Synthesis of chitosan-coated near-infrared layered double hydroxide nanoparticles for in vivo optical imaging

Abstract

We incorporated a near-infrared (NIR) fluorescent dye, indocyanine green (ICG), in amine-modified layered double hydroxide nanoparticles (LDHs) by electrostatic attractions to render LDHs-ICG as an efficient NIR contrast agent for in vivo optical imaging. The further coating of chitosan on the external surfaces of LDHs-ICG was achieved through the cross-linking of amine-modified LDHs and different amounts of chitosan by using glutaraldehyde (GA) as a cross-linked agent. The hybridization of this organic-inorganic nanocomposite produced an efficient NIR optical contrast agent because the adsorbed ICG molecules were stabilized in the layered structures of LDHs to prevent them from leaching and/or metabolizing in physiological conditions. The cell viability and hemolysis assay also showed low cytotoxicity and low release of hemoglobin from the cell lysis of red blood cells (RBCs). The in vivo biodistribution results demonstrated that the coating of LDHs with different amounts of chitosan can develop organ-specific drug delivery systems, which can efficiently regulate the nanoparticle accumulation in various organs with un-coated LDHs-ICG targeting the liver and spleen, mono-chitosan-coated LDHs-ICG targeting the lungs, double chitosan-coated LDHs-ICG targeting the lungs and liver, and trimethylammonium (TA) modified double chitosan-coated LDHs-ICG samples targeting the liver. In addition to the high potential for the employment of chitosan-coated LDHs-ICG samples for developing contrast agents for in vivo imaging, the LDH nanoparticles can deliver therapeutic drugs to desired target organs by controlling the coating amounts of chitosan. Therefore, this approach can improve efficiency for traditional cancer diagnosis and cancer chemotherapy.

Delivery of acetylthevetin B, an antitumor cardiac glycoside, using polymeric micelles for enhanced therapeutic efficacy against lung cancer cells

“…Pluronic ® P123 (PEO 20 -PPO 70 -PEO 20 , MW 5800 Da, PEO 30 wt%) was obtained from Sigma Aldrich (St Louis, MO, USA). Chitosan (CS, MW 10 kDa, deacetylation degree: >90%) was obtained from the Zhejiang Golden Shell Pharmaceutical Co, Ltd (Yuhuan, China).…”

Bio-Layered Double Hydroxides Nanohybrids for Theranostics Applications

“…The ideal LDH nanoparticles and next generation of their bionanohybrids would be challenged in order to compromise several critical issues shared among different nanoparticles and biosystems for efficient clinical use following the approval by FDA. Organ-specific drug delivery system with noninvasive optical imaging [70] MgAl As-myc (antisense) Intercalation Gene-based cancer therapy The first work on gene delivery system with high transfection efficiency [11] (continued) MgAl 5-FU and siRNA…”

“…Wei et al recently developed innovative chitosan-coated LDH nanoparticles intercalated with a NIR fluorescent dye indocyanine green (ICG), contrast agent with US Food and Drug Administration (FDA) approval for clinical use by electrostatic interaction for in vivo NIR optical imaging [70]. The coating of chitosan (CS) was achieved on the external surface of LDHs-NH 2 -ICG by using glutaraldehyde as a cross-linked agent (Fig.…”

Functionalization of Surfaces in Layered Double Hydroxides and Hydroxide Salt Nanoparticles

“…Advanced applications have been found in the biomedical field as nanovehicles for delivery of ibuprofen, enalaprilate [17], 5-fluoracil, methotrexate [18], DNA segments [19], cosmeceuticals [20], as well as nanoparticles for diagnoses through fluorecent or magnetic analyses [21][22][23].…”