Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line

Jacob, S. Justin Packia; Finub, J.S.; Narayanan, A.

doi:10.1016/j.colsurfb.2011.11.001

Cited by 274 publications

(27 citation statements)

References 19 publications

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“…The cytotoxicity displayed by PJSNPs at lower concentrations might be linked to the phytoconstituents present in the plant extracts which are involved in AgNP formation. 62 Moreover, the cytotoxic effects of biosynthesized AgNPs against breast cancer MCF-7 cell line, 63 Hep-2 cancer cell line 64 and HeLa cell lines 65 also support our outcomes.…”

Section: Mtt Assaysupporting

confidence: 75%

Characterization and Anti-Cancerous Effect of Putranjiva roxburghii Seed Extract Mediated Silver Nanoparticles on Human Colon (HCT-116), Pancreatic (PANC-1) and Breast (MDA-MB 231) Cancer Cell Lines: A Comparative Study

Balkrishna¹,

Sharma²,

Das³

et al. 2020

IJN

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Introduction: A comparative study of Putranjiva roxburghii Wall. seed extract and developed silver nanoparticles (PJSNPs) for improving bioavailability that enhance their anti-cancer activity against HCT-116 (colon carcinoma), PANC-1 (pancreatic carcinoma), MDA-MB 231 (breast carcinoma) cell lines was performed. Materials and Methods: The green synthesis of PJSNPs (Putranjiva silver nanoparticles) was performed using PJ (Putranjiva) extract, and characterization of synthesized nanoparticles was accomplished through UV-Vis spectrum, X-ray diffraction (XRD), transmission electron microscopy, energy-dispersive X-ray spectroscopy (TEM-EDAX), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and Raman spectroscopy. Results: The results revealed that PJSNPs are homogeneous, spherical in shape,~8±2 nm in size, and negatively charged with a zeta potential of about −26.71 mV. The cytotoxicity pattern observed was AgNO 3 > PJSNPs > PJ extract. The morphological changes of the cells were observed by flow cytometry and also by the DNA ladder pattern on gel electrophoresis, which indicated that the process of cell death occurred via the apoptosis mechanism and PJSNPs were exerting late-stage apoptosis in all the tested cell lines. The small size and negative value of zeta potential could be the factors responsible for greater bioavailability and thus increased uptake by the tumor cells. Conclusion: The MTT assay and morphological changes observed by various methods indicate that the novel PJSNPs are a better anticancer agent than PJ extract. All the above properties make biologically synthesized PJSNPs an important target in the field of anticancer drug discovery.

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Section: Mtt Assaysupporting

confidence: 75%

Characterization and Anti-Cancerous Effect of Putranjiva roxburghii Seed Extract Mediated Silver Nanoparticles on Human Colon (HCT-116), Pancreatic (PANC-1) and Breast (MDA-MB 231) Cancer Cell Lines: A Comparative Study

Balkrishna¹,

Sharma²,

Das³

et al. 2020

IJN

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“…Anticancer effects of SNPs, especially against the breast cancer cell line of MCF-7 and human laryngeal carcinoma cell line of human epithelial type 2 (HEp-2) are well documented 55 . The proposed mechanism for this effect is interacting of cells and intracellular macromolecules like proteins and DNA can lead to the generation of ROS, which can provoke oxidative stress and apoptosis 56 .…”

Section: Discussionmentioning

confidence: 99%

Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line

Azizi

Ghourchian

Yazdian

et al. 2017

Sci Rep

150

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With the aim of making specific targeting of silver nanoparticles as a drug for tumor cells and developing new anticancer agents, a novel nano-composite was developed. Albumin coated silver nanoparticles (ASNPs) were synthesized, and their anti-cancerous effects were evaluated against MDA-MB 231, a human breast cancer cell line. The synthesized ASNPs were characterized by spectroscopic methods. The morphological changes of the cells were observed by inverted, florescent microscopy and also by DNA ladder pattern on gel electrophoresis; the results revealed that the cell death process occurred through the apoptosis mechanism. It was found that ASNPs with a size of 90 nm and negatively charged with a zeta-potential of about −20 mV could be specifically taken up by tumor cells. The LD50 of ASNPs against MDA-MB 231 (5 μM), was found to be 30 times higher than that for white normal blood cells (152 μM). The characteristics of the synthesized ASNPs included; intact structure of coated albumin, higher cytotoxicity against cancer cells than over normal cells, and cell death based on apoptosis and reduction of gland tumor sizes in mice. This work indicates that ASNPs could be a good candidate for chemotherapeutic drug.

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“…Piper longum leaf extract has been used for the preparation of silver nanoparticles at room temperature with an average size of 17 to 41 nm. However, it was necessary for the reduction to proceed overnight (Jacob, Finub, and Narayanan 2012). The stem latex of Euphorbia nivulia was used to synthesize spherical 18 nm silver nanoparticles.…”

Section: Syntheses At Ambient Temperaturementioning

confidence: 99%

Recent Developments and Applications in Plant-extract Mediated Synthesis of Silver Nanoparticles

Upadhyay

Verma

2015

Analytical Letters

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Green synthesis is a widely used for the preparation of silver nanoparticles. The use of plant extracts for these syntheses has become common in recent years because they are nontoxic, inexpensive, and widely available. Phytochemicals such as terpenoids, flavones, ketones, aldehydes, amides, and carboxylic acids are the primary active ingredients for nanoparticle synthesis. Reaction temperature plays an important role during the reduction process as it influences the reaction time, particle size, and the intensity of the surface plasmon resonance peak. This review presents an overview of recently developed and reported silver nanoparticle syntheses using plant extracts. The role of temperature in nanoparticles synthesis and potential Downloaded by [UQ Library] at 23:17 19 June 2015 2 applications have been reviewed. A brief description of the reaction mechanism and nanoparticle characterization techniques are also included.

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Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line

Cited by 274 publications

References 19 publications

<p>Characterization and Anti-Cancerous Effect of <em>Putranjiva roxburghii</em> Seed Extract Mediated Silver Nanoparticles on Human Colon (HCT-116), Pancreatic (PANC-1) and Breast (MDA-MB 231) Cancer Cell Lines: A Comparative Study</p>

<p>Characterization and Anti-Cancerous Effect of <em>Putranjiva roxburghii</em> Seed Extract Mediated Silver Nanoparticles on Human Colon (HCT-116), Pancreatic (PANC-1) and Breast (MDA-MB 231) Cancer Cell Lines: A Comparative Study</p>

Anti-cancerous effect of albumin coated silver nanoparticles on MDA-MB 231 human breast cancer cell line

Recent Developments and Applications in Plant-extract Mediated Synthesis of Silver Nanoparticles

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