Synthesis, Characterization, Cytotoxic Activity, and Interactions with CT-DNA and BSA of Cationic Ruthenium(II) Complexes Containing Dppm and Quinoline Carboxylates

Abstract

The complexes cis-[Ru(quin)(dppm)2]PF6 and cis-[Ru(kynu)(dppm)2]PF6 (quin = quinaldate; kynu = kynurenate; dppm = bis(diphenylphosphino)methane) were prepared and characterized by elemental analysis, electronic, FTIR, 1H, and 31P{1H} NMR spectroscopies. Characterization data were consistent with a cis arrangement for the dppm ligands and a bidentate coordination through carboxylate oxygens of the quin and kynu anions. These complexes were not able to intercalate CT-DNA as shown by circular dichroism spectroscopy. On the other hand, bovine serum albumin (BSA) binding constants and thermodynamic parameters suggest spontaneous interactions with this protein by hydrogen bonds and van der Waals forces. Cytotoxicity assays were carried out on a panel of human cancer cell lines including HepG2, MCF-7, and MO59J and one normal cell line GM07492A. In general, the new ruthenium(II) complexes displayed a moderate to high cytotoxicity in all the assayed cell lines with IC50 ranging from 10.1 to 36 µM and were more cytotoxic than the precursor cis-[RuCl2(dppm)2]. The cis-[Ru(quin)(dppm)2]PF6 were two to three times more active than the reference metallodrug cisplatin in the MCF-7 and MO59J cell lines.

Selective Anticancer and Antimicrobial Metallodrugs Based on Gold(III) Dithiocarbamate Complexes

“…values were higher than other reported for gold(I) complexes [46,47,65], but similar to the documented interactions for potential drugs based on ruthenium or nickel [66,67]. Therefore, our complexes can be transported by BSA protein throughout the body, one molecule each of BSA according to the results of n parameter (except for C1 or C5, where two molecules of gold complex could be found in the same BSA structure).…”

“…According to the K b values observed in Table 3, there was a moderated interaction force between complex-BSA. These values were higher than other reported for gold(I) complexes [46,47,65], but similar to the documented interactions for potential drugs based on ruthenium or nickel [66,67].…”

Sensitive detection of free bilirubin in blood serum using β-diketone modified europium-doped yttrium oxide nanosheets as a luminescent sensor

“…Consequently, the energy delivered by the ligands to the luminescent center is reduced, due to which the luminescence intensity of Eu( iii ) also decreases. The quenching process can be expressed by the following equation: [C] + n [Q] → [C⋯ n Q] 33,34 (C = nanosheets-tta/PS, or nanosheets-dbt/PS).…”

A dual-targeting ruthenium nanodrug that inhibits primary tumor growth and lung metastasis via the PARP/ATM pathway

“…Using ultraviolet–visible (UV) spectroscopy quenching of 2b, we studied the ability of DNA to bind to complex 2b [ 37 ]. With the addition of circulating tumor DNA (ctDNA) in PBS (pH 7.4), the UV absorption curve of 2b (12.5 μM) in solution decreased (Fig.…”