Synthesis of a carboxymethylated guar gum grafted polyethyleneimine copolymer as an efficient gene delivery vehicle

Jana, Piyali; Sarkar, Kishor; Mitra, Tapas; Chatterjee, Arindam; Gnanamani, A.; Chakraborti, Gopal; Kundu, Patit Paban

doi:10.1039/c5ra23447f

Cited by 22 publications

(15 citation statements)

References 40 publications

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“…A carboxymethylated guar gum-grafted-polyethyleneimine copolymer (CMGG-g-PEI) was reported as an effective gene carrier in another study by Jana et al [ 168 ]. The less toxic profile of CMGG-g-PEI was revealed by cytotoxicity and blood compatibility experiments.…”

Section: Applicationsmentioning

confidence: 99%

Plant-Based Gums and Mucilages Applications in Pharmacology and Nanomedicine: A Review

et al. 2021

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Gums are carbohydrate biomolecules that have the potential to bind water and form gels. Gums are regularly linked with proteins and minerals in their construction. Gums have several forms, such as mucilage gums, seed gums, exudate gums, etc. Plant gums are one of the most important gums because of their bioavailability. Plant-derived gums have been used by humans since ancient times for numerous applications. The main features that make them appropriate for use in different applications are high stabilization, viscosity, adhesive property, emulsification action, and surface-active activity. In many pharmaceutical formulations, plant-based gums and mucilages are the key ingredients due to their bioavailability, widespread accessibility, non-toxicity, and reasonable prices. These compete with many polymeric materials for use as different pharmaceuticals in today’s time and have created a significant achievement from being an excipient to innovative drug carriers. In particular, scientists and pharmacy industries around the world have been drawn to uncover the secret potential of plant-based gums and mucilages through a deeper understanding of their physicochemical characteristics and the development of safety profile information. This innovative unique class of drug products, useful in advanced drug delivery applications, gene therapy, and biosynthesis, has been developed by modification of plant-based gums and mucilages. In this review, both fundamental and novel medicinal aspects of plant-based gums and mucilages, along with their capacity for pharmacology and nanomedicine, were demonstrated.

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Section: Applicationsmentioning

confidence: 99%

Plant-Based Gums and Mucilages Applications in Pharmacology and Nanomedicine: A Review

et al. 2021

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“…Although b-PEI has shown to be an excellent transfection vector, it has some limitations, among which are its cytotoxicity, low biodegradability, and low specificity, which gives rise to non-specific interactions with normal cells or blood components at the in vivo level, and this causes a decrease in the half-life of the PEI / DNA polyplexes in the bloodstream [ 20 ]. In order to overcome these limitations, some studies have reported the modification of b-PEI through functionalization with synthetic and natural polymers [ 21 ], for example, polysaccharides [ 22 , 23 , 24 ], poly (ethylene glycol) (PEG), poly ( ε -caprolactone), and poly (l-lactide) (PLLA). In the case of polysaccharides, they have attracted the interest of many researchers in gene therapy due to their advantages, such as biocompatibility, biodegradability, low toxicity, and various modification strategies.…”

Section: Introductionmentioning

confidence: 99%

“…In the case of polysaccharides, they have attracted the interest of many researchers in gene therapy due to their advantages, such as biocompatibility, biodegradability, low toxicity, and various modification strategies. Among the polysaccharides that have been used to carry out this modification are chitosan, cellulose, and natural gums [ 22 , 24 , 25 ]. Several strategies have been adopted to reduce polyethyleneimine (PEI) toxicity as the nucleic acid carrier [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…In the study by Jana et al, the authors reported that the copolymer was more biocompatible for the cells and the blood than b-PEI. Additionally, they reported that the copolymer improved the in vitro gene transfection efficiency concerning b-PEI [ 22 ]. On the other hand, Bansal et al developed a novel series of gene carriers by conjugating depolymerized guar gum with low molecular weight branched PEI to deliver nucleic acids simultaneously to macrophages and hepatocytes.…”

Section: Introductionmentioning

confidence: 99%

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Modification of Branched Polyethyleneimine Using Mesquite Gum for Its Improved Hemocompatibility

et al. 2021

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In the present study, the modification of branched polyethyleneimine (b-PEI) was carried out using mesquite gum (MG) to improve its hemocompatibility to be used in biomedical applications. In the copolymer synthesis process (carboxymethylated mesquite gum grafted polyethyleneimine copolymer (CBX-MG-PEI), an MG carboxymethylation reaction was initially carried out (carboxymethylated mesquite gum (CBX-MG). Subsequently, the functionalization between CBX-MG and b-PEI was carried out using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as crosslinking agents. The synthesis products were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and thermogravimetric analysis (TGA). Thermogravimetric analysis showed that CBX-MG and CBX-MG-PEI presented a lower decomposition temperature than MG. The CBX-MG-PEI has a high buffer capacity in the pH range of 4 to 7, similar to the b-PEI. In addition, the CBX-MG-PEI showed an improvement in hemocompatibility in comparison with the b-PEI. The results showed a non-hemolytic property at doses lower than 0.1 µg/mL (CBX-MG-PEI). These results allow us to propose that this copolymer be used in transfection, polymeric nanoparticles, and biomaterials due to its physicochemical and hemocompatibility properties.

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“…Many researchers attempted modification of guar gum [33] like carboxymethylation [5,34,35], methylation [36], cationic guar gum [4] etc. In cationic guar gum (CGG) the hydroxyl groups are replaced with trimethyl ammonium groups.…”

Section: Introductionmentioning

confidence: 99%

Approach of Electrostatic Interaction Based Layer-by-Layer Assembly of Cationic Guar Gum and Poly (Carboxylic acid)

et al. 2020

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Synthesis of a carboxymethylated guar gum grafted polyethyleneimine copolymer as an efficient gene delivery vehicle

Abstract: In the present study, a carboxymethylated guar gum-grafted-polyethyleneimine copolymer (CMGG-g-PEI) was synthesized and characterized by FT-IR, 1H NMR, XRD and zeta potential analyses.

Cited by 22 publications

References 40 publications

Plant-Based Gums and Mucilages Applications in Pharmacology and Nanomedicine: A Review

Plant-Based Gums and Mucilages Applications in Pharmacology and Nanomedicine: A Review

Modification of Branched Polyethyleneimine Using Mesquite Gum for Its Improved Hemocompatibility

Approach of Electrostatic Interaction Based Layer-by-Layer Assembly of Cationic Guar Gum and Poly (Carboxylic acid)

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