Synthesis of Silver Nanoparticles Using Extract of Weeds and Optimized by Response Surface Methodology to the Control of Soil Pathogenic Bacteria Ralstonia solanacearum

Abstract

Extracts of leaf from five native Chilean weeds were evaluated in the biosynthesis of silver nanoparticles. The synthesis was monitored by UV-Vis spectra at 420 nm by typical formation of surface plasmon resonance. Moreover, the extract with major potential for the formation of nanoparticles was used to optimize the synthesis processes by response surface methodology, to obtain nanoparticles of small sizes by combination of three parameters (AgNO 3 and leaf extract concentrations and pH). Characterization of AgNPs was made by TEM, XRD, and DLS. The inhibitory activity was evaluated by disk diffusion method and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. Only two out of five extracts evaluated (Hypericum perforatum and Malva nicaensis) showed capacity to biosynthesize AgNPs. pH 10, AgNO 3 (1 mM), and leaf extract at 5% vv −1 were the optimal conditions to synthesize small AgNPs (< 40 nm). Inhibition zone from diffusion disk assay, MIC (30 μg mL −1) and MBC (40 μg mL −1), revealed a high antibacterial activity of AgNPs against Ralstonia solanacearum. Based on the results, the AgNPs biosynthesized from the leaf extract of H. perforatum is a promising antibacterial agent for its use in the control of R. solanacearum.

Can Diffusive Gradients in Thin Films (DGT) Technique and Chemical Extraction Methods Successfully Predict both Zn Bioaccumulation Patterns in Plant and Leaching to Groundwater in Soils Amended with Engineered ZnO Nanoparticles?

“…Over the last decade, both positive and negative effects of metal-based engineered NPs on the growth, yield, and physiology of plants have been widely reported in the literature. Plant responses to metallic and metal oxide NPs vary greatly with plant species (and even ecotypes), growth stages, soil properties, application methods, doses, and durations of NP exposure, as well as the specific surface area and size-related properties of NPs (Ebbs et al 2016;Kopittke et al 2019;Poynton et al 2019;Su et al 2019;Tortella et al 2019;Meesters et al 2019). The interactions of NPs with different soil components have a profound impact on their fate, transport, and behavior (Mitrano et al 2017;El Hadri et al 2018;Baddar et al 2019;Zhao et al 2019).…”

Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

“…Statistical optimization approaches like response surface methodology (RSM) are commonly used to investigate responses after simultaneous variation of multiple process parameters (Tortella et al 2019 ; Zaki et al 2019 ; Saeed et al 2020 ; Zaki and El-Sayed 2021 ). By employing the response surface methodology, the nanocomposite fabrication showed a better response to variations in the different investigated parameters as pH, reaction time, AgNO 3 concentration and RH concentration.…”

“…Previous reports synthesized Ag NPs with anti-bacterial activity towards R. solanacearum to control bacterial wilt (Chen et al 2016 ; Tortella et al 2019 ; Cheng et al 2020 ). The SiO 2 /Ag nanocomposite or Ag@SiO 2 core shell synthesized in previous studies displayed a wide range of antimicrobial activity towards different human and phyto-pathogen.…”

Mitigating Global Challenges: Harnessing Green Synthesized Nanomaterials for Sustainable Crop Production Systems

“…Prevents fungal spreading without affecting the leaf morphology of the plant. [84] due to its potential for simplicity, effectiveness, cleanliness, non-toxicity, and environmental acceptability in the synthesis of metallic nanoparticles (MtNPs). MtNPs can be efficiently biosynthesized using various microbes and plant extracts.…”