Synthesis, Characterization, and In Vivo Distribution of 99mTc Radiolabelled Docetaxel Loaded Folic Acid-Thiolated Chitosan Enveloped Liposomes

Ahmed, Naveed; Sohail, Muhammad; Khurshid, Zain; Ammar, Ayesha; Saeed, Adnan Muhammad; Shazia, Fatima; Shahnaz, Gul; Zia, Muhammad

doi:10.1007/s12668-022-01053-2

Cited by 1 publication

(2 citation statements)

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“…In contrast to previously reported DTX-loaded chitosome synthesis protocols [ 23 , 24 , 38 , 39 , 43 , 47 , 49 , 53 ], our methodology modified a one-step liposome synthesis method that was previously used in our laboratory [ 28 ] and to synthesize DTX-loaded liposomes [ 72 , 80 ]. Briefly, DSPC, DSPE-PEG2000, and cholesterol with a molar ratio of 2/0.18/1 were dissolved in 2 mL of 100% ethanol by gentle stirring.…”

Section: Methodsmentioning

confidence: 99%

“…While several studies have investigated the use of DTX-loaded chitosomes in breast cancer [ 38 , 47 , 49 ], none have investigated the use of such a drug delivery system in head and neck cancer. Additionally, existing DTX-loaded chitosomes are primarily designed for oral ingestion or intravenous injection [ 53 ]. However, as mentioned above, systemic intravenous [ 6 , 7 , 11 , 12 , 13 ] leads to unwanted high toxicities and extravasation.…”

Section: Introductionmentioning

confidence: 99%

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Chitosomes Loaded with Docetaxel as a Promising Drug Delivery System to Laryngeal Cancer Cells: An In Vitro Cytotoxic Study

Moya-Garcia

Li‐Jessen

Tabrizian

2023

IJMS

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Current delivery of chemotherapy, either intra-venous or intra-arterial, remains suboptimal for patients with head and neck tumors. The free form of chemotherapy drugs, such as docetaxel, has non-specific tissue targeting and poor solubility in blood that deters treatment efficacy. Upon reaching the tumors, these drugs can also be easily washed away by the interstitial fluids. Liposomes have been used as nanocarriers to enhance docetaxel bioavailability. However, they are affected by potential interstitial dislodging due to insufficient intratumoral permeability and retention capabilities. Here, we developed and characterized docetaxel-loaded anionic nanoliposomes coated with a layer of mucoadhesive chitosan (chitosomes) for the application of chemotherapy drug delivery. The anionic liposomes were 99.4 ± 1.5 nm in diameter with a zeta potential of −26 ± 2.0 mV. The chitosan coating increased the liposome size to 120 ± 2.2 nm and the surface charge to 24.8 ± 2.6 mV. Chitosome formation was confirmed via FTIR spectroscopy and mucoadhesive analysis with anionic mucin dispersions. Blank liposomes and chitosomes showed no cytotoxic effect on human laryngeal stromal and cancer cells. Chitosomes were also internalized into the cytoplasm of human laryngeal cancer cells, indicating effective nanocarrier delivery. A higher cytotoxicity (p < 0.05) of docetaxel-loaded chitosomes towards human laryngeal cancer cells was observed compared to human stromal cells and control treatments. No hemolytic effect was observed on human red blood cells after a 3 h exposure, proving the proposed intra-arterial administration. Our in vitro results supported the potential of docetaxel-loaded chitosomes for locoregional chemotherapy delivery to laryngeal cancer cells.

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