Synthesis, Characterization, and Application of Antibody Functionalized Fluorescent Silica Nanoparticles

Hurley, Matthew T.; Wang, Zifan; Mahle, Amanda; Rabin, David; Liu, Qing; English, Douglas S.; Zachariah, Michael R.; Stein, Daniel C.; DeShong, Philip

doi:10.1002/adfm.201202699

Cited by 25 publications

(19 citation statements)

References 57 publications

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“…Effective sensors for glucose, H 2 O 2 , NO 2 , ATP or neurotransmitters were generated by immobilization of sensor molecules on mesoporous silica materials [ 131 , 132 , 133 , 134 , 135 , 136 ]. The various possibilities for functionalization of mesoporous silica materials also enable development of further diagnostic or imaging applications [ 114 , 137 ].…”

Section: Biotechnological Applications Of Silicamentioning

confidence: 99%

Silaffins in Silica Biomineralization and Biomimetic Silica Precipitation

2015

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Biomineralization processes leading to complex solid structures of inorganic material in biological systems are constantly gaining attention in biotechnology and biomedical research. An outstanding example for biomineral morphogenesis is the formation of highly elaborate, nano-patterned silica shells by diatoms. Among the organic macromolecules that have been closely linked to the tightly controlled precipitation of silica in diatoms, silaffins play an extraordinary role. These peptides typically occur as complex posttranslationally modified variants and are directly involved in the silica deposition process in diatoms. However, even in vitro silaffin-based peptides alone, with and without posttranslational modifications, can efficiently mediate biomimetic silica precipitation leading to silica material with different properties as well as with encapsulated cargo molecules of a large size range. In this review, the biomineralization process of silica in diatoms is summarized with a specific focus on silaffins and their in vitro silica precipitation properties. Applications in the area of bio- and nanotechnology as well as in diagnostics and therapy are discussed.

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Section: Biotechnological Applications Of Silicamentioning

confidence: 99%

Silaffins in Silica Biomineralization and Biomimetic Silica Precipitation

2015

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“…For example, monoclonal antibodies (mAb) can be conjugated to the surface of NPs to detect specific biomarkers or receptors expressed on the cell surface. Various fluorescent materials such as fluorescein isothiocyanate (FITC) or green fluorescent protein (GFP) have been employed to encapsulate the NPs for imaging purpose [ 28 ].…”

Section: In Vitro Models For Validating the Functionality Of Npsmentioning

confidence: 99%

Priming nanoparticle-guided diagnostics and therapeutics towards human organs-on-chips microphysiological system

et al. 2016

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Nanotechnology and bioengineering have converged over the past decades, by which the application of multi-functional nanoparticles (NPs) has been emerged in clinical and biomedical fields. The NPs primed to detect disease-specific biomarkers or to deliver biopharmaceutical compounds have beena validated in conventional in vitro culture models including two dimensional (2D) cell cultures or 3D organoid models. However, a lack of experimental models that have strong human physiological relevance has hampered accurate validation of the safety and functionality of NPs. Alternatively, biomimetic human “Organs-on-Chips” microphysiological systems have recapitulated the mechanically dynamic 3D tissue interface of human organ microenvironment, in which the transport, cytotoxicity, biocompatibility, and therapeutic efficacy of NPs and their conjugates may be more accurately validated. Finally, integration of NP-guided diagnostic detection and targeted nanotherapeutics in conjunction with human organs-on-chips can provide a novel avenue to accelerate the NP-based drug development process as well as the rapid detection of cellular secretomes associated with pathophysiological processes.

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“…However, the low concentration of biomarkers in urine begs the need for highly sensitive devices for accurate detection. To detect low concentrations, biosensors often employ complex features such as nanoparticles [1][2][3] and structured surfaces [4][5][6] with equipment intensive amplifi cation methods such as surface plasmon resonance [7][8][9] to enhance the detection signal. Another approach for enhanced detection is increasing the concentration of biomolecules to a range detectable using standard techniques (such as a colorimetric output).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Detection of Protein in Urine by Droplet Evaporation on a Superhydrophobic Plastic

McLane

Khine

2014

Adv Materials Inter

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than wet the surface [10][11][12][13][14][15][16][17][18][19][20][21][22][23] due to the high surface tension of water, the low surface energy of the substrate, and the minimal adhesion between water and the surface. The low surface energy and minimal adhesion can be attributed to multiscale features, ranging from micro to nano, [ 11 ] that trap air pockets between the surface and water, allowing the water droplet to only contact the peaks of the multiscale structures. The multiscale features can be highly patterned (homogeneous) or random (heterogeneous) but need to be multi-to nanoscale to trap air between the surface and fl uid. Such SH surfaces have been used for anti-fouling applications and drug delivery. [ 20,[24][25][26][27] When air is trapped between water and the surface, the surface is in the Cassie-Baxter regime, [ 10 ] and the water droplet's adhesion to the surface is poor. A droplet in the Cassie-Baxter regime can transition to the Wenzel regime (no air pockets and strong adhesion to the surface) [ 28 ] when the balance of forces is disrupted. [ 29 ] Pressure can disrupt this balance and change a water droplet from balancing on the peaks (Cassie) to sinking into the multiscale structures (Wenzel). [30][31][32][33] A water droplet can naturally transition from Cassie to Wenzel due to a change in internal droplet pressure, which can be quantifi ed bywhere γ is the surface tension of the fl uid, and R is the radius of the droplet. [ 30,34 ] Evaporation of a droplet can cause the Cassie to Wenzel transition due to the decrease in volume and increase in internal pressure. When a droplet of fl uid evaporates into the atmosphere, the balance of forces at the air-liquid interface constantly increases the droplet's surface tension and applies an inward force at the droplet's contact line (air-liquid-solid interface). [ 35 ] On a SH surface, the droplet's inward pulling force is greater than the droplet's adhesion to the surface, and the droplet's contact line continually moves inward, creating a smaller footprint and contact diameter (CD) for the droplet, [36][37][38][39][40][41] as shown in Figure 1 . Eventually, the droplet collapses to its fi nal footprint when the internal Laplace pressure overcomes the upward force from the air pockets, [ 30,31 ] and fl uid will stay pinned at this reduced contact area until evaporation is complete. This reduced contact area contains the content of the droplet with the molecules concentrated only on a small footprint.Protein in urine can be detected using a simple colorimetric output by evaporating droplets on a superhydrophobic (SH) surface. Evaporation on a SH surface allows fl uid to dramatically concentrate; the weak surface adhesion allows the droplet of fl uid to constantly decrease its footprint area and contact diameter. On a SH surface, pure water completely evaporates. Molecules in solution, however, are confi ned to a footprint that is 8.5 times smaller than the original and are greatly concentrated. By concentrating molecules, a 160 times improved detectio...

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Synthesis, Characterization, and Application of Antibody Functionalized Fluorescent Silica Nanoparticles

Cited by 25 publications

References 57 publications

Silaffins in Silica Biomineralization and Biomimetic Silica Precipitation

Silaffins in Silica Biomineralization and Biomimetic Silica Precipitation

Priming nanoparticle-guided diagnostics and therapeutics towards human organs-on-chips microphysiological system

Enhanced Detection of Protein in Urine by Droplet Evaporation on a Superhydrophobic Plastic

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