Synthesis, biological evaluation and cellular localization study of fluorescent derivatives of Jiyuan Oridonin A

Zhou, Chen; Zhang, Junyuan; Liu, Hanbo; Tian, Xiangyu; Liu, Yue; Wang, Yalei; Zheng, Zijun; Wang, Ni; Wang, Zhijia; Xu, Xia; Yu, Ke

doi:10.1016/j.ejmech.2021.114048

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…7a). 34 The hemiacetal hydroxyl group at this position between the A- and B-rings of 25 seemingly has little effect on the overall activity because it is distant from the active center. Qualitative and quantitative studies of cellular uptake revealed that probe 29 rapidly entered HGC-27 cells in a concentration- and time-dependent manner.…”

Section: Subcellular Localization and Target Validationmentioning

confidence: 99%

Natural-product-based fluorescent probes: recent advances and applications

Sung

Lee

2023

RSC Med. Chem.

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Section: Subcellular Localization and Target Validationmentioning

confidence: 99%

Natural-product-based fluorescent probes: recent advances and applications

Sung

Lee

2023

RSC Med. Chem.

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“…In addition, the preventive and therapeutic effects of ORI were revealed on the nude mice hemi-spleen model of colon cancer liver metastasis (Zhou et al, 2022). ORI induced oxidative stress-mediated cancer cells apoptosis by targeting thioredoxin reductase (Li et al, 2021b;Zhu et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Oridonin protects hydrogen peroxide-induced human lens epithelial cell damage by regulating the NLRP3/NF-κB pathway and Nrf2-mediated oxidative stress

Chen

Zeng

Xiao

et al. 2023

Qual. Assur. Saf. Crops Foods

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Cataract is the clouding of eye lens, and is the leading cause of visual impairment and blindness worldwide. There is an urgent need to develop new drugs to combat this disease. Oridonin (ORI), isolated from Rabdosia rubescens, is a natural substance that has been studied as an activator of nuclear factor erythroid 2-related factor 2 (Nrf2) and covalent inhibitor of NLR family pyrin domain containing 3 (NLRP3). Whether ORI has a therapeutic effect on human lens’ epithelial cell injury needs further study. In this study, we used cataract lens epithelial cell lines HLE-B3 and SRA01/04. We found using the MTT assay that ORI treatment increased cell viability induced by hydrogen peroxide (H2O2). In addition, ORI suppressed the apoptosis of H2O2-induced HLE-B3 and SRA01/04 cells detected by flow cytometry and Western blot analysis. Our data further revealed that ORI regulated nuclear factor, erythroid 2 (NFE2) like bZIP transcription factor 2 (Nrf2)-mediated oxidative stress by enzyme-linked-immunosorbent serologic assay. We further found that ORI inhibited NLRP3/nuclear factor-κb (NF-κB) pathway in H2O2-induced HLE-B3 and SRA01/04 cells by Western blot analysis. Therefore, these results suggested that ORI could have the potential to serve as a promising drug to treat cataract.

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