L of culture broth and was not detected by silver staining. Antigenic interaction of the purified LPS and OSP with hyper immune mice sera was confirmed by single precipitin line evaluated through immunodiffusion assay. The antigenicity was found well intact.ConclusionsThe purified antigenic OSP from S. Paratyphi A may have the potential to be coupled with a carrier protein to develop low cost conjugate vaccine candidates against S. Paratyphi A in paratyphoid endemic regions.
“…This showed that the antigenicity of the purified LPS and OSP was maintained. These results are in accordance with previous observations of antigenicity of LPS and OSP (Chu et al 1991; Ali et al 2014). Thus the purified OSP of the local isolate of S .…”
Section: Discussionsupporting
confidence: 94%
“…The purified LPS were electrophoresed on SDS-PAGE (Fig. 2 lanes 2–5) and showed characteristic ladder like pattern of S. Paratyphi A LPS as previously reported (Ali et al 2014). Thus procedure adopted here can be scaled up to yield important enteric antigens for subsequent use in vaccine preparation.Fig.…”
Section: Resultssupporting
confidence: 55%
“…In a previous report S . Paratyphi A isolated from China was conjugated with a carrier protein (Ali et al2014). …”
BackgroundParatyphoid fever caused by Salmonella enterica serovar Paratyphi A is becoming a serious health problem in Asian countries particularly Pakistan, China and India and situation is aggravated by current unavailability of a licensed vaccine. This study was designed to purify the O-specific polysaccharides (OSP) produced by an isolate of Salmonella Paratyphi A from Pakistan and detect antigenicity of extracted lipopolysaccharide (LPS) and purified OSP pioneerly in South Asian region as candidate for conjugate vaccine preparation.Results
S. Paratyphi A isolates were identified through PCR using primers of fliC-a gene (329 bp) and confirmed via nested PCR using fliC-nested primers (289 bp). Yield of the LPS of S. Paratyphi A isolate was 40 mg/L of the bacterial culture using hot phenol method. The purified LPS revealed the characteristic ladder like pattern of S. Paratyphi A LPS on SDS-PAGE with silver staining. Purified OSP obtained by acid hydrolysis yielded 23 mg/L of culture broth and was not detected by silver staining. Antigenic interaction of the purified LPS and OSP with hyper immune mice sera was confirmed by single precipitin line evaluated through immunodiffusion assay. The antigenicity was found well intact.ConclusionsThe purified antigenic OSP from S. Paratyphi A may have the potential to be coupled with a carrier protein to develop low cost conjugate vaccine candidates against S. Paratyphi A in paratyphoid endemic regions.
“…The studies for analysis of growth pattern analysis were also carried out (20,21). A fresh colony of bacteria was inoculated into SCD media.…”
Section: Methodsmentioning
confidence: 99%
“…The escalating rate of paratyphoid infections appears as a budding problem which requires the advancement in diagnostic techniques as well as effective vaccines for Salmonella enterica serovar Paratyphi A to deal with emerging cases of infections. 21.6 million illnesses and 216,500 deaths globally in 2000, and based on one case of paratyphoid fever for every four cases of typhoid fever, S. paratyphi A is estimated to have caused an additional 5.4 million illnesses in 2000. These estimates, however, are known to vary widely between regions while the cases of enteric fever caused by S. paratyphi A are still increasing (16).…”
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