Synthesis, Characterization, and Selective Delivery of DARPin–Gold Nanoparticle Conjugates to Cancer Cells

Deyev, Sergey M.; Прошкина, Г. М.; Ryabova, A. V.; Tavanti, Francesco; Menziani, Maria Cristina; Eidelshtein, Gennady; Avishai, Gavriel; Kotlyar, Alexander

doi:10.1021/acs.bioconjchem.7b00410

Cited by 39 publications

(33 citation statements)

References 35 publications

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“…Utilization of biocompatible and ecofriendly processes for the production of nanoparticles is of great importance in current nanotechnology research [1]. Nanomaterials have been synthesized by different methods including hard template [2], bacteria [3], fungi [4] and plants [5]. During the synthesis of metallic nanoparticles by chemical methods toxic compounds are often produced which remains adsorbed on the particle surface and has adverse effects on human health.…”

Section: Introductionmentioning

confidence: 99%

Phyto-mediated silver nanoparticles via Mellisa officinalis aqueous and methanolic extracts: synthesis, characterization and biological properties

Nayeri

Mafakheri

Mirhosseini

2020

Preprint

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The aim of the study was to examine the influence of extraction method on the size, shape and morphology of synthesized silver nanoparticles (AgNPs). Silver nanoparticles were prepared by the aqueous and methanolic extracts of lemon balm. The properties of obtained nanoparticles were characterized by UV-Vis, SEM, XRD and FTIR techniques. The UV-Vis spectroscopy confirmed the formation of AgNPs by observing a distinct surface Plasmon resonance band around 450 nm. SEM images showed different shape, size and morphology of AgNPs using two different extracts types. AgNPs derived from the aqueous extract were rod-shaped with a diameter of 19 to 40 nm whereas spherical particles were synthesized by the methanolic extract found smaller with size distribution ranging from 13 to 35 nm. The XRD pattern indicated that AgNPs formed by the reduction of Ag + ions using methanolic extract of M. officinalis were crystal-like in nature. The functional groups of M. officinalis methanolic extract involved in synthesis and stabilization of AgNPs were investigated by FTIR. In addition, AgNPs-containing methanolic extract showed higher antioxidant activity. These particles exhibited remarkable antimicrobial activity against gram positive and negative bacteria and a fungus. The nanoparticles produced by the methanolic extract of the lemon balm showed antioxidant and antimicrobial activity. The production of silver nanoparticles using plant extract is rapid, low cost and eco-friendly. Therefore, green chemistry is a good alternative to industrial production of nanoparticles. Silver nanoparticles can be used as an antiseptic to sterilize the surrounding area and the hospital wastes.

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Section: Introductionmentioning

confidence: 99%

Phyto-mediated silver nanoparticles via Mellisa officinalis aqueous and methanolic extracts: synthesis, characterization and biological properties

Nayeri

Mafakheri

Mirhosseini

2020

Preprint

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“…In animal models, far-red fluorescent proteins, such as mCherry, can be applied to allow intravital visualization of a tumor [38]. Cherry and HER2-specific DARPin 9_29 were fused to obtain the recombinant protein DARPin-mCherry, which specifically stains HER2positive cancer cells [39] and is used for the functionalization of nanoparticles [40][41][42][43] as described below.…”

Section: Applications Of Darpins In Cancer Diagnosis and Therapymentioning

confidence: 99%

“…NaYF 4 : Yb 3 + Tm 3 + / NaYF 4 particles emitting ultraviolet radiation when exposed to infrared light were coated with the DARPin-mCherry protein [39], which allows visualization of cancer cells thanks to the far-red fluorescent mCherry module [40]. DARPin 9_29 and the DARPin-mCherry protein containing it were also used to coat 5-nm gold nanoparticles [41] and gold nanorods [42]. DARPin was efficiently coupled with the particle surface to form a crown consisting of approximately 35 protein molecules, thus reducing particle aggregation.…”

Section: Application Of Darpins In Nanoparticle Deliverymentioning

confidence: 99%

“…DARPin was efficiently coupled with the particle surface to form a crown consisting of approximately 35 protein molecules, thus reducing particle aggregation. Notably, DARPin interacted with nanoparticles in a way, leaving its HER2-binding surface free, which ensured selective binding of the resulting nanoparticles to HER2-overexpressing cells [41].…”

Section: Application Of Darpins In Nanoparticle Deliverymentioning

confidence: 99%

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DARPins: Promising Scaffolds for Theranostics

Shilova

Deyev

2019

Acta Naturae

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Monoclonal antibodies are the classical basis for targeted therapy, but the development of alternative binding proteins has made it possible to use non-immunoglobulin proteins as targeting modules. The advantages of DARPins, scaffold proteins based on ankyrin repeats, over antibodies are as follows: small size, stability over a wide range of temperatures and pH values, low aggregation tendency, and ease of production in heterologous expression systems. The differences in the structure of the paratope of DARPin and antibodies broaden the spectrum of target molecules, while the ease of creating hybrid fusion proteins allows one to obtain bispecific and multivalent constructs. In this article, we summarize recent data on the development of therapeutic and imaging compounds based on DARPins.

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“…The IL-37 receptor complex is similar to the IL-18 receptor, another representative of the IL-1 family. IL-18 is one of the key pro-inflammatory cytokines acting as a pathogenetic factor in a number of diseases [41,42]. The IL-18 receptor complex consists of two chains: α (IL-18Rα) and β (IL-18Rβ), each of which has a TIR domain [43].…”

Section: Il-37 Receptor Complex and Signaling Pathwaysmentioning

confidence: 99%

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

Shilovskiy¹,

Dyneva²,

Курбачева³

et al. 2019

Acta Naturae

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Cytokines of the interleukin-1 (IL-1) family play an important role in the realization of the protective functions of innate immunity and are the key mediators involved in the pathogenesis of a wide range of diseases, including various manifestations of allergy. The IL-1 family includes more than 11 members. However, the functions of many of them remain to be elucidated. Recently, new members of the IL-1 family have been discovered. In 2000, several independent research groups reported the discovery of a new interleukin of this family, which was named IL-37, or IL-1F7 (according to the new nomenclature). IL-37 was assigned to the IL-1 family based on its structural similarity with other members of this family. The study of its biological properties showed that its activity changes in inflammatory diseases, such as rheumatoid arthritis, psoriasis, as well as allergic diseases (allergic rhinitis, bronchial asthma, and atopic dermatitis). However, unlike most members of the IL-1 family, IL-37 acts as a negative regulator of inflammation. Activation of IL-37 suppresses inflammation, resulting in the suppression of inflammatory cytokines and chemokines, which in turn prevents infiltration of pro-inflammatory cells, mainly eosinophils and neutrophils. The exact molecular and cellular mechanisms of the anti-inflammatory effect of IL-37 in the development of allergic diseases (AD) have not been fully studied. This review summarizes and analyzes the accumulated experimental data on the role of IL-37 in the pathogenesis of AD, such as allergic rhinitis, bronchial asthma, and atopic dermatitis.

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Synthesis, Characterization, and Selective Delivery of DARPin–Gold Nanoparticle Conjugates to Cancer Cells

Cited by 39 publications

References 35 publications

Phyto-mediated silver nanoparticles via Mellisa officinalis aqueous and methanolic extracts: synthesis, characterization and biological properties

Phyto-mediated silver nanoparticles via Mellisa officinalis aqueous and methanolic extracts: synthesis, characterization and biological properties

DARPins: Promising Scaffolds for Theranostics

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

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