Synthesis, characterization and anticancer activity of vincristine loaded folic acid-chitosan conjugated nanoparticles on NCI-H460 non-small cell lung cancer cell line

Kumar, Naresh; Salar, Raj Kumar; Prasad, Minakshi; Ranjan, Koushlesh

doi:10.1016/j.ejbas.2017.11.002

Cited by 36 publications

(21 citation statements)

References 27 publications

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“…The DEX encapsulation of SPIONs provides desirable stability with no toxicity reported [ 11 , 12 , 13 ]. Besides that, an efficient nanostructured framework is provided for potential treatment delivery to the target [ 14 ]. These systems enable local medication to be administered extensively and provide an improvement in medication concentrations within cancer cells, including moderate and minimum bloodstream concentrations of the drug [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors

Al-Musawi¹,

Ibraheem

Mahdi³

et al. 2021

Life

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Background: Advanced nanobiotechnology provides safe and efficient drug delivery systems to deliver chemotherapy that targets cancer cells efficiently. Methods: A polymeric-magnetic nanocarrier was composed of a dextran (DEX) shell, a superparamagnetic iron oxide (SPION) core and was conjugated with folate (FA) to carry the anticancer drug vincristine (VNC) in Tera-1 testicular tumor cells. The molecular mechanisms by which apoptosis was induced were analyzed using flow cytometry and qPCR, which exhibited anticancer activity of nanoparticles (NPs). Results: This nanocarrier revealed a controlled release of VNC in citrate and phosphate buffer solutions that were maintained at pH 5.5 and pH 7.4, respectively. The Inhibitory concentration (IC50) values were greater than 5 mg/mL and displayed ten times higher cytotoxicity than the comparable free drug concentration. The Caspase-9 and P53 expressions were increased, whereas P21 and AKt1 decreased noticeably in the treated cells. The results point to the possible activation of apoptosis following treatment with NPs loaded with vincristine.

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Section: Introductionmentioning

confidence: 99%

Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors

Al-Musawi¹,

Ibraheem

Mahdi³

et al. 2021

Life

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“…In fact, because of their nanosized and chemical versatility, chemically engineered tissue-targeted ChNPs can readily cross cell membranes, conductive tissues (such as blood and lymphatic vessels), and the brain barrier [10]. In addition, ChNPs have been shown to exert antitumor activity, either by boosting the body’s immune system or by interfering with the tumor growth [11,12].…”

Section: Introductionmentioning

confidence: 99%

Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model

et al. 2019

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The use of chitosan nanoparticles (ChNPs) in various biological and environmental applications is attracting great interest. However, potential side effects related to ChNP toxicity remain the major limitation hampering their wide application. For the first time, we investigate the potential organ-specific (cardiac, hepatic, and neuromuscular) toxicity of ChNPs (size 100–150 nm) using the zebrafish embryo model. Our data highlight the absence of both acute and teratogenic toxic effects of ChNPs (~100% survival rate) even at the higher concentration employed (200 mg/L). Although no single sign of cardiotoxicity was observed upon exposure to 200 mg/L of ChNPs, as judged by heartbeat rate, the corrected QT interval (QTc, which measures the time between the start of the Q wave and the end of the T wave in the heart's electrical cycle), maximum cardiac arrest, and ejection fraction assays, the same dosage elicited the impairment of both liver size (decreased liver size, but without steatosis and lipid yolk retention) and neurobehavioral activity (increased movement under different light conditions). Although the observed toxic effect failed to affect embryo survival, whether a prolonged ChNP treatment may induce other potentially harmful effects remains to be elucidated. By reporting new insights on their organ-specific toxicity, our results add novel and useful information into the available data concerning the in vivo effect of ChNPs.

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“…Acetogenin selectivity for tumor cells can be explained both by the higher activities of NADH oxidase and the ATP demand that are peculiar to tumor cells and inherent due to their uncontrolled growth. [13] The previous studies reported that there are two main compounds of annonaceous acetogenins using HPLC analysis; they are 12, 15-cis-squamostatin-A and bullatacin. [14] They also showed anticancer properties against various tumor cell lines in evaluation in vitro .…”

Section: Discussionmentioning

confidence: 99%

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells

Fadholly

Proboningrat

Iskandar

et al. 2019

J Adv Pharm Technol Res

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This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC50, 2IC50, and 4IC50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.

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Synthesis, characterization and anticancer activity of vincristine loaded folic acid-chitosan conjugated nanoparticles on NCI-H460 non-small cell lung cancer cell line

Abstract: 2018) Synthesis, characterization and anticancer activity of vincristine loaded folic acid-chitosan conjugated nanoparticles on NCI-H460 non-small cell lung cancer cell line,

Cited by 36 publications

References 27 publications

Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors

Smart Nanoformulation Based on Polymeric Magnetic Nanoparticles and Vincristine Drug: A Novel Therapy for Apoptotic Gene Expression in Tumors

Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells

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