Synthesis, characterization and comparative anticancer potential of phytosynthesized mono and bimetallic nanoparticles using Moringa oleifera aqueous leaf extract.

Abstract

In this article we bring up facile one-step phytosynthesis of Silver (Ag), Gold (Au) and Ag/Au bimetallic nanoparticles by reduction of Silver nitrate and Tetrachloroauric acid solution, using aqueous leaf extract of Moringa oleifera. Physical characterization was done using different techniques including UV-Visible spectroscopy, FT-IR Spectroscopic Analysis, DLS, Zeta Potential, XRD, TEM and EDAX. These nanoparticles were evaluated for their cytotoxicity against human hepatocellular carcinoma cells (HepG2) and breast cancer cell line (MDA-MB-231 and MCF-7). Our data suggest these phytosynthesized nanoparticles showed a dose-dependent cytotoxic effect on all the cancer cell lines. However, AuNPs is seen to have higher cytotoxic potential with IC50 value in the range of 9.20-21.46µg/ml compared to that of Bimetallic NPs (Ag-Au NPs) with IC50 value of 37.22-49.94µg/ml. Whereas, Silver NPs (AgNPs) didn’t show cytotoxic activity upto 60µg/ml in all the three cell lines for 24hrs. Hence, this study supports the effectiveness of phytosynthesized AuNPs for the development of anticancer agents.

Anticancer Potential of Biosynthesized Silver Nanoparticles: A Review

“…Ag, Au and Ag-Au nanoparticles were synthesized using the aqueous leaf extract of Moringa oleifera. The results exhibited the effectiveness of the Ag-Au nanoparticles against the Hep2 cell line (Gupta et al, 2020). Bimetallic nanoparticles are making their way into the diagnostic and therapeutic sciences, thus making them extremely ideal theranostic agents and are also being studied for various biomedical applications that include drug delivery, antimicrobial property, antifungal nature, larvicidal nature amongst others.…”

“…Silver (Ag) and gold (Au) nanoparticles have been thoroughly explored by researchers for their wide range of biomedical and theranostic applications including anti-cancer, anti-microbial, anti-fungal, and antioxidant activities. A combination of the two to form bimetallic nanoparticles has been known to have better biocompatibility and unique properties as compared to that arising from both noble metals individually (Gupta et al, 2020). In the case of Ag-Au nanoparticles, the surface plasmon resonance levels can be altered according to the required structural characteristics just by controlling the Ag: Au ratio in the displacement reaction taking place during the synthesis of this bimetallic structure.…”

“…These nanoparticles also showed blood anticoagulation properties as well as thrombolytic potential making them suitable for the management of blood coagulation disorders (Lateef et al, 2016). Gupta et al (2020), drew a comparative study of the potential anticancer properties of photosynthesized mono and bimetallic nanoparticles. Ag, Au and Ag-Au nanoparticles were synthesized using the aqueous leaf extract of Moringa oleifera.…”

Comparative analysis of the insecticidal activity against Sitophilus oryzae (L.) and agro-morphological characteristics of maize using non-biogenic and biogenic ZnO nanoparticles

“…After cooling, the extract was filtered through Whatman filter paper number 1 and then stored at 40 °C. 14 Zinc acetate solution (2 M) was prepared in 50 mL of deionized water under constant stirring. After the complete dissolution of the mixture, 10 mL of Moringa leaf extract and 50 mL of NaOH (2 M) were poured into the prepared solution of zinc acetate.…”

Green synthesis of biocompatible core–shell (Au–Ag) and hybrid (Au–ZnO and Ag–ZnO) bimetallic nanoparticles and evaluation of their potential antibacterial, antidiabetic, antiglycation and anticancer activities

“…6 BNPs have demonstrated a potential to treat a wide range of cancers including ovarian, lung, oral, breast and many others. 7–9 Other promising application of BNPs include their anti-diabetic and anti-aging properties. 10,11 The advantage of using green BNPs is their target specificity and biocompatibility due to phytochemical capping.…”