Synthesis, characterization and osteoconductivity properties of bone fillers based on alendronate-loaded poly(ε-caprolactone)/hydroxyapatite microspheres

Chen, Jianhong; Luo, Yun; Hong, Lei; Ling, You; Pang, Jun; Fang, Youqiang; Wei, Kun; Gao, Xin

doi:10.1007/s10856-011-4232-8

Cited by 26 publications

(39 citation statements)

References 37 publications

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“…Although our implant exerted its beneficial effects during bone healing, it did not remain as a part of new bone as observed in many of the recent studies that used low biodegradable scaffolds [12,24,34,35]. Zhu et al [34] used 250 mg polylactic acid (PLA) with 50, 100, and 200 mg Sim in a LRBD model in rabbits and showed that 100 mg Sim resulted in superior bone formation compared to the controls after 16 weeks.…”

Section: Discussionmentioning

confidence: 89%

“…Although several investigations showed that many strategies are able to accelerate bone healing and regeneration, actually most of them failed to show the remodeled bone after 8 weeks of injury [21,[35][36][37]. In fact, in those studies, differentiation of the cortex and medulla occurred after 12-16 weeks.…”

Section: Discussionmentioning

confidence: 98%

“…In addition, the outcome of cell therapy is not clear because cell passaging reduces the cell life cycle, resulting in cell aging. Strontium and Bisphosphonates such as alendronate have successfully been used in preventing the osteoporosis; however, these compounds significantly reduce osteoclastogenesis which is not beneficial for bone repair [35,[41][42][43][44]. Sim is a safe agent and could controllably be released from the Gel scaffold to promote osteoinduction [10,11,14,34,45].…”

Section: Discussionmentioning

confidence: 99%

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Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

et al. 2015

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Treatment of large bone defects (LBDs) is technically demanding. Tissue engineering is an option. A bioactive graft may be produced by combining tissue scaffolds and healing promotive factors in order to accelerate bone repair. We investigated the role of Simvastatin (Sim)-embedded porous Gelapin (Gel) scaffold on experimental bone healing. At first, the effectiveness of different concentrations of Gel and Sim powders was investigated in an experimentally induced femoral hole model in rabbits (n = 6) for 30 days. Then bone bioactive grafts were produced by combination of the effective concentrations of Gel, Sim, and Genipin. The bioimplants were subcutaneously tested in a rabbit model (n = 9) to determine their biocompatibility and biodegradability for 10-30 days. Finally, a large radial bone defect model was produced in rabbits (n = 20), and the bioimplants were inserted in the defects. The untreated and autograft-treated bone defects were served as controls. The animals were euthanized after 30 and 60 days of bone injury. The bone samples were evaluated by radiography, three-dimensional CT scan, bone densitometry, histopathology, and nano-indentation. At a concentration of 5 mg/hole, Sim closed the femoral bone holes after 30 days, while in the defect, autograft, and Gel groups, the holes were open. Both the Gel and Gel-Sim scaffolds were biocompatible and biodegradable. Subcutaneously, the Gel-Sim scaffold was replaced with the newly regenerated ectopic bone after 30 days. After implantation of the Gel-Sim scaffold in the radial bone defects, the scaffold was completely replaced with new woven bone after 30 days which was then matured and remodeled into a cortical bone after 60 days. Sixty days after bone injury, the Gel-Sim-treated defects had significantly higher bone volume, matrix mineralization, elastic modulus, and contact hardness when compared to the controls. The Gel-Sim scaffold may be a suitable option in managing LBDs.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

et al. 2015

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“…The mixture was poured into a glass petri dish and dried under ambient conditions for 24 hours to obtain the composite film. 17,18 In vitro degradation tests…”

Section: Preparation Of Ciprofloxacin-loaded Hap-pcl Nanocomposite Filmmentioning

confidence: 99%

Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants

Nithya¹,

Sundaram²

2015

IJN

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Introduction In recent years there has been a steep increase in the number of orthopedic patients for many reasons. One major reason is osteomyelitis, caused by pyrogenic bacteria, with progressive infection of the bone or bone marrow and surrounding tissues. So antibiotics must be introduced during bone implantation to avoid prolonged infection. Aim The objective of the study reported here was to prepare a composite film of nanocrystalline hydroxyapatite (HAp) and polycaprolactone (PCL) polymer loaded with ciprofloxacin, a frequently used antibiotic agent for bone infections. Methods Nanocrystalline HAp was synthesized by precipitation method using the precursor obtained from eggshell. The nanocomposite film (HAp-PCL-ciprofloxacin) was prepared by solvent evaporation. Drug-release and biodegradation studies were undertaken by immersing the composite film in phosphate-buffered saline solution, while a cytotoxicity test was performed using the fibroblast cell line NIH-3T3 and osteoblast cell line MG-63. Results The pure PCL film had quite a low dissolution rate after an initial sharp weight loss, whereas the ciprofloxacin-loaded HAp-PCL nanocomposite film had a large weight loss due to its fast drug release. The composite film had higher water absorption than the pure PCL, and increasing the concentration of the HAp increased the water absorption. The in vitro cell-line study showed a good biocompatibility and bioactivity of the developed nanocomposite film. Conclusion The prepared film will act as a sustainable bone implant in addition to controlled drug delivery.

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“…Interestingly, AL has a high affinity for hydroxyapatite and could be hybridized together to circumvent the low bioavailability [24,31]. The hybridization markedly increases the loading efficiency of AL and also provides a sustained release for a long period of time [32,33].…”

Section: Introductionmentioning

confidence: 99%

Emulsion cross-linked chitosan/nanohydroxyapatite microspheres for controlled release of alendronate

Liu

et al. 2014

J Mater Sci: Mater Med

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Sustained delivery of growth factors has emerged as an essential requirement for bone tissue engineering applications for the treatment of various kinds of bone defects. Chitosan (CH) has attracted particular attention for drug delivery and bone tissue engineering because of its favorable biocompatibility and biodegradability. In this study, a composite microsphere system containing CH and nanohydroxyapatite (nHA)-alendronate (AL) particles was fabricated by employing both emulsification and cross-linking strategies. The microspheres were characterized for their surface morphology, composition, size distribution, drug loading efficiency and release properties. The results showed that loading efficiency and sustained release of hydrophilic AL were significantly improved, which is ideal for locally sustained release in the bone microenvironment. In vitro osteogenic studies showed that the microspheres could enhance the osteogenic activity of rabbit adipose-derived stem cells. In conclusion, the CH/nHA-AL composite microspheres exhibit promising properties as a candidate for local treatment for bone defects.

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Synthesis, characterization and osteoconductivity properties of bone fillers based on alendronate-loaded poly(ε-caprolactone)/hydroxyapatite microspheres

Cited by 26 publications

References 37 publications

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

Three-Dimensional Porous Gelapin–Simvastatin Scaffolds Promoted Bone Defect Healing in Rabbits

Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants

Emulsion cross-linked chitosan/nanohydroxyapatite microspheres for controlled release of alendronate

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